Antimicrobial activities of some Streptomyces isolated from garden soil samples and fish pond water in Futa

Ekundayo, F. O.; Oyeniran, Kehinde Adewole; Adedokun, AD

doi:10.3329/jbs.v22i0.30005

Cited by 2 publications

(2 citation statements)

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“…Streptomyces species isolates were evaluated for their antimicrobial activity toward the tested bacteria and fungi isolates using the agar plug method ( Ekundayo et al., 2014 ). Briefly, an inoculum (1.5× 10 8 CFU/mL) of fresh bacterial or fungal culture was inoculated on the surface of Muller-Hinton agar (MHA) plates or Sabouraud dextrose agar (Thermo Fisher Scientific Oxoid Ltd., Basingstoke, Hampshire, United Kingdom), and then Streptomyces colonies that had been cut with a sterile cork borer (8 mm) were deposited.…”

Section: Methodsmentioning

confidence: 99%

Bioactive metabolites of Streptomyces misakiensis display broad-spectrum antimicrobial activity against multidrug-resistant bacteria and fungi

Abdelaziz

Tartor

Barakat

et al. 2023

Front. Cell. Infect. Microbiol.

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BackgroundAntimicrobial resistance is a serious threat to public health globally. It is a slower-moving pandemic than COVID-19, so we are fast running out of treatment options.PurposeThus, this study was designed to search for an alternative biomaterial with broad-spectrum activity for the treatment of multidrug-resistant (MDR) bacterial and fungal pathogen-related infections.MethodsWe isolated Streptomyces species from soil samples and identified the most active strains with antimicrobial activity. The culture filtrates of active species were purified, and the bioactive metabolite extracts were identified by thin-layer chromatography (TLC), preparative high-performance liquid chromatography (HPLC), nuclear magnetic resonance (NMR) spectroscopy, and gas chromatography-mass spectrometry (GC-MS). The minimum inhibitory concentrations (MICs) of the bioactive metabolites against MDR bacteria and fungi were determined using the broth microdilution method.ResultsPreliminary screening revealed that Streptomyces misakiensis and S. coeruleorubidus exhibited antimicrobial potential. The MIC50 and MIC90 of S. misakiensis antibacterial bioactive metabolite (ursolic acid methyl ester) and antifungal metabolite (tetradecamethylcycloheptasiloxane) against all tested bacteria and fungi were 0.5 μg/ml and 1 μg/mL, respectively, versus S. coeruleorubidus metabolites: thiocarbamic acid, N,N-dimethyl, S-1,3-diphenyl-2-butenyl ester against bacteria (MIC50: 2 μg/ml and MIC90: 4 μg/mL) and fungi (MIC50: 4 μg/ml and MIC90: 8 μg/mL). Ursolic acid methyl ester was active against ciprofloxacin-resistant strains of Streptococcus pyogenes, S. agalactiae, Escherichia coli, Klebsiella pneumoniae, and Salmonella enterica serovars, colistin-resistant Aeromonas hydrophila and K. pneumoniae, and vancomycin-resistant Staphylococcus aureus. Tetradecamethylcycloheptasiloxane was active against azole- and amphotericin B-resistant Candida albicans, Cryptococcus neoformans, C. gattii, Aspergillus flavus, A. niger, and A. fumigatus. Ursolic acid methyl ester was applied in vivo for treating S. aureus septicemia and K. pneumoniae pneumonia models in mice. In the septicemia model, the ursolic acid methyl ester-treated group had a significant 4.00 and 3.98 log CFU/g decrease (P < 0.05) in liver and spleen tissue compared to the infected, untreated control group. Lung tissue in the pneumonia model showed a 2.20 log CFU/g significant decrease in the ursolic acid methyl ester-treated group in comparison to the control group. The haematological and biochemical markers in the ursolic acid methyl ester-treated group did not change in a statistically significant way. Moreover, no abnormalities were found in the histopathology of the liver, kidneys, lungs, and spleen of ursolic acid methyl ester-treated mice in comparison with the control group. ConclusionS. misakiensis metabolite extracts are broad-spectrum antimicrobial biomaterials that can be further investigated for the potential against MDR pathogen infections. Hence, it opens up new horizons for exploring alternative drugs for current and reemerging diseases.

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Section: Methodsmentioning

confidence: 99%

Bioactive metabolites of Streptomyces misakiensis display broad-spectrum antimicrobial activity against multidrug-resistant bacteria and fungi

Abdelaziz

Tartor

Barakat

et al. 2023

Front. Cell. Infect. Microbiol.

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“…The biological agent Streptomyces sp. is an actinobacterium that can produce bioactive compounds containing antibiotics, antiparasitics, and antifungals (Ekundayo et al, 2014). According to Purnomo et al, (2017),Streptomyces sp.…”

Section: Flowering Period and Weight Of Fruits Per Plant Periodmentioning

confidence: 99%

Application of Streptomyces sp. and Trichoderma sp. for Promoting Generative Plants Growth of Cherry Tomato (Lycopersicum cerasiformae Mill.)

et al. 2022

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Production of cherry tomatoes in Indonesia is still low, which might be due to the inappropriate planting and maintenance processes. This research applied biological agent microorganisms Streptomyces sp. and Trichoderma sp. as Plant Growth Promoting Microorganisms (PGPM) in sustainable agricultural systems. This study aimed to determine the effect of the concentration of microorganisms Streptomyces sp. and Trichoderma sp. on the growth and production of cherry tomato plants on the polybag scale. The study was arranged with different concentrations of microorganisms Streptomyces sp. and Trichoderma sp. These concentration applied consisted of 1 : 0 ; 0 : 1 ; 2: 2 ; 3 ; 1 and without PGPM, each repeated four times. The results showed that the treatment of PGPM Streptomyces sp. and without Trichoderma sp. (1:0) resulted in the shortest flowering period (33.99 days after planting). Meanwhile, the treatment without Streptomyces sp. and Trichoderma sp. (0 : 1) produced the highest solid weight fruit (69.82 grams/plant).

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Antimicrobial activities of some Streptomyces isolated from garden soil samples and fish pond water in Futa

Abstract: Context: Streptomyces are potential sources for secondary metabolites possessing a variety of biological activities with antimicrobial activity, which is used for human and animal treatment. It is estimated these bacteria synthesize more than 7,000 metabolites.

Cited by 2 publications

References 14 publications

Bioactive metabolites of Streptomyces misakiensis display broad-spectrum antimicrobial activity against multidrug-resistant bacteria and fungi

Bioactive metabolites of Streptomyces misakiensis display broad-spectrum antimicrobial activity against multidrug-resistant bacteria and fungi

Application of Streptomyces sp. and Trichoderma sp. for Promoting Generative Plants Growth of Cherry Tomato (Lycopersicum cerasiformae Mill.)

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