Antihypertensive effect of Silymarin on DOCA salt induced hypertension in unilateral nephrectomized rats

Abstract

The objective of the present investigation was to evaluate the therapeutic efficacy of Silymarin in DOCA salt induced hypertension in unilateral nephrectomized rats. Unilateral nephrectomy was performed in female Wistar rats (150-200 g). A week after unilateral nephrectomy, hypertension was induced by DOCA (25 mg/kg, once a week; s.c; for 4 weeks) dispersed in cottonseed oil. The effect of Silymarin (300 mg/kg and 500 mg/kg, p. o., for 4 weeks) was evaluated in DOCA salt induced hypertensive rats. Systolic blood pressure (BP) was measured once every week during the treatment schedule. After completion of treatment schedule, heart rate, arterial blood pressure and vascular reactivity to various drugs were recorded. Rats from individual group were sacrificed, the heart was dissected out and antioxidant enzyme level SOD, CAT, GSH and TBARS were measured. Urine excretions were measured by flame photometer. Silymarin (300, 500 mg/kg/day, p.o.) significantly (p<0.05) reduced systolic blood pressure, heart rate, basal arterial blood pressure and pressor responses to NA, Adr, PE and 5-HT in animals treated with DOCA salt as compared with DOCA-salt hypertensive rats. Silymarin significantly increased antioxidant enzyme level of SOD, CAT, GSH, urinary Na + excretion and decreased TBARS level, urinary K + excretion compared with DOCA hypertensive group. Silymarin exhibits significant antihypertensive activity in DOCA salt model of hypertension.

Simultaneous renal hypertension and type 2 diabetes exacerbate vascular endothelial dysfunction in rats

“…The study showed that in rats with simultaneous hypertension and diabetes, hypertension prevented the diabetes-induced decrease in HR. This finding is similar to that of a previous report on simultaneous DOCA-salt-induced hypertension and type 1 diabetes (Jadhav & Upasani 2011) and might be due to sympathetic hyperactivity (Souza et al 2008).…”

“…1995; Ohtomo et al. 2008) and rats with deoxycorticosteone acetate (DOCA)‐salt‐induced hypertension (Jadhav & Upasani 2011).…”

“…To the best of our knowledge, the present report represents the first study of an experimental model of simultaneous type 2 diabetes and renal hypertension. Previous studies on simultaneous models of hypertension and diabetes focused mainly on simultaneous type 1 diabetes and hypertension in spontaneous hypertensive rats (Hendriks et al 1993;Beenen et al 1996;Muharis et al 2010) and rats with renal hypertension (Mall et al 1987;Fischer et al 1992), and type 2 diabetes in spontanous hypertensive rats (Sato et al 1995;Ohtomo et al 2008) and rats with deoxycorticosteone acetate (DOCA)-saltinduced hypertension (Jadhav & Upasani 2011).…”

A Short Review on Ethnomedicinal Uses and Phytochemistry of Silybum marianum

“…Milk thistle used for alcoholic liver damage, alcoholic induced liver diseases [32]. Used against diabetes mellitus disease [33].…”

The Milk Thistle (Silybum marianum) Compound Silibinin Inhibits Cardiomyogenesis of Embryonic Stem Cells by Interfering with Angiotensin II Signaling

“…Moreover, silibinin reduced blood pressure and the incidence of postocclusion arrhythmias in spontaneously hypertensive rats, and it was suggested that this compound may be beneficial when used in hypertensive patients who develop acute myocardial infarction [6]. Silymarin exhibited significant antihypertensive activity in a DOCA salt model of hypertension [7]. In anesthetized open chest cats, silibinin lowered the amplitude and duration of diastolic blood pressure and produced a marked depression of cardiac contractility [8], suggesting that silibinin affects the hemodynamic properties of the heart.…”