Antimicrobial activities of components of the glandular secretions of leaf cutting ants of the genus Atta

Mendonça, Adriana de Lima; Silva, Carlos Eduardo da; Mesquita, Fernando Lucas Torres de; Campos, Rousseau da Silva; Nascimento, Ruth R. Do; Ximenes, Eulália Camelo Pessoa de Azevedo; Santana, Antônio Euzébio Goulart

doi:10.1007/s10482-009-9312-0

Cited by 49 publications

(27 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…M. anisopliae and M. acridum are predicted to contain four and two CYP504s, respectively. CYP504s are used by fungi to degrade phenylacetate [50], an antimicrobial compound found in plants and insects [51]. The subfamily CYP53 is also represented in the PHI database as it is responsible for detoxification of benzoate and its derivatives [52].…”

Section: Resultsmentioning

confidence: 99%

Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum

et al. 2011

View full text Add to dashboard Cite

Metarhizium spp. are being used as environmentally friendly alternatives to chemical insecticides, as model systems for studying insect-fungus interactions, and as a resource of genes for biotechnology. We present a comparative analysis of the genome sequences of the broad-spectrum insect pathogen Metarhizium anisopliae and the acridid-specific M. acridum. Whole-genome analyses indicate that the genome structures of these two species are highly syntenic and suggest that the genus Metarhizium evolved from plant endophytes or pathogens. Both M. anisopliae and M. acridum have a strikingly larger proportion of genes encoding secreted proteins than other fungi, while ∼30% of these have no functionally characterized homologs, suggesting hitherto unsuspected interactions between fungal pathogens and insects. The analysis of transposase genes provided evidence of repeat-induced point mutations occurring in M. acridum but not in M. anisopliae. With the help of pathogen-host interaction gene database, ∼16% of Metarhizium genes were identified that are similar to experimentally verified genes involved in pathogenicity in other fungi, particularly plant pathogens. However, relative to M. acridum, M. anisopliae has evolved with many expanded gene families of proteases, chitinases, cytochrome P450s, polyketide synthases, and nonribosomal peptide synthetases for cuticle-degradation, detoxification, and toxin biosynthesis that may facilitate its ability to adapt to heterogenous environments. Transcriptional analysis of both fungi during early infection processes provided further insights into the genes and pathways involved in infectivity and specificity. Of particular note, M. acridum transcribed distinct G-protein coupled receptors on cuticles from locusts (the natural hosts) and cockroaches, whereas M. anisopliae transcribed the same receptor on both hosts. This study will facilitate the identification of virulence genes and the development of improved biocontrol strains with customized properties.

show abstract

Section: Resultsmentioning

confidence: 99%

Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Both CYP53 and CYP504 family members were found to be upregulated during cuticle infection by insect pathogenic fungi Metarhizium anisopliae (four genes) and M. acridum (two genes) [50]. It was suggested that in these insect pathogens these CYP families carry out detoxification of insect released phenylacetate [50,51]. …”

Section: Resultsmentioning

confidence: 99%

Systematic and searchable classification of cytochrome P450 proteins encoded by fungal and oomycete genomes

et al. 2012

View full text Add to dashboard Cite

BackgroundCytochrome P450 proteins (CYPs) play diverse and pivotal roles in fungal metabolism and adaptation to specific ecological niches. Fungal genomes encode extremely variable “CYPomes” ranging from one to more than 300 CYPs. Despite the rapid growth of sequenced fungal and oomycete genomes and the resulting influx of predicted CYPs, the vast majority of CYPs remain functionally uncharacterized. To facilitate the curation and functional and evolutionary studies of CYPs, we previously developed Fungal Cytochrome P450 Database (FCPD), which included CYPs from 70 fungal and oomycete species. Here we present a new version of FCPD (1.2) with more data and an improved classification scheme.ResultsThe new database contains 22,940 CYPs from 213 species divided into 2,579 clusters and 115 clans. By optimizing the clustering pipeline, we were able to uncover 36 novel clans and to assign 153 orphan CYP families to specific clans. To augment their functional annotation, CYP clusters were mapped to David Nelson’s P450 databases, which archive a total of 12,500 manually curated CYPs. Additionally, over 150 clusters were functionally classified based on sequence similarity to experimentally characterized CYPs. Comparative analysis of fungal and oomycete CYPomes revealed cases of both extreme expansion and contraction. The most dramatic expansions in fungi were observed in clans CYP58 and CYP68 (Pezizomycotina), clans CYP5150 and CYP63 (Agaricomycotina), and family CYP509 (Mucoromycotina). Although much of the extraordinary diversity of the pan-fungal CYPome can be attributed to gene duplication and adaptive divergence, our analysis also suggests a few potential horizontal gene transfer events. Updated families and clans can be accessed through the new version of the FCPD database.ConclusionsFCPD version 1.2 provides a systematic and searchable catalogue of 9,550 fungal CYP sequences (292 families) encoded by 108 fungal species and 147 CYP sequences (9 families) encoded by five oomycete species. In comparison to the first version, it offers a more comprehensive clan classification, is fully compatible with Nelson’s P450 databases, and has expanded functional categorization. These features will facilitate functional annotation and classification of CYPs encoded by newly sequenced fungal and oomycete genomes. Additionally, the classification system will aid in studying the roles of CYPs in the evolution of fungal adaptation to specific ecological niches.

show abstract

“…Citronellol substantially increased the specific biofilm formation of E. coli ATCC 33456, possibly by acting as a poly L-lysine like adhesive 6 , but it decreased the specific biofilm formation of P. aeruginosa and P. putida at equivalent concentration ranges 20 . Also, geranial, β-citronellol were found in Pelargonium graveolens oil, geraniol and citronellol exhibited strong activity against C. albicans 16 but the antimicrobial activity of geraniol was more higher than β-citronellol, particularly against C. albicans 7 .…”

Section: Discussionmentioning

confidence: 95%

Chemical Composition and Antimicrobial Activity ofRosa damascenaMill Essential Oil

Mahboubi¹,

Kazempour²,

Khamechian

et al. 2011

Journal of Biologically Active Products from Nature

View full text Add to dashboard Cite

Rosa damascena Mill. a plant from Rosaceae family is traditionally used for treatment of many disorders especially against infectious diseases. The antimicrobial activity of R. damascena oil was determined against a large number of microorganisms including Gram positive and Gram negative bacteria, yeast and filamentous by micro broth dilution assay. The oil was analyzed by GC and GC-MS. Chemical composition of oil revealed the presence of β-citronellol (48.2 %), geraniol (17.0 %), β-phenylethyl benzoate (5.4 %) and phenyl ethyl alcohol (5.1 %) as the main components. The MIC values of oil were in the ranges of 0.125-1 μl/ml. Enterococcus faecalis, Enterococcus faecium, Salmonella typhimurium and Pseudomonas aeruginosa were less sensitive to R. damascena oil. The oil exhibited the antimicrobial activity against a large number of microorganisms especially against Proteus vulgaris and Klebsiella pneumonia. This oil could be used as a natural antimicrobial agent in treatment of infectious diseases; more clinical studies should be done for demonstration of its efficacy.

show abstract

Antimicrobial activities of components of the glandular secretions of leaf cutting ants of the genus Atta

Cited by 49 publications

References 39 publications

Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum

Genome Sequencing and Comparative Transcriptomics of the Model Entomopathogenic Fungi Metarhizium anisopliae and M. acridum

Systematic and searchable classification of cytochrome P450 proteins encoded by fungal and oomycete genomes

Chemical Composition and Antimicrobial Activity ofRosa damascenaMill Essential Oil

Contact Info

Product

Resources

About