Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice

Penido, Carmen; Conte, Fernando de Paiva; Chagas, Maria S.S.; Rodrigues, Carina; Pereira, João Baptista Borges; Henriques, Maria das Graças

doi:10.1007/s00011-006-5161-8

Cited by 112 publications

(83 citation statements)

References 33 publications

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“…Especially the oil extract from seeds of C. guianensis has been empirically used in the treatment of arthritis, rheumatism, infections, wounds, bruises, skin diseases and as an insect repellent (Brito et al 2013, Hammer andJohns 1993). Scientific studies also identify antiplasmodial (Bickii et al 2000, Miranda et al 2012), analgesic (Penido et al 2005, anti-inflamatory (Penido et al 2006), anti-allergic (Ferraris et al 2011 activities from the C. guianensis oil. Besides the widespread use of C. guianensis by the population, there is little information about the mutational potential.…”

Section: Introductionmentioning

confidence: 99%

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Lemes

Chaves

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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The aim of this study was to evaluate the possible protective of C. guianensis oil against MMC and CP, which are direct-and indirect-acting chemical mutagens, using the micronucleus test. Three experiments were performed. First the C. guianensis oil was co-administered to mice at doses of 250, 500 and 1000 mg/ kg bw with 4 mg/kg bw MMC or 50 mg/kg bw CP. Second, the mutagenic drug (CP) was administered ip 50 mg/kg bw and after 6 and 12 hours 250 and 500 mg/kg bw of C. guianensis oil were administered. In the last, C. guianensis oil was administrated (250 and 500 mg/kg bw) during five days and after it was administered ip 50 mg/kg bw CP. The results obtained showed that the C. guianensis oil is not cytotoxic neither genotoxic to mouse bone marrow. Regarding the antimutagenic effect, all doses of C. guianensis oil were significantly (p < 0.05) effective in reducing the frequency of micronucleated polychromatic erythrocytes, when compared with MMC or CP alone. Based on these results, our results suggest that the C. guianensis oil shows medicinal potential as an antimutagenic agent, modulating the mutagenicity caused by both direct-and indirect-acting chemical mutagens, in a mammalian model.

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Section: Introductionmentioning

confidence: 99%

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Lemes

Chaves

Silva

et al. 2017

An. Acad. Bras. Ciênc.

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“…Other studies proved that this oil has analgesic, anti-allergic, and antihistaminic activity, inhibiting the formation of local edema (PENNAFORTE, 2003). Antiedema and analgesic properties of andiroba oil and a derived fraction of this oil, rich in tetranortriterpenoids, were confirmed in Swiss C57/BL10 strain mice with induced pleurisy and edema of the limbs (PENIDO et al, 2005) and zymozan-induced arthritis (PENIDO et al, 2006).…”

Section: Introductionmentioning

confidence: 83%

Effects of topical application of pure and ozonized andiroba oil on experimentally induced wounds in horses

Araújo¹,

Teixeira²,

Lacerda³

et al. 2017

Braz. J. Vet. Res. Anim. Sci.

