Antiinflammatory principles of Atractylodes rhizomes.

Endo, Katsuya; Taguchi, Takashi; F, Taguchi; Hikino, Hiroshi; Yamahara, Johji; Fujimura, Hajimè

doi:10.1248/cpb.27.2954

Cited by 95 publications

(72 citation statements)

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“…The MS spectra of the dominant constituents of the PE fraction revealed six compounds. These compounds include two sesquiterpene compounds: eudesm-4(15),7-diene-9a,11-diol (Wang et al 2008a, b) and eudesm-4(15)-ene-7a,11-diol (Wang et al 2008a, b); three sesquiterpene lactone compounds: atractylenolid I (Meng et al 2010;Endo et al 1979), atractylenolid III (Wang et al 2007;Li et al 2002;Wu et al 2004), and 3-b-acetyl-atractylenolid III (Wang et al 2007); and one polyacetylenic compound: 4,6,12-tetradecatriene-8,10-diyne-1,3,14-triol (Nakai et al 2003).…”

Section: Changes In Mitochondrial Transmembrane Potentialmentioning

confidence: 99%

Selective fraction of Atractylodes lancea (Thunb.) DC. and its growth inhibitory effect on human gastric cancer cells

et al. 2013

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The purpose of this study was to investigate the pharmacological effect of fraction of Atractylodes lancea (Thunb.) DC. (A. lancea) extract. In this study, we isolated different polarity fractions, including petroleum ether (PE), ethyl acetate, nbutanol, and the remaining H 2 O fractions from the water extract of A. lancea. The antigastric cancer properties of the different fractions in BGC-823 and SGC-7901 cells were evaluated. Apoptotic cells were treated with PE fraction and stained with Hoechst 33342 and 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide. The cell cycle was analyzed via flow cytometry. The main compounds of PE fraction were determined by HPLC-ESI-MS. Results of this study showed that the PE fraction of A. lancea inhibited the growth of BGC-823 and SGC-7901 cells in a dose-and time-dependent manner. The morphological and mitochondrial transmembrane potential changes suggested that the cells showed preliminary apoptosis characteristics after treatment with the three different polarities. The main compounds of PE fraction include two sesquiterpene compounds: eudesm-4(15),7-diene-9a,11-diol and eudesm-4(15)-ene-7a,11-diol; three sesquiterpene lactone compounds: atractylenolid I, atractylenolid III and 3-b-acetyl-atractylenolid III and one polyacetylenic compound: 4,6,12-tetradecatriene-8,10-diyne-1,3,14-triol.

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Section: Changes In Mitochondrial Transmembrane Potentialmentioning

confidence: 99%

Selective fraction of Atractylodes lancea (Thunb.) DC. and its growth inhibitory effect on human gastric cancer cells

et al. 2013

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“…The molecular formula of compound 1 was determined on the basis of the spectral data ( 1 Hand 13 C-NMR, MS). 19,20) Cells and Culture Melan-a melanocytes were a kind gift from Dr. Bennette D.C. (St. George's Hospital, London, U.K. line originally derived from C57BL mice and more similar to the primary mouse melanocytes than tumorigenic B16 melanoma cell. 21) Melan-a cells were grown in a humidified incubator at 37°C in a humidified atomosphere of 5% CO 2 .…”

Section: Materials Atractylodis Rhizoma Alba Was Purchased Atmentioning

confidence: 99%

“…The structure of compound 1 was determined by comparison of its MS, UV, and NMR spectral data (Table 1) with those reported in the literature. 19,20) Compound 1 was a colorless oil. The EI-MS spectrum showed a molecular ion peak at m/z 218.34.…”

Section: Isolation and Structural Identification Of Compoundmentioning

confidence: 99%

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Inhibition of Melanogenesis by Selina-4(14),7(11)-dien-8-one Isolated from Atractylodis Rhizoma Alba

