Antihypoxic Action of Panax Japonicus, Tribulus Terrestris and Dioscorea Deltoidea Cell Cultures: In Silico and Animal Studies

Abstract

Chemical diversity of secondary metabolites provides a considerable variety of pharmacological actions with a significant extension due to their combinations in plant extracts. Production of plant‐derived medicinal products in cell cultures has advantages because of the efficient use of different biotic and abiotic elicitors and better control of the developmental processes. Using PASS software, we predicted biological activity spectra for phytoconstituents identified in cell cultures of Panax japonicus (12 molecules), Tribulus terrestris (4 molecules), and Dioscorea deltoidea (3 molecules). Mechanisms of action associated with the antihypoxic effect were predicted for the majority of molecules. PharmaExpert software allowed analyzing possible synergistic or additive effects of the combinations of phytoconstituents associated with the antihypoxic action. Experimental studies of the antihypoxic effect of the plants′ extracts in water and ethanol have been performed in 3 animal models: Acute asphyctic hypoxia (AAH), Acute haemic hypoxia (AHeH), and Acute histotoxic hypoxia (AHtH). Effects of Panax japonicus and Tribulus terrestris preparations exceeded the activity of the reference drug Mexidol in the AHtH model. In the AHeH model, all preparations demonstrated moderate activity; the most potent has been observed for Dioscorea deltoidea. Thus, we found that experimental studies in animal models have confirmed the in silico prediction.

Effect of Phytopreparations Based on Bioreactor-Grown Cell Biomass of Dioscorea deltoidea, Tribulus terrestris and Panax japonicus on Carbohydrate and Lipid Metabolism in Type 2 Diabetes Mellitus

“…The fifth (n = 10) and sixth (n = 10) groups received an equivalent volume of 0.9% sodium chloride. The doses of cell samples were selected based on the results of the previous study [32].The blood-glucose level was measured before the start of the experiment and after oral administration of cell preparation or metformin every 30 min for 2.5 h.…”

“…Suspension cell cultures with the ability to produce triterpene and steroidal glycosides have been developed in our laboratory as a sustainable source of biomass and bioactive ingredients of these species [26][27][28][29][30], and their biochemical profiles have been investigated by using various methods [21,[28][29][30][31]. Recently, Lagunin et al [32] suggested high antihypoxic activity of phytopreparations based on P. japonicus, D. deltoidea and T. terrestris cell cultures that, in the case of P. japonicus and T. terrestris, exceeded the effect of the reference drug Mexidol. In addition, the cell biomass of D. deltoidea showed low toxicity in rats when administered orally even at high doses, up to 5 g/kg animal body weight [25].…”

Antitumor Profile of Carbon-Bridged Steroids (CBS) and Triterpenoids

“…Their pharmacological profile is presented in Tables 16 and 17. Carbon-bridged steroids, called taccalonolides (265)(266)(267)(268)(269)(270)(271), are a class of microtubulestabilizing agents that exhibit selective cancer-fighting properties [233]. Tacca species are known to contain highly oxygenated ixocarpalactone-type steroids, with an additional ring formed by a carbon-carbon bond between C-16 and C-24, taccalonolide A being the first example of these compounds [120].…”

“…Freely available via the Internet, PASS Online web-service [264] is used by more than thirty thousand researchers from almost a hundred countries to determine the most promising biological activities for both natural and synthetic compounds [258][259][260]265]. To reveal the hidden pharmacological potential of the natural substances, we are successfully using PASS for the past fifteen years [266][267][268][269][270].…”

A comparative assessment ofin vitrocytotoxic activity and phytochemical profiling ofAndrographis nallamalayanaJ.L.Ellis andAndrographis paniculata(Burm. f.) Nees using UPLC-QTOF-MS/MS approach

“…A higher value of Pa reects the signicant biological effect experimentally and vice versa. 48,49 The structure of all the iden-tied phytoconstituents (20 compounds) were obtained from the PubChem database. The structures were submitted using the SMILES format and were subjected to the evaluation of the biological activity spectrum, including the anticancer activity.…”