Antiinflammatory Activity of Solvent-partitioned Fractions from Atriplex gmelinii C. A. Mey. in LPS-stimulated RAW264.7 Macrophages

정희정,; Kim, Ho-Jun; Eunshin, Ju; Lee, Seulgi; Kong, Chang‐Suk; Seo, Youngwan

doi:10.5352/jls.2017.27.2.187

Journal of Life Science

2017

DOI: 10.5352/jls.2017.27.2.187

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Antiinflammatory Activity of Solvent-partitioned Fractions from Atriplex gmelinii C. A. Mey. in LPS-stimulated RAW264.7 Macrophages

정희정 정희정¹,

Ho-Jun Kim²,

Ju Eunshin³

et al.

Abstract: As a part of ongoing research to elucidate and characterize antiinflammatory nutraceuticals, the crude extracts from Atriplex gmelinii C. A. Mey. and their solvent-partitioned fractions were tested for their antiinflammatory potential in lipopolysaccharide (LPS)-stimulated RAW 264.7 mouse macrophages. The crude extracts of A. gmelinii C. A. Mey. were fractioned according to polarity with n-hexane, 85% aqueous methanol (85% aq. MeOH), n-butanol, and H2O. Their antiinflammatory activities were investigated in LP… Show more

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Cited by 5 publications

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“…2019 ), and anti-inflammation properties (Jeong et al. 2017 ), as well as its enhancement of osteoblast differentiation (Karadeniz et al. 2020 ).…”

mentioning

confidence: 99%

The complete chloroplast genome of Atriplex gmelinii C. A. Mey. ex Bong. (Amaranthaceae)

Park¹,

Kim

Min³

2022

Mitochondrial DNA Part B

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Atriplex gmelinii C. A. Mey. Ex Bong._1838 is an annual halophytic herb found in East Asia and North America. The chloroplast genome of A. gmelinii was successfully sequenced. The assembled genome (151,852 bp; GC ratio, 37.3%) is composed of four subregions, with the large single copy (LSC; 83,872 bp; 35.4%) and small single copy (SSC; 17,812 bp; 30.9%) regions separated by two regions of inverted repeat regions (25,084 bp; 42.8%). A total of 130 genes were predicted with 85 protein-coding genes, 8 rRNAs, and 37 tRNAs. The phylogenetic analyses inferred from whole chloroplast genomes of 35 species, including 34 species in Amaranthaceae and one outgroup species, suggest a close relationship between A. gmelinii and A. centralasiatica .

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“…2019 ), and anti-inflammation properties (Jeong et al. 2017 ), as well as its enhancement of osteoblast differentiation (Karadeniz et al. 2020 ).…”

mentioning

confidence: 99%

The complete chloroplast genome of Atriplex gmelinii C. A. Mey. ex Bong. (Amaranthaceae)

Park¹,

Kim

Min³

2022

Mitochondrial DNA Part B

View full text Add to dashboard Cite

show abstract

3,5-dicaffeoyl‑epi-quinic acid from Atriplex gmelinii enhances the osteoblast differentiation of bone marrow-derived human mesenchymal stromal cells via WnT/BMP signaling and suppresses adipocyte differentiation via AMPK activation

Karadeniz

Lee

et al. 2020

Phytomedicine

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Phytochemical investigation and anti-inflammatory potential of Atriplex leucoclada Boiss

Ahmed,

Mohamed,

Amin

et al. 2023

BMC Complement Med Ther

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Background The plant kingdom has long been considered a valuable source for therapeutic agents, however, some plant species still untapped and need to be phytochemically and biologically explored. Although several Atriplex species have been investigated in depth, A. leucoclada, a halophytic plant native to Saudi Arabian desert, remains to be explored for its phytochemical content and biological potentials. Herein, the current study investigated the metabolic content and the anti-inflammatory potential of A. leucoclada. Methods Powdered aerial parts of the plant were defatted with n-hexane then the defatted powder was extracted with 80% methanol. n-Hexane extract (ATH) was analyzed using GC–MS, while the defatted extract (ATD) was subjected to different chromatographic methods to isolate the major phytoconstituents. The structures of the purified compounds were elucidated using different spectroscopic methods including advanced NMR techniques. Anti-inflammatory activity of both extracts against COX-1 and COX-2 enzymes were examined in vitro. Molecular docking of the identified compounds into the active sites of COX-1 and COX-2 enzymes was conducted using pdb entries 6Y3C and 5IKV, respectively. Results Phytochemical investigation of ATD extract led to purification and identification of nine compounds. Interestingly, all the compounds, except for 20-hydroxy ecdysone (1), are reported for the first time from A. leucoclada, also luteolin (6) and pallidol (8) are isolated for the first time from genus Atriplex. Inhibitory activity of ATD and ATH extracts against COX-1 and COX-2 enzymes revealed concentration dependent activity of both fractions with IC50 41.22, 14.40 μg/ml for ATD and 16.74 and 5.96 μg/ml for ATH against COX-1 and COX-2, respectively. Both extracts displayed selectivity indices of 2.86 and 2.80, respectively as compared to 2.56 for Ibuprofen indicating a promising selectivity towards COX-2. Molecular docking study supported in vitro testing results, where purified metabolites showed binding affinity scores ranged from -9 to -6.4 and -8.5 to -6.6 kcal/mol for COX-1 and 2, respectively, in addition the binding energies of GC–MS detected compounds ranged from -8.9 to -5.5 and -8.3 to -5.1 kcal/mol for COX-1 and 2, respectively as compared to Ibuprofen (-6.9 and -7.5 kcal/mol, respectively), indicating high binding affinities of most of the compounds. Analysis of the binding orientations revealed variable binding patterns depending on the nature of the compounds. Our study suggested A. leucoclada as a generous source for anti-inflammatory agents.

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Antiinflammatory Activity of Solvent-partitioned Fractions from Atriplex gmelinii C. A. Mey. in LPS-stimulated RAW264.7 Macrophages

Cited by 5 publications

References 17 publications

The complete chloroplast genome of Atriplex gmelinii C. A. Mey. ex Bong. (Amaranthaceae)

The complete chloroplast genome of Atriplex gmelinii C. A. Mey. ex Bong. (Amaranthaceae)

3,5-dicaffeoyl‑epi-quinic acid from Atriplex gmelinii enhances the osteoblast differentiation of bone marrow-derived human mesenchymal stromal cells via WnT/BMP signaling and suppresses adipocyte differentiation via AMPK activation

Phytochemical investigation and anti-inflammatory potential of Atriplex leucoclada Boiss

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