Antimicrobial Activity of AgNPs Synthesized Via Green Approach by Using Flowers of Bistortamacrophylla herb of Tungnath Himalaya Region

Abstract

Green synthesis of nano structured materials isan emerging field for the researchers working in the field of chemical, physical, life sciences, engineering and medical sciences, for the welfare of society.In the present study green synthesis of silver nanoparticles (AgNPs)was performed by using flowers of Bistorta macrophylla, a medicinal herb of polygonaceae family of Garhwal Himalayas. For green synthesis of AgNPs, the aqueous flowers extract and the aqueous solution of AgNO3(of 5 milli molar concentration) were well mixed in ratios of 1:9.The metal ion present in the solution reduced Ag+1 to Ag0 within three to four days, which was characterized by color change observation, UV, SEM-EDX, XRD, TEM etc. The absorption maxima of AgNPs of Bistorta macrophylla flowers (BMF) was obtained in the range of 451– 454nm for 5mM concentration of AgNO3, and the SEM images revealed that the average clustersize of synthesized Ag nanoparticles from 5BMF1:9ranges from 20 μm– 23μm, while the EDX analysis four elements show their presence as Ag (51.44 weight %), C (22.45 weight %), Cl (1.18 weight %) and O (22.24 weight%) in the synthesized AgNPs of 5mM(BMF1:9).XRD revealed that the crystals nature of synthesized AgNPs were cubic, and TEM results shows that the biosynthesized nanoparticles are agglomerated and irregular shaped in which size ranging from 12 nm to 38 nm. Synthesized AgNPs were also found to be active for potent antibacterial activity against various pathogenic and harmfull bacterial strains of humans like Pseudomonas Sp.(19.5mm), Bacillus cereus(21 mm) and CoNS (Coagulase Negative Staphylococci) (16.4 mm).Synthesized nanomaterial may be active for efficient clinical applications.

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“…To confirmation of AgNPs formation, EDAX was performed, in which a strong peak of elemental Ag (69.14 weight %) and other peaks of O (22.24 weight %) and C (8.62 weight %) elements were present. The synthesized powdered sample of nano material additionally used for TEM analysis in which it was observed that the green AgNPs were polydispersed, smooth morphology and irregular shaped grains having average size of 43.75nm [12,[18][19][31][32][33][34][35][36] . BET isotherm plot (see Figure .2) of synthesized sample revealed that the synthesized AgNPs have single point surface area (SPSA at P/Po) was 0.1398 m 2 /gm, BET surface area was 0.1057 m 2 /gm, Langmuir surface area (LSA) was 0.1465m 2 /gm, and the micro pore area was 0.7834 m 2 /gm [28] , on the basis of observed data due to which it may become a corrosion free material and may use as a good anti-corrosion material as well as protecting material.…”

Green synthesis of silver nano-particles (AgNPs) from flowers of Rhododendron campanulatum and its potential applications as photo-catalyst and antioxidant

“…To confirmation of AgNPs formation, EDAX was performed, in which a strong peak of elemental Ag (69.14 weight %) and other peaks of O (22.24 weight %) and C (8.62 weight %) elements were present. The synthesized powdered sample of nano material additionally used for TEM analysis in which it was observed that the green AgNPs were polydispersed, smooth morphology and irregular shaped grains having average size of 43.75nm [12,[18][19][31][32][33][34][35][36] . BET isotherm plot (see Figure .2) of synthesized sample revealed that the synthesized AgNPs have single point surface area (SPSA at P/Po) was 0.1398 m 2 /gm, BET surface area was 0.1057 m 2 /gm, Langmuir surface area (LSA) was 0.1465m 2 /gm, and the micro pore area was 0.7834 m 2 /gm [28] , on the basis of observed data due to which it may become a corrosion free material and may use as a good anti-corrosion material as well as protecting material.…”

Mass spectrometry and Nuclear magnetic resonance spectroscopy profiles of red and pink Rhododendron flower petals establish them as rich sources of bioactive secondary metabolites

“…The bark of this species is used to treat jaundice, piles, and liver disorders [8]). The roots of this species are used for the alleviation of various diseases such as cough, headache, boils, and fever [12]. The leaves and flow-ers of R. campanulatum are considered to be toxic but traditionally they are used as a remedy for various ailments.…”