Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract

Lee, Jeong-Oog; Kim, Eunji; Kim, Ji Hye; Hong, Yo Han; Kim, Han Gyung; Jeong, Deok; Kim, Juewon; Kim, Su Hwan; Park, Chan-Woong; Seo, Dae Bang; Son, Young–Jin; Han, Sang Yun; Cho, Jae Youl

doi:10.1016/j.jgr.2018.02.007

Cited by 51 publications

(30 citation statements)

References 48 publications

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“…This process is characterized by formation of coarse wrinkles, thickening of skin, and dryness [7][8][9][10]. In melanocytes, ROS regulate melanogenesis [11,12], and UVB irradiation stimulates keratinocytes to induce α-melanocyte-stimulating hormone (α-MSH) secretion, which triggers cell signaling in response to melanocortin 1 receptor (MC1R) of melanocytes [6]. In melanogenesis, the tyrosinase protein is regulated by the MC1R signaling pathway.…”

Section: Introductionmentioning

confidence: 99%

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

Lim

Jeong

Park

et al. 2020

IJMS

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UVB irradiation can induce generation of reactive oxygen species (ROS) that cause skin aging or pigmentation. Lactobacillus acidophilus is a well-known probiotic strain that regulates skin health through antimicrobial peptides and organic products produced by metabolism and through immune responses. In this study, we investigated the antioxidative, antiwrinkle, and antimelanogenesis effects of tyndallized Lactobacillus acidophilus KCCM12625P (AL). To analyze the effects of AL on UV irradiation-induced skin wrinkle formation in vitro, human keratinocytes and human dermal fibroblasts were exposed to UVB. Subsequent treatment with AL induced antiwrinkle effects by regulating wrinkle-related genes such as matrix metalloproteinases (MMPs), SIRT-1, and type 1 procollagen (COL1AL). In addition, Western blotting assays confirmed that regulation of MMPs by AL in keratinocytes was due to regulation of the AP-1 signaling pathway. Furthermore, we confirmed the ability of AL to regulate melanogenesis in B16F10 murine melanoma cells treated with α-melanocyte-stimulating hormone (α-MSH). In particular, AL reduced the mRNA expression of melanogenesis-related genes such as tyrosinase, TYRP-1, and TYRP-2. Finally, we used Western blotting assays to confirm that the antimelanogenesis role of AL was due to its regulation of the cyclic adenosine monophosphate (cAMP) signaling pathway. Collectively, these results indicate that AL has an antiwrinkle activity in damaged skin and can inhibit melanogenesis. Thus, AL should be considered an important substance for potential use in anti-aging drugs or cosmetics.

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Section: Introductionmentioning

confidence: 99%

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

Lim

Jeong

Park

et al. 2020

IJMS

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“…Before UVB irradiation, culture medium was replaced with 1 ml of phosphate-buffered saline (PBS) per well. After removing the plate lid, cells were irradiated at 30 mJ/cm 2 [25]. After UVB irradiation, PBS was replaced with complete culture medium with the appropriate compound treatments prior to harvesting.…”

Section: Cell Viability Assaymentioning

confidence: 99%

Photoaging Protective Effects of Ranunculus bulumei Methanol Extract

Hong

Kim

Cho

2020

Evidence-Based Complementary and Alternative Medicine

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Ultraviolet B (UVB) radiation is the main cause of photoaging processes including cellular senescence, skin drying, collagen degradation, melanogenesis, and inflammation. These responses occur because UVB induces a change in expression of aging-related genes through regulation of signal pathways such as that of mitogen-activated protein kinases- (MAPKs-) activator protein 1 (AP-1). Ranunculus bulumei, which is used as an herb in Indonesia, belongs to the Ranunculaceae family, which has been reported to perform various physiological effects including antioxidant and anti-inflammation. However, data on the pharmaceutical and cosmeceutical utility of Ranunculus bulumei have not been reported. Therefore, we evaluated the antiaging efficacy of RB-ME, a methanol extract of Ranunculus bulumei. Rb-ME attenuated MMP9 and COX-2 gene expression but enhanced SIRT1 and type-1 collagen in UVB-irradiated HaCaT cells. Rb-ME regulated these gene expressions through inhibition of p38 phosphorylation and inactivation of AP-1. In addition, mRNA expression of HAS-2 and -3, which are involved in skin hydration, was elevated in Rb-ME-treated HaCaT cells. Rb-ME also inhibited melanogenesis by suppression of tyrosinase, MITF, and TYRP-1 mRNA in B16F10 cells under α-MSH treatment. Taken together, these results indicate that Rb-ME has a protective effect on some UVB-induced skin photoaging events such as inflammation, collagen degradation, cellular senescence, skin drying, and melanin production through inhibition of the p38-AP-1 signal cascade, indicating that Rb-ME can be used as an active ingredient for antiaging cosmetics.

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“…The nuclear fraction was prepared as described previously [25]. Immunoblotting of both nuclear fraction and whole-cell lysate were performed as previously described [24,26]. β-actin and lamin A/C were used as a loading control for the whole cell lysate and nuclear fraction, respectively.…”

Section: Preparation Of Whole-cell Lysates Nuclear Fraction and Immmentioning

confidence: 99%

Regulation of 8-Hydroxydaidzein in IRF3-Mediated Gene Expression in LPS-Stimulated Murine Macrophages

et al. 2020

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Cytokines and chemokines are transcriptionally regulated by inflammatory transcription factors such as nuclear factor-κB (NF-κB), activator protein-1 (AP-1), and interferon regulatory factor (IRF)-3. A daidzein derivative compound, 8-hydroxydaidzein (8-HD), isolated from soy products, has recently gained attention due to various pharmacological benefits, including anti-inflammatory activities. However, regulation of the inflammatory signaling mechanism for 8-HD is still poorly understood, particularly with respect to the IRF-3 signaling pathway. In this study, we explored the molecular mechanism of 8-HD in regulating inflammatory processes, with a focus on the IRF-3 signaling pathway using a lipopolysaccharide (LPS) and polyinosinic:polycytidylic acid [Poly (I:C)] stimulated murine macrophage cell line (RAW264.7). The 8-HD downregulated the mRNA expression level of IRF-3-dependent genes by inhibiting phosphorylation of the IRF-3 transcription factor. The inhibitory mechanism of 8-HD in the IRF-3 signaling pathway was shown to inhibit the kinase activity of IKKε to phosphorylate IRF-3. This compound can also interfere with the TRIF-mediated complex formation composed of TRAF3, TANK, and IKKε leading to downregulation of AKT phosphorylation and reduction of IRF-3 activation, resulted in inhibition of IRF-3-dependent expression of genes including IFN-β, C-X-C motif chemokine 10 (CXCL10), and interferon-induced protein with tetratricopeptide repeats 1 (IFIT1). Therefore, these results strongly suggest that 8-HD can act as a promising compound with the regulatory function of IRF-3-mediated inflammatory responses.

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Antimelanogenesis and skin-protective activities of Panax ginseng calyx ethanol extract

Cited by 51 publications

References 48 publications

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

Antiwrinkle and Antimelanogenesis Effects of Tyndallized Lactobacillus acidophilus KCCM12625P

Photoaging Protective Effects of Ranunculus bulumei Methanol Extract

Regulation of 8-Hydroxydaidzein in IRF3-Mediated Gene Expression in LPS-Stimulated Murine Macrophages

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