Selective fraction of Atractylodes lancea (Thunb.) DC. and its growth inhibitory effect on human gastric cancer cells

Abstract

The purpose of this study was to investigate the pharmacological effect of fraction of Atractylodes lancea (Thunb.) DC. (A. lancea) extract. In this study, we isolated different polarity fractions, including petroleum ether (PE), ethyl acetate, nbutanol, and the remaining H 2 O fractions from the water extract of A. lancea. The antigastric cancer properties of the different fractions in BGC-823 and SGC-7901 cells were evaluated. Apoptotic cells were treated with PE fraction and stained with Hoechst 33342 and 5,5,6,6-tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide. The cell cycle was analyzed via flow cytometry. The main compounds of PE fraction were determined by HPLC-ESI-MS. Results of this study showed that the PE fraction of A. lancea inhibited the growth of BGC-823 and SGC-7901 cells in a dose-and time-dependent manner. The morphological and mitochondrial transmembrane potential changes suggested that the cells showed preliminary apoptosis characteristics after treatment with the three different polarities. The main compounds of PE fraction include two sesquiterpene compounds: eudesm-4(15),7-diene-9a,11-diol and eudesm-4(15)-ene-7a,11-diol; three sesquiterpene lactone compounds: atractylenolid I, atractylenolid III and 3-b-acetyl-atractylenolid III and one polyacetylenic compound: 4,6,12-tetradecatriene-8,10-diyne-1,3,14-triol.

The Suppressive Effects of the Petroleum Ether Fraction fromAtractylodes lancea(Thunb.) DC. On a Collagen-Induced Arthritis Model

“…lancea rhizome. Its major properties of interest are its antiinflammatory and anticancer activities (Kim et al ., ; Zhao et al ., ). Recently, the A .…”

“…The A. lancea rhizome (Cangzhu), which is derived from the rhizome of A. lancea (Thunb.) DC., has been used to treat digestive disorders, rheumatic diseases, night blindness, and influenza (Zhao et al, 2014). Numerous in vitro studies have described the different biological effects of the A. lancea rhizome.…”

“…Numerous in vitro studies have described the different biological effects of the A. lancea rhizome. Its major properties of interest are its antiinflammatory and anticancer activities (Kim et al, 2014;Zhao et al, 2014). Recently, the A. lancea rhizome has been suggested to have many properties that could be beneficial in the Figure 7.…”

“…The Atractylodes lancea rhizome is listed in the Chinese, Korean, and Japanese pharmacopoeias and is traditionally used for the treatment of digestive disorders, rheumatic diseases, night blindness, and influenza (Zhao et al ., ). Earlier phytochemical studies showed that A .…”

“…The Atractylodes lancea rhizome is listed in the Chinese, Korean, and Japanese pharmacopoeias and is traditionally used for the treatment of digestive disorders, rheumatic diseases, night blindness, and influenza (Zhao et al, 2014). Earlier phytochemical studies showed that A. lancea contained polyacetylenes, sesquiterpenes, sesquiterpenoids, and sesquiterpene glycosides (Wang et al, 2008;Yao and Yang, 2014).…”