Antimalarial Activity and Toxicological Assessment of <i>Betula alnoides</i> Extract against <i>Plasmodium berghei</i> Infections in Mice

Chaniad, Prapaporn; Techarang, Tachpon; Phuwajaroanpong, Arisara; Punsawad, Chuchard

doi:10.1155/2019/2324679

Cited by 25 publications

(34 citation statements)

References 17 publications

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“…These results were consistent with those of a previous study regarding the in vivo antimalarial activity of the Betula alnoides aqueous stem extract, which also exhibited significant suppressive activity against P. berghei in a dosedependent manner. 23 To confirm extract safety, an acute toxicity test was performed by orally administering the extract at a dose of 2000 mg/kg body weight. Manifestations of toxicity or mortality were not observed.…”

Section: Discussionmentioning

confidence: 99%

“…berghei in a dose-dependent manner. 23 To confirm extract safety, an acute toxicity test was performed by orally administering the extract at a dose of 2000 mg/kg body weight. Manifestations of toxicity or mortality were not observed.…”

Section: Discussionmentioning

confidence: 99%

“…22 Rodent parasites of Plasmodium berghei strain ANKA were obtained from BEI Resources, NIAID, NIH: P. berghei , strain ANKA, MRA-311, contributed by Thomas F. McCutchan. Five groups of ICR mice (5 mice each) were randomly created, including 3 groups of mice treated with 200, 400 and 600 mg/kg body weight ethanolic P. cablin extract according to previous studies 23 – 25 and positive and negative control groups. Briefly, mice were inoculated intraperitoneally with 200 µl of P. berghei ANKA-infected red cells containing 10 7 cells/ml on day 0.…”

Section: Methodsmentioning

confidence: 99%

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In VitroandIn VivoAntimalarial Activities and Toxicological Assessment ofPogostemon Cablin(Blanco) Benth

Phuwajaroanpong

Chaniad

Horata

et al. 2020

J Evid Based Complementary Altern Med

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The aim of this study was to investigate the antimalarial activities and toxicity of Pogostemon cablin extracts. In vitro activities against the chloroquine-resistant Plasmodium falciparum K1 strain were assessed by using the Plasmodium lactate dehydrogenase enzyme (pLDH) assay, while in vivo activity against the Plasmodium berghei ANKA strain in mice was investigated using a 4-day suppressive test. The in vitro and in vivo toxicity were determined in Vero cells and mice, respectively. The ethanolic extract possessed antimalarial activity with an IC50 of 24.49 ± 0.01 µg/ml, whereas the aqueous extract showed an IC50 of 549.30 ± 0.07 µg/ml. Cytotoxic analyses of the ethanolic and aqueous extracts revealed a nontoxic effect on Vero cells at a concentration of 80 µg/ml. Based on a preliminary study of in vitro antimalarial activity, the ethanolic extract was chosen as a potential agent for further in vivo antimalarial activity analysis in mice. The ethanolic extract, which showed no toxic effect on mice at a dose of 2000 mg/kg body weight, significantly suppressed parasitemia in mice by 38.41%, 45.12% and 89.00% at doses of 200, 400 and 600 mg/kg body weight, respectively. In conclusion, this study shows that the ethanolic P. cablin extract possesses in vitro and in vivo antimalarial activity without toxic effects.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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In VitroandIn VivoAntimalarial Activities and Toxicological Assessment ofPogostemon Cablin(Blanco) Benth

Phuwajaroanpong

Chaniad

Horata

et al. 2020

J Evid Based Complementary Altern Med

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“…+ Extract) were treated orally for 4 weeks with the extract of B. etnensis at the dose of 0.5 g/kg, p.o. The dose was determined according to the literature data [ 23 , 24 ]. Rats were placed in individual metabolic cages; in all four groups of animals, blood glucose and insulin, body weight, volume of excreted urine, amount of water and food consumed were assessed at 8, 15, 21 and 28 days.…”