Chang¹,

Kim²,

MinHwan³

et al. 2007

Biological & Pharmaceutical Bulletin

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The level of UV radiation emitted by the sun is increasing due to the destruction of the ozone layer. Our skin is more exposed to UV radiation and often suffers from various harmful effects of UV. Melanin prodcution in human skin is an important defense mechanism against UV and a major determinant of skin color.1,2) Melanocytes residing within the basal layer of the epidermis synthesize melanin in specialized organelles, designated as melanosomes, by coordinated action of various melanogenic enzymes such as tyrosinase, tyrosinase-related protein 1 (TRP-1), and tyrosinase-related protein 2 (TRP-2).3-8) Then, melanin-containing melanosomes are transferred to keratinocytes, providing protection against UV radiation and eventually disappear with desquamation of the skin.9,10) Despite the protective role of melanins, abnormal hyperpigmentation such as freckles, chloasma, and other forms of melanin hyperpigmentation sometimes can be serious aesthetic problems. Therefore potent active agents for the improvement of hyperpigmentation have been sought for cosmetic use. Many chemicals such as hydroquinone, arbutin, kojic acid, and ascorbic acid are well known for their melanogenic-inhibitory functions. [11][12][13][14] Even though these chemicals are often used in many skin-lightening, cosmetic formulations, they have some disadvantages such as poor safety and limited effectiveness. [15][16][17] In this study, we screened more than 200 medicinal herbal extracts that have been known for their skin-lightening effects to develop a skin-lightening agent from traditional medicinal plants and found that Atractylodis Rhizoma Alba, which has been used in the treatment and prevention of diabetes and ulceration, has strong melanogenic-inhibitory activity.18) Its active compound was purified using bioactivityguided fractionation and identified as selina-4(14),7(11)-dien-8-one (compound 1) by spectroscopic methods.The effects of compound 1 were evaluated on melan-a cells. Compound 1 markedly inhibited melanogenesis of melan-a cells by regulating the expression of melanogenic enzymes. Isolation of Compound 1 (Selina-4(14),7(11)-dien-8-one) Atractylodis Rhizoma Alba (2 kg) was extracted with methanol (three times, each 5 l) at 60°C. The concentrated methanol extract (350 g) was added to water and then partitioned successively with n-hexane, ethyl acetate (EtOAc), and 1-butanol. The n-hexane layer (35 g), which showed significant melanogenic-inhibitory activity (IC 50 ϭ100 mg/ml), was fractionated using silica gel column chromatography with n-hexane-ethyl acetate stepwise elution (n-hexane : EtOAcϭ80 : 1-2 : 1). Five fractions were obtained and fraction III showed the strongest activity (IC 50 ϭ20 mg/ml). This fraction was subjected to silica gel column chromatography with n-hexane-ethyl acetate (40 : 1-1 : 1). Compound 1 was further purified by HPLC with n-hexane-ethyl acetate (90 : 1) as eluting solvents (Chart 1). The molecular formula of compound 1 was determined on the basis of the spectral data ( LG Household & Healthcare Ltd.; K...

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“…Dry rhizomes of A. macrocephala are rich in sesquiterpenes and acetylenic compounds (Endo et al, 1979;Chen 1987;Huang et al, 1992;Lin et al, 1997). Typical polysaccharides atractan A, B, and C, present in A. macrocephala, have been reported to exhibit hypoglycaemic activities (Wang et al, 2000;Jia et al, 2003).…”

Section: Introductionmentioning

confidence: 99%

Chemical and ecological evaluation of a genuine chinese medicine <i>Atractylodes macrocephala</i> Koidz

Zhou¹,

Qu²,

Yu³

2011

Afr. J. Trad. Compl. Alt. Med.

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Antiinflammatory principles of Atractylodes rhizomes.

Cited by 95 publications

References 0 publications

Selective fraction of Atractylodes lancea (Thunb.) DC. and its growth inhibitory effect on human gastric cancer cells

Selective fraction of Atractylodes lancea (Thunb.) DC. and its growth inhibitory effect on human gastric cancer cells

Inhibition of Melanogenesis by Selina-4(14),7(11)-dien-8-one Isolated from Atractylodis Rhizoma Alba

Chemical and ecological evaluation of a genuine chinese medicine <i>Atractylodes macrocephala</i> Koidz

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