Antimicrobial activities of endophytic fungi of the Sri Lankan aquatic plant<i>Nymphaea nouchali</i>and chaetoglobosin A and C, produced by the endophytic fungus<i>Chaetomium globosum</i>

Dissanayake, Ranga K.; Ratnaweera, Pamoda B.; Williams, David E.; Wijayarathne, C. Dilrukshi; Wijesundera, R.L.C.; Andersen, Raymond J.; Silva, E. Dilip de

doi:10.1080/21501203.2015.1136708

Cited by 49 publications

(17 citation statements)

References 16 publications

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“…Similar, standardized endophytic fungal isolation by imprint method has been used in Ficus pumila, Basella rubra and Camellia sinensis to evaluate the effectiveness of surface sterilization of the plant materials (Rakshith et al 2013;Hema et al 2015;Guo et al 2016). The results of the present study are in agreement with the findings of Rao et al (2015), where a single endophytic fungus Gliomastix polychrome was isolated from surface-sterilized bark of Combretum latifolium, while there are also reports of isolation of more than one endophytic fungi from Nymphaea nouchali (Dissanayake et al 2016). Our fungal isolate emerging from the tissue of the inoculated plant part was identified as Aspergillus sp.…”

Section: Isolation and Identification Of Endophytic Fungisupporting

confidence: 83%

“…Likewise, ethyl acetate extract of endophytic fungi from Bacopa monnieri displayed antimicrobial activity against B. subtilis, P. aeruginosa, S. typhimurium, E. coli, K. pneumonia and S. aureus with MIC of 10-100 lg/mL (Katoch et al 2014). Also, Ratnaweera et al (2015) and Dissanayake et al (2016) have reported that endophytic fungi Fusarium sp. and Chaetomium globosum isolated from Opuntia dillenii and N. nouchali, respectively, offered antibacterial activity with MIC ranging from 8 to 32 lg/mL against B. subtilis, S. aureus and methicillin-resistant S. aureus.…”

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

“…have shown parallel results against B. subtilis, E. coli, Micrococcus luteus, Pseudomonas aeruginosa and S. aureus. Likewise, Rao et al (2015) and Dissanayake et al (2016) reported significant antibacterial potential of endophytic fungus isolated from Combretum latifolium and Nymphaea nouchali, respectively.…”

Section: Antibacterial Activitymentioning

confidence: 99%

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Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Murali

Mahendra

Hema

et al. 2017

Pharmaceutical Biology

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Context: Sida acuta Burm.f. (Malvaceae) extracts are reported to have applications against malaria, diuretic, antipyretic, nervous and urinary diseases. No fungal endophytes of S. acuta are reported. Objective: Isolation, identification and evaluation of antibacterial, antioxidant, anticancer and haemolytic potential of fungal endophytes from the ethnomedcinal plant S. acuta. Materials and methods: Sida acuta stem segments were placed on PDA medium to isolate endophytic fungi. The fungus was identified by genomic DNA analysis and phylogenetic tree was constructed using ITS sequences (GenBank) to confirm species. The antibacterial efficacy of Aspergillus sulphureus MME12 ethyl acetate extract was tested against Gram-positive and Gram-negative pathogenic bacteria. DPPH free radical scavenging activity, anticancer and DNA fragmentation against EAC cells, and direct haemolytic activity (100-500 lg/mL) using human erythrocytes were determined. Results and discussion: The ethyl acetate extract of A. sulphureus (Fresen.) Wehmer (Trichocomaceae) demonstrated significant antibacterial potential against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi compared to streptomycin. MIC against test pathogens was in the range of 15.6-62.5 lg/mL. The antioxidant results revealed significant RSA from 12.43% to 62.02% (IC 50 ¼ 350.4 lg/ mL, p 0.05). MME12 offered considerable inhibition of EAC proliferation (23% to 84%, IC 50 ¼ 216.7 lg/mL, p 0.05) supported by DNA fragmentation studies. The extract also offered insignificant haemolysis (5.6%) compared to Triton X-100. Conclusions: A single endophytic fungus, A. sulphureus MME12 was isolated and identified using molecular profiling. The above-mentioned findings support the pharmacological application of A. sulphureus MME12 extract and demand for purification of the active principle(s).