Section: Methodsmentioning

confidence: 99%

The Antioxidant Activities of Betula etnensis Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats

et al. 2020

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Pathophysiological mechanisms correlating diabetes mellitus with associated complications are still not completely clear, even though oxidative stress seems to play a pivotal role. Literature data suggest that cell damages induced by hyperglycemia, although multifactorial, have a common pathway in oxidative/nitrosative stress. The present study evaluated the effects of Betula etnensis Raf. bark extract, a plant belonging to the Betulaceae family endemic to Sicily, on oxidative stress and in preventing and/or retarding diabetes-associated complications in streptozotocin diabetic rats treated with the extract at dose of 0.5 g/kg body weight per day for 28 consecutive days. The extract administration significant decreased food and water intake, fasting blood glucose, weight loss and polyuria, compared with untreated diabetic animals. Furthermore, oxidative stress markers particularly, lipid hydroperoxides (LOOH) and nitrite/nitrate levels, non-proteic thiol groups (RSH), γ-glutamyl-cysteine-synthetase (γ-GCS) activities and expression, heme oxygenase-1 (HO-1), endothelial and inducible nitric oxide synthases (i-NOS e-NOS) expression, significantly changed by streptozocin treatment, were markedly restored both in plasma and tissues together with nuclear sirtuins activity (Sirt1). Results suggested that B. etnensis bark alcoholic extract is able to counteract oxidative stress and to ameliorate some general parameters related to diabetes.

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“…All the mice were kept alive until the 28th day to assess the survival time (TS) [22,23]. In vivo antiplasmodial activity of extracts were classified as moderate, good, and very good if an extract displayed respective percent parasite suppression equal to or greater than 50% at doses of 500, 250, and 100 mg/kg body weight per day, respectively [24].…”

Section: Antiplasmodial Activity-4-day Suppressive Testmentioning

confidence: 99%

Antiplasmodial, antioxidant and toxicological study of leaves extracts of Dalbergia katangensis Lecheneaud (Fabaceae) from Eastern DR Congo

Valentin¹,

Salvius²,

Joseph³

et al. 2020

GSC Adv. Res. Rev.

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Dalbergia katangensis is used in traditional medicine in Bukavu in management of malaria. In this study, antiplasmodial, antioxidant activities, and toxicological studies where carried out on aqueous and methanolic extracts of its leaves. The plant was selected following an ethnobotanical survey conducted in DR Congo and focusing on plants used traditionally to treat malaria. Extrac's phytochemical secondary metabolites were determined using standard procedures and the antiplasmodial activity was evaluated using 4-day suppressive test, while antioxidant activity was evaluated by DPPH assay. In acute toxicity, twenty animals (5/group) were given orally singular 2000 mg of extract/kg body weight (BW) then observed for 14 days. In sub-acute toxicity assay, 200, 400 mg/kg BW/Day were given orally, and animals were observed for 28 days. The total phenolic (0.58-0.61 mg GAEg -1 ), total flavonoid (0.14-0.15 mg QEg -1 ) and total terpenoids contents (0.32-0.55 %w/w) were in the same rate in the two extracts as well as the antioxidant activity with IC50 value 1.55 ± 0.012 and 1.71 ± 0.014 μg/mL. At the highest oral dose,400 mg/kg body weight, all extracts produced 73-75% chemo-suppression against P. berghei ANKA and 28 survival days. No deaths were recorded during the acute toxicity assay suggesting the LD50 >2000 mg/kg and no abnormal behavior or variation in toxicity biomarkers were observed during the subacute toxicity assessment. Dalbergia katangensis leaves extracts showed a great antiplasmodial and a very good antioxidant activity. It can be used to prepare antimalarial recipe or isolate antimalarial compounds in the future.

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Antimalarial Activity and Toxicological Assessment of Betula alnoides Extract against Plasmodium berghei Infections in Mice

Cited by 25 publications

References 17 publications

In VitroandIn VivoAntimalarial Activities and Toxicological Assessment ofPogostemon Cablin(Blanco) Benth

In VitroandIn VivoAntimalarial Activities and Toxicological Assessment ofPogostemon Cablin(Blanco) Benth

The Antioxidant Activities of Betula etnensis Rafin. Ethanolic Extract Exert Protective and Anti-diabetic Effects on Streptozotocin-Induced Diabetes in Rats

Antiplasmodial, antioxidant and toxicological study of leaves extracts of Dalbergia katangensis Lecheneaud (Fabaceae) from Eastern DR Congo

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