Antimicrobial activity and protective properties of vaginal lactobacilli from healthy Bulgarian women

Dimitonova, Silviya Petrova; Danova, Svetla; Serkedjieva, Julia; Bakalov, B

doi:10.1016/j.anaerobe.2007.08.003

Cited by 43 publications

(42 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Therefore, this in vitro characterized defensive mechanism, can be responsible for stable domination of lactobacilli in healthy vaginal ecosystem. The active strains were identified as L. fermentum, L. gasseri and L. salivarius species, with some probiotic characteristics (8).…”

Section: Initial Characterization Of Produced Antimicrobial Metabolitesmentioning

confidence: 99%

See 1 more Smart Citation

Inhibitory Activity of Vaginal Lactobacilli Against Human Pathogens

Petrova

Georgieva

Dimitonova

et al. 2009

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Lactic acid bacteria have long been considered as protective microflora, able to displace and kill different pathogens. Twenty vaginal strains, pre-selected as probiotics, were additionally characterized as effective antagonists against human pathogens. Four clinical reference strains-Staphylococcus aureus ATCC 25923, Staphylococcus aureus (MRSA) ATCC 39592, Pseudomonas aeruginosa ATCC 27853 and E. coli ATCC 25922 were used as test-cultures in initial screening procedure. The active strains belonging to the species L. fermentum, L. gasseri and L. salivarius were tested against 10 antibiotic-resistant and problematic clinical isolates. In vitro the growth inhibition of two Acinetobacter baumannii and two Pseudomonas aeruginosa out-patient strains was observed. The active antimicrobials were the lactic acid and hydrogen peroxid produced during the cultivation of ten vaginal strains, in combination with termo and proteinase-sensitive bacteriocin-like metabolites. Our results provide a promising base for further characterization of active compounds synthesized from Bulgarian vaginal Lactobacillus strains.

show abstract

Section: Initial Characterization Of Produced Antimicrobial Metabolitesmentioning

confidence: 99%

“…Twenty vaginal lactobacilli, recently identified(8) were included in this study. They were isolated from vaginal swab samples from healthy Bulgarian volunteers at childbearing age.…”

mentioning

confidence: 99%

Inhibitory Activity of Vaginal Lactobacilli Against Human Pathogens

Petrova

Georgieva

Dimitonova

et al. 2009

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

show abstract

“…The well diffusion technique was used to detect the production of inhibitory substances in the supernatant fluid of the isolates (Schillinger & Lücke, 1987). In the agar well diffusion assay, overnight cultures of the indicator strains were used to inoculate appropriate agar growth media at 37 uC (Dimitonova et al, 2007). Wells of 5 mm diameter were cut into agar plates and 50 ml of filtered, cell-free supernatant fluid obtained from the third subculture of the micro-organisms grown in MRS broth was added to each well.…”

mentioning

confidence: 99%

Probiotic assessment of Enterococcus durans 6HL and Lactococcus lactis 2HL isolated from vaginal microflora

