Phyllanthus amarus belonging to family Euphorbiaceae is an important medicinal plant from ancient times which is used in Chinese and Ayurvedic medicine. It is a small annual herb mostly found in Central and Southern India. All parts of this plant have medicinal value and are used in traditional medicines for curing various diseases such as jaundice, hepatitis B, C, microbial infections, viral diseases, tumors, and kidney stones. Keeping in view its immense medicinal potential, phytochemical analysis was performed using different solvents such as ethyl acetate, dimethylformamide, chloroform, dichloromethane, and n-Hexane. All the plant parts, viz., root, stem and leaf were separately analyzed for the detection of phenols and flavonoids, of which leaf exhibited the highest concentration compared to root and stem. Maximum percentage of phenols and flavonoids could be detected with dimethylformamide in comparison to other solvents that were used in the study. Dimethylformamide leaf extract displayed highest phenolic content of 40.75 mg/g and the highest flavonoid content of 51.5 mg/g.