Antimicrobial activity, cytotoxicity and DNA binding studies of carbon dots

Jhonsi, Mariadoss Asha; Ananth, Devanesan Arul; Nambirajan, Gayathri; Thilagar, Sivasudha; Yamini, Rekha; Bera, Soumen; Kathiravan, Arunkumar

doi:10.1016/j.saa.2018.02.030

Cited by 96 publications

(48 citation statements)

References 32 publications

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“…Jhonsi, et al . also found antifungal effects of CDots against C. albicans 61. Similarly, CDots conjugated with ciprofloxacin were tested on Saccharomyces cerevisiae yeast cells, with the observed bright green fluorescence emissions attributed to the CDots inside the cells 90.…”

Section: Anti-fungi Effectsmentioning

confidence: 85%

“…As depicted in Figure 6, the mechanism of action includes the adhesion of CDots to the bacterial surface, the photoinduced production of ROS, the disruption and penetration of the bacterial cell wall/membrane, the induction of oxidative stress with damages to DNA/RNA, leading to the alterations or inhibitions of important gene expressions, and the induction of oxidative damages to proteins and other intracellular biomolecules 12, 58, 59. Under visible/natural light illumination, CDots in contact with the bacteria cell can efficiently generate ROS by activating the oxygen in air or water, leading to the production of hydroxyl free radicals (OH • ) and/or singlet oxygen ( 1 O 2 ), which can destroy some of the critical biomolecules in cell and lead to cell death 60, 61. It is well-documented that ROS induces intracellular protein inactivation, lipid peroxidation, dysfunction of the mitochondria, and gradual disintegration of the cell membrane, followed by necrosis/apoptosis and eventual cell death.…”

Section: Killing/inhibition Of Bacteriamentioning

confidence: 99%

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Carbon Dots as Potent Antimicrobial Agents

et al. 2020

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Carbon dots (CDots) have emerged to represent a highly promising new platform for visible/natural light-activated microbicidal agents. In this article, the syntheses, structures, and properties of CDots are highlighted, representative studies on their activities against bacteria, fungi, and viruses reviewed, and the related mechanistic insights discussed. Also highlighted and discussed are the excellent opportunities for potentially extremely broad applications of this new platform, including theranostics uses.

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Section: Anti-fungi Effectsmentioning

confidence: 85%

Section: Killing/inhibition Of Bacteriamentioning

confidence: 99%

Carbon Dots as Potent Antimicrobial Agents

et al. 2020

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“…These effects are discussed in a review by Anand et al [ 31 ] and have been mentioned by others [ 61 , 62 , 63 , 64 , 65 , 66 ]; they include cell well disruption [ 61 , 62 , 63 , 64 ] and DNA/RNA damage [ 61 , 65 , 66 ] to name a few. Nonetheless, studies demonstrate significant bactericidal capabilities from photosensitization using carbon dots; strains studied by the reports summarized herein are compiled in Figure 4 d, including multi-drug resistant (MDR) E. coli for one study and methicillin-resistant Staphylococcus aureus (MRSA) as well [ 19 ].…”

Section: Carbon Nanodots As Photodynamic Antimicrobial Agentsmentioning

confidence: 99%

Carbon Nanodots in Photodynamic Antimicrobial Therapy: A Review

Knoblauch

Geddes

2020

Materials

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Antibiotic resistance development in bacteria is an ever-increasing global health concern as new resistant strains and/or resistance mechanisms emerge each day, out-pacing the discovery of novel antibiotics. Increasingly, research focuses on alternate techniques, such as antimicrobial photodynamic therapy (APDT) or photocatalytic disinfection, to combat pathogens even before infection occurs. Small molecule “photosensitizers” have been developed to date for this application, using light energy to inflict damage and death on nearby pathogens via the generation of reactive oxygen species (ROS). These molecular agents are frequently limited in widespread application by synthetic expense and complexity. Carbon dots, or fluorescent, quasi-spherical nanoparticle structures, provide an inexpensive and “green” solution for a new class of APDT photosensitizers. To date, reviews have examined the overall antimicrobial properties of carbon dot structures. Herein we provide a focused review on the recent progress for carbon nanodots in photodynamic disinfection, highlighting select studies of carbon dots as intrinsic photosensitizers, structural tuning strategies for optimization, and their use in hybrid disinfection systems and materials. Limitations and challenges are also discussed, and contemporary experimental strategies presented. This review provides a focused foundation for which APDT using carbon dots may be expanded in future research, ultimately on a global scale.

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“…Hypochromism was occur due to π*‐π transition between the base pairs of DNA and compounds. Confirmation of DNA changes and red shift was observed which was due to decrease in energy level of π*‐orbital of compound . However, the secondary structure of DNA was breakage because of the electrostatic interaction or groove binding which leads to the hyperchromic effect along with blue shift.…”

Section: Chemistrymentioning

confidence: 83%

Synthesis, In Vitro Biological Evaluation and In Silico Studies of Some New Heterocyclic Schiff Bases

et al. 2018

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2‐Hydroxy‐naphthaldehyde based heterocyclic Schiff base derivatives (1 a‐1 h) were prepared and characterized by multi‐spectroscopic techniques and elemental analysis. Antibacterial activity of all the compounds was tested against Streptococcus pneumoniae, Enterococcus faecalis, Pseudomonas aeruginosa, Salmonella enterica, Klebsiella pneumoniae and Escherichia coli bacterial strains. The antifungal potential of the synthesized compounds was also tested against three Candida strains (Candida albicans, Candida glabrata and Candida tropicalis). In the antibacterial activity, the compounds showed high MIC values and thus found less potent against all the tested bacterial strains. Interestingly, compounds 1 b and 1 c exhibited significant activity with MIC 125 μg/ml against all the tested fungal strains. Hemolytic assay against human RBCs revealed that compounds 1 b and 1 c showed less toxicity than the standard drug fluconazole at each tested concentration (25‐1000 μg/ml). In growth kinetics studies, compounds 1 b and 1 c significantly inhibited the growth of Candida cells at 2MIC and MIC concentrations. The interaction ability of lead compounds (1 b and 1 c) with Ct–DNA was carried out by absorption, fluorescence, hydrodynamic, cyclic voltammetery measurements and circular dichroism. Results suggested that compound 1 b and 1 c bind to Ct–DNA via an intercalative mode supported by molecular docking studies. The antioxidant potential of heterocyclic derivatives 1 b and 1 c was estimated by DPPH free radical and hydrogen peroxide assay which confirm that compounds exhibited significant antioxidant activity.

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Antimicrobial activity, cytotoxicity and DNA binding studies of carbon dots

Cited by 96 publications

References 32 publications

Carbon Dots as Potent Antimicrobial Agents

Carbon Dots as Potent Antimicrobial Agents

Carbon Nanodots in Photodynamic Antimicrobial Therapy: A Review

Synthesis, In Vitro Biological Evaluation and In Silico Studies of Some New Heterocyclic Schiff Bases

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