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The aim of this study was to evaluate clinical and histopathological aspects of topical application of pure and ozonized andiroba oil (Carapa guianensis Aublet.) on the healing process of wounds in healthy horses. Eight 6.25 cm 2 wounds were surgically produced on each horse, from the cranial region to the sacrum, being four wounds on each side of the lumbar region. In three animals, left side was used for macroscopic observations and area measurement and right side was used for histopathological analysis. For the other two animals, evaluations were inverted. The beginning of the topical treatment occurred 12 hours after surgical induction of the injuries and was maintained daily until complete healing of the wounds, using saline solution (GC), ozonized saline solution (GO) sequentially and bilaterally in the craniocaudal direction, pure andiroba oil (GAP) and ozonized andiroba oil (GAO). Randomly, the sequence of the treatments was modified. Macroscopic and histopathological analyses were performed at 3, 7, 14, and 21 days after surgery. The time for complete healing of all wounds was recorded. A wound contraction of 67.75% for GC, 65.26% for GO, 67.91% for GAP, and 69.84% for GAO were recorded. Histopathologic evaluation revealed that wounds from the GAO and GAP had an advanced epithelialization, fibroblast proliferation and collagen deposition, moderate vascular proliferation, and presence of PMN infiltrate and discrete viewing of MN. It was possible to conclude that all treatments had benefits when comparing to control group, concluding that both pure and ozonized andiroba oil may be good options for treating wounds in horses. Keywords: Carapa guianensis. Healing process. Equines. Wounds. Ozone. ResumoEste trabalho realizou uma avaliação clínica e histopatológica da aplicação tópica do óleo de andiroba (Carapa guianensis Aublet), puro e ozonizado, no processo de cicatrização de feridas em cinco equinos saudáveis. Oito feridas de 6,25 cm 2 foram induzidas cirurgicamente, quatro de cada lado da região lombar, craniais em relação à região sacral. Em três animais, o lado esquerdo foi destinado à avaliação macroscópica e mensuração de área, enquanto o lado direito foi destinado à análise histopatológica. Nos outros dois animais, as avaliações foram invertidas. O tratamento tópico foi iniciado 12 horas após a indução cirúrgica e foi mantido diariamente até a completa cicatrização das feridas. Foram usados, sequencialmente e bilateralmente, no sentido craniocaudal: solução salina (GC), solução salina ozonizada (GO), óleo de andiroba puro (GAP) e óleo de andiroba ozonizado (GAO). Aleatoriamente, a sequência de tratamentos foi modificada. As análises macroscópicas e microscópicas foram realizadas 3, 7, 14 e 21 dias após a cirurgia, e o tempo total para cicatrização registrado. A contração da ferida foi de 67,75% para GC, 65,26% para GO, 67,91% para GAP, e 69,84% para GAO. A avaliação histopatológica demonstrou que as feridas tratadas com GAO e GAP apresentaram uma avançada epitelização, proliferação fibroblás...

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“…Dentre os modelos animais estudados, o modelo de artrite induzida por zimosan tem sido amplamente utilizado para a avaliação do efeito anti-inflamatório de diversos produtos naturais (Penido et al, 2006;Paiva et al, 2011;Yamada et al, 2013). Neste modelo o zimosan induz um processo inflamatório agudo nas articulações e, poucas horas após a aplicação intra-articular deste estímulo, são encontrados parâmetros inflamatórios semelhantes aos da AR, com formação de edema, infiltração maciça de leucócitos (principalmente neutrófilos) nos tecidos e fluidos sinoviais e liberação de citocinas pró-inflamatórias (Penido et al, 2006;Takahashi et al, 2011 (Carlos et al, 2014).…”

Section: Discussionunclassified

“…Neste modelo o zimosan induz um processo inflamatório agudo nas articulações e, poucas horas após a aplicação intra-articular deste estímulo, são encontrados parâmetros inflamatórios semelhantes aos da AR, com formação de edema, infiltração maciça de leucócitos (principalmente neutrófilos) nos tecidos e fluidos sinoviais e liberação de citocinas pró-inflamatórias (Penido et al, 2006;Takahashi et al, 2011 (Carlos et al, 2014). Além disso, a gravidade do quadro clínico desta doença está intimamente associada ao influxo de neutrófilos para o local da inflamação (Bezerra et al, 2007).…”

Section: Discussionunclassified

“…Além disso, outros estudos já mostraram que o ácido cafeico foi capaz de reduzir as concentrações de TNF-α, IL-6 e IL-1β em modelo de inflamação cutânea em camundongos (Zhang et al, 2014), indicando a capacidade deste composto em atuar sobre a liberação destas citocinas em processos inflamatórios agudos. Tais citocinas são mediadores intimamente envolvidos na patogênese da artrite e níveis aumentados destes mediadores são encontrados nas articulações de pacientes com AR, o que contribui para o recrutamento de mais células para o local da inflamação, além de danos nas articulações (Penido et al, 2006 Landi-Librandi et al, 2012a,b;Ji et al, 2013;Rezaei-Sadabady et al, 2014 A análise qualitativa dos picos foi realizada através da avaliação dos tempos de retenção e espectros de absorção do ultravioleta das amostras em comparação aos obtidos para os padrões cromatográficos sob as mesmas condições cromatográficas.…”