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Section: Isolation and Identification Of Endophytic Fungisupporting

confidence: 83%

Section: Minimum Inhibitory Concentration (Mic)mentioning

confidence: 99%

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Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Murali

Mahendra

Hema

et al. 2017

Pharmaceutical Biology

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“…The fungal strain was cultured in PDA at a room temperature for 3 days. Mycelium of fungus was scraped and the isolation of genomic DNA from the mycelia of fungal isolate was carried out according to the published protocol as previously reported ( Dissanayake et al, 2016 ). The ITS region of the isolated genomic DNA was amplified by polymerase chain reaction (PCR) using the forward primer ITS1 and the reverse primer ITS4 under the conditions.…”

Section: Methodsmentioning

confidence: 99%

Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera

Techaoei

Jirayuthcharoenkul

Jarmkom

et al. 2020

Saudi Journal of Biological Sciences

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The objective of this study was to characterize an endophytic fungi producing-bioactive compound from the aquatic plant, Nelumbo nucifera . All parts of such plant were cleaned with surface sterilization technique and cultured on potato dextrose agar to isolate endophytic fungi. The identification was characterized by morphological and molecular technique. Fungal isolates were screened to discover antimicrobial activities by disc diffusion method against Methicillin-resistant Staphylococcus aureus DMST20651 (MRSA). MIC and MBC for those crude fungal extracts were determined. Finally, the chemical profile of crude extract was determined by gas chromatography and mass spectrometry. Six endophytic fungi were isolated from the surface-satirized parts of N. nucifera . Based on disc diffusion assay, the highest antibacterial activity against MRSA was isolate ST9.1 identified as Aspergillus cejpii . Results demonstrated that the ethyl acetate extraction had more active fractions with MIC of 2.5 mg/ml and MBC concentration of 50.0 mg/ml. The crude extracts were developed to identify the chemical constituents by gas chromatography and mass spectrometry. The major component of crude extract of endophytic fungi was 5-(1H-Indol-3-yl)-4,5-dihydro-[1,2,4]triazin-3-ylamine (C 11 H 11 N 5 ). Thus, the plant could be used in the treatment of infectious diseases caused by bacterial pathogen.

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“…An endophytic fungus of Nymphaea nouchali (Sri Lanka) called Chaetomium globosum, led to the isolation of cytochalasans, chaetoglobosin A and C from the crude extract of the two distinct fungi. The compounds showed antibacterial activities against test microorganisms, and chaetoglobosin A (1) inhibited MRSA ATCC 33591 and S. aureus ATCC 43300 at MIC values of 32 and 32 µg/mL, respectively [88].…”

Section: Marine Alga-associated Fungimentioning

confidence: 98%

Antibiotics Development and the Potentials of Marine-Derived Compounds to Stem the Tide of Multidrug-Resistant Pathogenic Bacteria, Fungi, and Protozoa

et al. 2020

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As the search for new antibiotics continues, the resistance to known antimicrobial compounds continues to increase. Many researchers around the world, in response to antibiotics resistance, have continued to search for new antimicrobial compounds in different ecological niches such as the marine environment. Marine habitats are one of the known and promising sources for bioactive compounds with antimicrobial potentials against currently drug-resistant strains of pathogenic microorganisms. For more than a decade, numerous antimicrobial compounds have been discovered from marine environments, with many more antimicrobials still being discovered every year. So far, only very few compounds are in preclinical and clinical trials. Research in marine natural products has resulted in the isolation and identification of numerous diverse and novel chemical compounds with potency against even drug-resistant pathogens. Some of these compounds, which mainly came from marine bacteria and fungi, have been classified into alkaloids, lactones, phenols, quinones, tannins, terpenes, glycosides, halogenated, polyketides, xanthones, macrocycles, peptides, and fatty acids. All these are geared towards discovering and isolating unique compounds with therapeutic potential, especially against multidrug-resistant pathogenic microorganisms. In this review, we tried to summarize published articles from 2015 to 2019 on antimicrobial compounds isolated from marine sources, including some of their chemical structures and tests performed against drug-resistant pathogens.

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Antimicrobial activities of endophytic fungi of the Sri Lankan aquatic plantNymphaea nouchaliand chaetoglobosin A and C, produced by the endophytic fungusChaetomium globosum

Cited by 49 publications

References 16 publications

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera

Antibiotics Development and the Potentials of Marine-Derived Compounds to Stem the Tide of Multidrug-Resistant Pathogenic Bacteria, Fungi, and Protozoa

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