Antimicrobial action of an endophytic fungi from<i>Sophor flavescens</i>and structure identification of its active constituent

Yu, Na; He, Liang; Liu, Na; Wang, Yong; Xu, Hongbo; Liu, Dandan

doi:10.1080/13102818.2014.911618

Cited by 6 publications

(5 citation statements)

References 16 publications

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“…In this study, for the first time, the plant growth-promoting activities of endophytes isolated from various organs of S. flavescens (shrubby sophora) were analyzed in order to compare and evaluate their effects on root growth, biomass and the photosynthetic pigments (chlorophyll-a, -b and carotenoids) in A. thaliana. Previous studies on fungal endophytes inhabiting the species S. flavescens comprised only two fungal genera, Aspergillus and Penicillium [14][15][16]. Based on our examinations, the fungal isolates represented the genera of Alternaria, Didymella, Fusarium and Xylogone, which have not been previously reported as fungal endophytes of S. flavescens.…”

Section: Discussionmentioning

confidence: 50%

“…Furthermore, endophytic fungi Aspergillus terreus and a Penicillium sp. were isolated from S. flavescens and their bioactive compounds were purified and identified [14,15]. According to Zhang et al, the A. terreus strain was also isolated from the seeds of this plant and the fungal production of a host metabolite was confirmed and optimized [16].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

et al. 2020

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Endophytic fungi in symbiotic association with their host plant are well known to improve plant growth and reduce the adverse effects of both biotic and abiotic stresses. Therefore, fungal endophytes are beginning to receive increased attention in an effort to find growth-promoting strains that could be applied to enhance crop yield and quality. In our study, the plant growth-promoting activities of endophytic fungi isolated from various parts of Sophora flavescens (a medicinally important plant in Mongolia and China) have been revealed and investigated. Fungal isolates were identified using molecular taxonomical methods, while their plant growth-promoting abilities were evaluated in plate assays. Altogether, 15 strains were isolated, representing the genera Alternaria, Didymella, Fusarium and Xylogone. Five of the isolates possessed phosphate solubilization activities and twelve secreted siderophores, while all of them were able to produce indoleacetic acid (IAA) in the presence or absence of tryptophan. The endogenous and exogenous accumulation of IAA were also monitored in liquid cultures using the HPLC-MS/MS technique to refine the plate assay results. Furthermore, for the highest IAA producer fungi, the effects of their extracts were also examined in plant bioassays. In these tests, the primary root lengths of the model Arabidopsis thaliana were increased in several cases, while the biomasses were significantly lower than the control IAA treatment. Significant alterations have also been detected in the photosynthetic pigment (chlorophyll-a, -b and carotenoids) content due to the fungal extract treatments, but these changes did not show any specific trends.

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Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

et al. 2020

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“…The PCR product was sent to Shanghai Sangon Biological Engineering Technology & Services Co., Ltd., for sequence determination. The sequence was compared to those of other related species downloaded from a public database using the EzBioCloud tool, and clustering analysis and phylogenetic tree construction using the Neighbor-Joining method were performed using MEGA 5.1 software [ 27 – 28 ].…”

Section: Methodsmentioning

confidence: 99%

Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere

et al. 2018

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Streptomyces species 1–14 isolated from cassava rhizosphere soil were evaluated for their antibacterial efficacy against Fusarium oxysporum f.sp. cubense race 4 (FOC4). Of the 63 strains tested, thirteen exhibited potent antibacterial properties and were further screened against eight fungal pathogens. The strain that showed maximum inhibition against all of the test pathogens was identified by 16S rDNA sequencing as Streptomyces sp. 1–14, was selected for further studies. Through the propagation of Streptomyces sp. 1–14 in soil under simulated conditions, we found that FOC4 did not significantly influence the multiplication and survival of Streptomyces sp. 1–14, while indigenous microorganisms in the soil did significantly influence Streptomyces sp. 1–14 populations. To achieve maximum metabolite production, the growth of Streptomyces 1–14 was optimized through response surface methodology employing Plackett-Burman design, path of steepest ascent determinations and Box-Behnken design. The final optimized fermentation conditions (g/L) included: glucose, 38.877; CaCl2•2H2O, 0.161; temperature, 29.97°C; and inoculation amount, 8.93%. This optimization resulted in an antibacterial activity of 56.13% against FOC4, which was 12.33% higher than that before optimization (43.80%). The results obtained using response surface methodology to optimize the fermentation medium had a significant effect on the production of bioactive metabolites by Streptomyces sp. 1–14. Moreover, during fermentation and storage, pH, light, storage temperature, etc., must be closely monitored to reduce the formation of fermentation products with reduced antibacterial activity. This method is useful for further investigations of the production of anti-FOC4 substances, and could be used to develop bio-control agents to suppress or control banana fusarium wilt.

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“…Endophytes produce bioactive compounds similar to those in the host plant (Yu et al, 2014). In addition, novel chemical skeletons of secondary metabolites have antimicrobial, antiparasitic, cytotoxic, anti-inflammatory, antitumor, antioxidant and neuroprotective activities (Malhadas et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of secondary metabolites produced by Aspergillus neobridgeri isolated from Pistacia lentiscus against multi-drug resistant bacteria

Sadrati¹,

Zerroug²,

Demirel³

et al. 2020

Bangladesh J Pharmacol

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The in vitro antibacterial activity of the endophytic fungus Aspergillus neobridgeri PLR9 (isolated from the plant Pistacia lentiscus) against a wide range of tested bacteria were studied. The maximum inhibitory activity was found on malt extract agar medium with a mean inhibitory zone of 11.3 mm (p<0.05). The ethyl acetate extract showed a significant inhibitory zone of 13.4 mm (p<0.05), followed by dichloromethane 9.1 mm. Whereas, n-hexane extract showed no activity. The largest inhibition zones were observed against Staphylococcus aureus 3, S. aureus 5 and Escherichia coli 1 with 21.0 mm of diameter. The MIC's were ranged between 312.5 and 1,250 μg/mL. The MBC's were ≤2500 μg/mL for most Gram positive bacteria and ≥2500 μg/mL for most Gram negative bacteria. Time kill assay study against the Enterococcus faecium showed a bactericidal effect at 4x MIC and bacteriostatic against Acinetobacter baumannii at different concentrations. Moreover, it is the first report, PLR9 isolated as endophyte and studied for their antimicrobial activity.

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Antimicrobial action of an endophytic fungi fromSophor flavescensand structure identification of its active constituent

Cited by 6 publications

References 16 publications

Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

Characterization of the Plant Growth-Promoting Activities of Endophytic Fungi Isolated from Sophora flavescens

Optimization of fermentation conditions through response surface methodology for enhanced antibacterial metabolite production by Streptomyces sp. 1-14 from cassava rhizosphere

Antimicrobial activity of secondary metabolites produced by Aspergillus neobridgeri isolated from Pistacia lentiscus against multi-drug resistant bacteria

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