Chemical evaluation and antibacterial activity of novel bioactive compounds from endophytic fungi in Nelumbo nucifera

Abstract

The objective of this study was to characterize an endophytic fungi producing-bioactive compound from the aquatic plant, Nelumbo nucifera . All parts of such plant were cleaned with surface sterilization technique and cultured on potato dextrose agar to isolate endophytic fungi. The identification was characterized by morphological and molecular technique. Fungal isolates were screened to discover antimicrobial activities by disc diffusion method against Methicillin-resistant Staphylococcus aureus DMST20651 (MRSA). MIC and MBC for those crude fungal extracts were determined. Finally, the chemical profile of crude extract was determined by gas chromatography and mass spectrometry. Six endophytic fungi were isolated from the surface-satirized parts of N. nucifera . Based on disc diffusion assay, the highest antibacterial activity against MRSA was isolate ST9.1 identified as Aspergillus cejpii . Results demonstrated that the ethyl acetate extraction had more active fractions with MIC of 2.5 mg/ml and MBC concentration of 50.0 mg/ml. The crude extracts were developed to identify the chemical constituents by gas chromatography and mass spectrometry. The major component of crude extract of endophytic fungi was 5-(1H-Indol-3-yl)-4,5-dihydro-[1,2,4]triazin-3-ylamine (C 11 H 11 N 5 ). Thus, the plant could be used in the treatment of infectious diseases caused by bacterial pathogen.

Metabolites of medicine food homology-derived endophytic fungi and their activities

“…Endophytic fungi were treated with different concentrations of valproic acid to obtain extracts ( 106 ). In another study, ethyl acetate extract of endophyte Aspergillus cejpii ST9.1 ( 107 ) was isolated from the aquatic plant Nelumbo nucifera ; the major component of the crude extract was 5-(1H-Indol-3-yl)-4,5-dihydro-[1,2,4]triazin-3-ylamine (C 11 H 11 N 5 ) ( Techaoei et al, 2020 ). Three strains of Fusarium species (J-1, J-2, and J-3) have been isolated from Ginkgo biloba , yielding a crude extract ( 108 ) ( Engel, 2019 ).…”

“…Valproic acid-treated fungal extracts ( 106 ) showed significant antibacterial efficiency against the bacteria Pseudomonas aeruginosa , Shigella sonnei , and Streptococcus pyogenes ( Ameen et al, 2021 ). An ST9.1-EtOAc extract ( 107 ) had active fractions against methicillin-resistant Staphylococcus aureus (MRSA) with a MIC of 2.5 mg/mL and minimum bactericidal concentration (MBC) concentration of 50.0 mg/mL ( Techaoei et al, 2020 ). An AP5-EtOAc extract ( 112 ) displayed pronounced antimicrobial properties against S .…”

Screening of Antibacterial Activities of the Endophytic Fungi Isolated from the Leaves of Medinilla speciosa Blume

“…All the bacterial tests were inoculated into the nutrient broth (NB) media and incubated at 35 °C -37 °C for 18-24 hours. Each of these bacterial suspensions was compared to McFarland standard 0.5 (1.5 x 108 CFU/ml) (Techaoei et al, 2020) for density uniformity and then 100 µl of the bacterial suspension was spread onto nutrient agar (NA) using a sterile cotton bud in a Petri dish until all surface was covered. Then, a filter paper discs (about 6 mm in diameter), containing 20 µl of the test compound are placed on the agar surface.…”

Combination on endophytic fungal as the Plant Growth-Promoting Fungi (PGPF) on cucumber (Cucumis sativus)

“…These fungi play an important role in the ecosystem as well as plant growth (Zheng et al 2017;Afandhi et al 2018), produce bioactive molecules (Spellberg et al 2008;Vasundhara et al 2016;Manganyi et al 2019;Techaoei et al 2020), phytohormones (IAA, gibberellins, and cytokinins) (Lu et al 2000; Barka et al 2002) (Wulandari and Suryantini 2019), as well as siderophore (Neilands 1952;Ghosh et al 2017), and also act as phosphate solvent (Malinowski and Belesky 1999;Hamayun et al 2011).…”