Antiinflammatory Compounds fromPlagiorhegma dubiumCell Culture¹

Abstract

Successive fractionation of a crude methanolic extract of cultured PLAGIORHEGMA DUBIUM cells, followed by assay of the biological activity using cobra venom factor induced paw oedema, led to the detection and isolation of three compounds with antiinflammatory activity. The substances were identified as the protoberberine alkaloid jatrorrhizine and the lignaneglucosides dehydrodiconiferyl-alcohol-4-beta- D-glucoside and its isomer dehydrodiconiferyl-alcohol-gamma-beta- D-glucoside, the latter a compound not previously described in the literature.

Antiprotozoal activity of Melampyrum arvense and its metabolites

“…Podophyllotoxin was the standard drug used. (Chaudhuri et al, 1981), mussaenoside (3) (El-Naggar and Beal, 1980), mussaenosidic acid (4), 8-epi-loganin (5) (Boros and Stermitz 1990), flavonoids apigenin (6), luteolin (7), luteolin 7-O-β-glucopyranoside (8) (Markham and Chari, 1982), a lignan glycoside, dehydrodiconiferyl alcohol 9-O-β-glucopyranoside (9) (Arens et al, 1985), an aromatic acid, benzoic acid (10) by comparison of their spectroscopic data (1D and 2D NMR, ESIMS and [α] D ) with those published in the literature. As displayed in Table 1, the CHCl 3 subextract was more active against all parasitic protozoa than the H 2 O extract.…”

Novel Antiinflammatory Flavonoids fromPodophyllum versipelleCell Culture11

“…In 1984, cell suspensions from our callus cell line 729-01 were established under conditions published previously (3). The suspension culture consists of small clusters and aggregates of friable texture.…”

“…Plant cell cultures are an interesting source of pharmacologically active compounds because they yield substances which cannot be detected in the original plant or may even be completely new (1,2,3). Therefore we decided to screen callus cultures from different plant species for biological activity.…”

Formation of Secondary Metabolites in Cultured Plant Cells and Its Impact on Pharmacy

“…The product levels needed for process development would only depend on the pharmacological qualities of the novel compound as compared to known drugs applied for the same purpose. With the financial support of the German government the Nattermann Company in Cologne has screened several hundred cell cultures for novel pharmacologically active compounds using sensitive Arens et al (1985) 1. Berlin assays such as an opiate receptor binding assay (Arens et al 1982).…”