Nami

Abdullah

Haghshenas

et al. 2014

Journal of Medical Microbiology

View full text Add to dashboard Cite

Forty-five lactic acid bacteria (LAB) were isolated from the vaginal specimens of healthy fertile women, and the identities of the bacteria were confirmed by sequencing of their 16S rDNA genes. Among these bacteria, only four isolates were able to resist and survive in low pH, bile salts and simulated in vitro digestion conditions. Lactococcus lactis 2HL, Enterococcus durans 6HL, Lactobacillus acidophilus 36YL and Lactobacillus plantarum 5BL showed the best resistance to these conditions. These strains were evaluated further to assess their ability to adhere to human intestinal Caco-2 cells. Lactococcus lactis 2HL and E. durans 6HL were the most adherent strains. In vitro tests under neutralized pH proved the antimicrobial activity of both strains. Results revealed that the growth of Escherichia coli O26, Staphylococcus aureus and Shigella flexneri was suppressed by both LAB strains. The antibiotic susceptibility tests showed that these strains were sensitive to all nine antibiotics: vancomycin, tetracycline, ampicillin, penicillin, gentamicin, erythromycin, clindamycin, sulfamethoxazole and chloramphenicol. These data suggest that E. durans 6HL and Lactococcus lactis 2HL could be examined further for their useful properties and could be developed as new probiotics. INTRODUCTIONThe concept of eating or using live bacteria for health benefits goes back a hundred years (Metchnikoff, 1907). Lactic acid bacteria (LAB) are generally regarded as safe and constitute part of the probiotic concept, which has received significant attention over the past years (Metchnikoff, 1907;Salminen et al., 1998). Probiotics are live micro-organisms in the form of single or mixed cultures; when consumed at sufficient amounts, these probiotics provide beneficial health effects for hosts (Guarner et al., 2008;Lilly & Stillwell, 1965;Surawicz, 2003). Probiotics significantly affect the bioavailability of nutrients in the human body (Young & Huffman, 2003). These micro-organisms stimulate immune mechanisms and maintain normal human microbiota compositions; as a result, these micro-organisms reduce the risk of diarrhoea (Binns & Lee, 2010), antibiotic-related diarrhoea (Friedman, 2012), irritable bowel syndrome (Barouei et al., 2009;Parkes, 2010), inflammatory bowel disease (Geier et al., 2007;Gionchetti et al., 2002), vaginal infections (Reid & Bocking, 2003), atopic eczema (Bunselmeyer & Buddendick, 2010;Wickens et al., 2008), rheumatoid arthritis (Guarner et al., 2008) and cancers (Serban, 2014;Zhu et al., 2011). They are believed to be the dominant members of normal postpubertal and premenopausal vaginal microbiota (Nam et al., 2007). Clinical proof shows that the vaginal and urogenital floras play a dominant role in maintaining both protection from illnesses and the well-being of women (Burton et al., 2003 Liong, 2008).To obtain the ability to exert their probiotic effects, potential strains are expected to have desirable properties (Ouwehand et al., 1999; Zavisic et al., 2011). Probiotics have many selection criteria: human origin f...

show abstract

“…These probiotic bacteria produce antimicrobial bacteriocins, proteins that have been shown to inhibit urogenital pathogens [60]. Lactobacilli are also known to contribute lactic acid and hydrogen peroxide to the vaginal canal, which can prevent HIV by direct viral inhibition or indirectly by inhibiting pathogenic infections that increase host susceptibility to HIV acquisition [61-63]. In fact, pathogenic conditions in which lactobacilli are depleted from the vaginal lumen are accompanied by increased rates of HIV transmission, such as the microbial shift condition bacterial vaginosis, in which displacement of vaginal lactobacilli is accompanied by a 60% increased rate of HIV infection [64].…”

Section: Modification Of Naturally Occurring Anti-hiv Peptidesmentioning

confidence: 99%

Mechanisms and Modifications of Naturally Occurring Host Defense Peptides for Anti-HIV Microbicide Development

Eade¹,

Wood²,

Cole³

2012

CHR

View full text Add to dashboard Cite

Despite advances in the treatment of HIV infection, heterosexual transmission of HIV remains high, and vaccines to prevent HIV acquisition have been unfruitful. Vaginal microbicides, on the other hand, have demonstrated considerable potential for HIV prevention, and a variety of compounds have been screened for their activity and safety as anti-HIV microbicides. Among these are the naturally occurring host defense peptides, small peptides from diverse lineages with intrinsic antiviral activity. Naturally occurring host defense peptides with anti-HIV activity are promising candidates for vaginal microbicide development. Their structural variance and accompanying mechanistic diversity provide a wide range of inhibitors whose antiviral activity can be exerted at nearly every stage of the HIV lifecycle. Additionally, peptide modification has been explored as a method for improving the anti-HIV activity of host defense peptides. Structure- and sequence-based alterations have achieved varying success in improving the potency and specificity of anti-HIV peptides. Overall, peptides have been discovered or engineered to inhibit HIV with therapeutic indices of >1000, encouraging their advancement toward clinical trials. Here we review the naturally occurring anti-HIV host defense peptides, demonstrating their breadth of mechanistic diversity, and exploring approaches to enhance and optimize their activity in order to expedite their development as safe and effective anti-HIV vaginal microbicides.

show abstract

Antimicrobial activity and protective properties of vaginal lactobacilli from healthy Bulgarian women

Cited by 43 publications

References 28 publications

Inhibitory Activity of Vaginal Lactobacilli Against Human Pathogens

Inhibitory Activity of Vaginal Lactobacilli Against Human Pathogens

Probiotic assessment of Enterococcus durans 6HL and Lactococcus lactis 2HL isolated from vaginal microflora

Mechanisms and Modifications of Naturally Occurring Host Defense Peptides for Anti-HIV Microbicide Development

Contact Info

Product

Resources

About