Section: Discussionunclassified

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O extrato etanólico das folhas de Baccharis dracunculifolia (Asteraceae) modula funções efetoras de neutrófilos: um promissor agente fitoterápico para o tratamento de processos inflamatórios mediados por estas células

Rinhel¹

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RibeirãoNeutrophils are phagocytic immune cells mobilized primarily to fight against infections. These cells are capable to kill microorganisms through degranulation, which leads to the release of antimicrobial molecules, and production of reactive oxygen species. Although neutrophil recruitment is essential for host protection, excessive activation of these cells culminates in intense discharge of cytotoxic molecules to the extracellular milieu, which can elicit the development of and/or aggravate the clinical condition in autoimmune and inflammatory diseases such as rheumatoid arthritis. Modulation of the effector functions of neutrophils is a possible therapeutic approach to treat these diseases. Previous studies have shown that the ethanolic extract of Baccharis dracunculifolia leaves (EEBd) has a great therapeutic potential due to its significant antioxidant effect towards the oxidative metabolism of neutrophils. To continue investigating the anti-inflammatory and/or immunomodulatory action of such extract, the first part of the present work examined its modulator effect on three important effector functions of human neutrophils that mediate recognition and clearance of pathogens -phagocytosis, degranulation, and microbial killing -and on the activity of lysosomal enzymes. EEBd inhibited the oxidative metabolism of neutrophils without affecting their defense ability, depending on the extract concentration used. To propose possible chemical markers for the biological effects of the extract, the second part of this study evaluated the antioxidant activity of seven compounds isolated from EEBd -caffeic acid, coumaric acid, ferulic acid, cinnamic acid, aromadendrin-4'-methyl ether, isosakuranetin, and hispidulin -towards the oxidative metabolism of neutrophils. The results pointed to caffeic acid as a potential chemical marker for this biological activity. The third part of this investigation aimed to improve the bioavailability and favor the therapeutic use of the natural products studied. EEBd and caffeic acid were incorporated into liposomal carrier systems. The incorporation of both samples into liposomes did not alter their antioxidant activity towards neutrophils, as compared with their respective free forms. Finally, this study examined the therapeutic effect of EEBd and caffeic acid, both in the free form and incorporated into liposomes, in an animal model of arthritis. The free samples markedly improved the joint inflammatory parameters: they reduced edema and migration of total cells and neutrophils, and slightly lowered the levels of the inflammatory cytokines tumor necrosis factor  and interleukin 6 and 1 (TNF-, IL-6, and IL-1, respectively). In addition, incorporation of EEBd and caffeic acid into liposomes can help to reduce their concentrations required to achieve the desired therapeutic effect. Based on the set of results obtained in this study, we conclude that EEBd has a great potential to become a therapeutic adjuvant in the treatment of neutrophil-mediated inflammatory diseases, and that...

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Antiinflammatory effects of natural tetranortriterpenoids isolated from Carapa guianensis Aublet on zymosan-induced arthritis in mice

Abstract: Taken together, these results indicate that TNTP present an important antiinflammatory effect, inhibiting zymosan-induced arthritis in mice via the impairment of TNF-alpha, IL-1beta and CXCL8/IL-8 generation, as well as NFkappaB signaling pathway.

Cited by 112 publications

References 33 publications

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Antigenotoxicity protection of Carapa guianensis oil against mitomycin C and cyclophosphamide in mouse bone marrow

Effects of topical application of pure and ozonized andiroba oil on experimentally induced wounds in horses

O extrato etanólico das folhas de Baccharis dracunculifolia (Asteraceae) modula funções efetoras de neutrófilos: um promissor agente fitoterápico para o tratamento de processos inflamatórios mediados por estas células

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