Antimicrobial activity of Apis mellifera L. and Trigona sp. propolis from Nepal and its phytochemical analysis

Okińczyc, Piotr; Paluch, Emil; Franiczek, Roman; Widelski, Jarosław; Wojtanowski, Krzysztof Kamil; Mroczek, Tomasz; Krzyżanowska, Barbara; Skalicka-Woźniak, Krystyna; Sroka, Zbigniew

doi:10.1016/j.biopha.2020.110435

Cited by 38 publications

(40 citation statements)

References 46 publications

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“…Other authors such as Okińczyc et al studied the effect of a Nepalese propolis on C. albicans filamentation and oxidative stress [ 55 ]. Propolis inhibited filamentation, causing a reduction in virulence because the cells became less hydrophobic.…”

Section: Discussionmentioning

confidence: 99%

Antifungal and anti-biofilm activity of a new Spanish extract of propolis against Candida glabrata

Fernández-Calderón

Hernández-González

Gómez-Navia

et al. 2021

BMC Complement Med Ther

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Background Resistance to traditional antifungal agents is a considerable health problem nowadays, aggravated by infectious processes related to biofilm formation, usually on implantable devices. Therefore, it is necessary to identify new antimicrobial molecules, such as natural products, to develop new therapeutic strategies to prevent and eradicate these infections. One promising product is propolis, a natural resin produced by honeybees with substances from various botanical sources, beeswax and salivary enzymes. The aim of this work was to study the effect of a new Spanish ethanolic extract of propolis (SEEP) on growth, cell surface hydrophobicity, adherence and biofilm formation of Candida glabrata, a yeast capable of achieving high levels of resistance to available anti-fungal agents. Methods The antifungal activity of SEEP was evaluated in the planktonic cells of 12 clinical isolates of C. glabrata. The minimum inhibitory concentration (MIC) of propolis was determined by quantifying visible growth inhibition by serial plate dilutions. The minimum fungicide concentration (MFC) was evaluated as the lowest concentration of propolis that produced a 95% decrease in cfu/mL, and is presented as MFC50 and MFC90, which corresponds to the minimum concentrations at which 50 and 90% of the C. glabrata isolates were inhibited, respectively. Influence on cell surface hydrophobicity (CSH) was determined by the method of microbial adhesion to hydrocarbons (MATH). The propolis effect on adhesion and biofilm formation was determined in microtiter plates by measurement of optical density (OD) and metabolic activity (XTT-assay) in the presence of sub-MIC concentrations of SEEP. Results SEEP had antifungal capacity against C. glabrata isolates, with a MIC50 of 0.2% (v/v) and an MFC50 of 0.4%, even in azole-resistant strains. SEEP did not have a clear effect on surface hydrophobicity and adhesion, but an inhibitory effect on biofilm formation was observed at subinhibitory concentrations (0.1 and 0.05%) with a significant decrease in biofilm metabolism. Conclusions The novel Spanish ethanolic extract of propolis shows antifungal activity against C. glabrata, and decreases biofilm formation. These results suggest its possible use in the control of fungal infections associated with biofilms.

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Section: Discussionmentioning

confidence: 99%

Antifungal and anti-biofilm activity of a new Spanish extract of propolis against Candida glabrata

Fernández-Calderón

Hernández-González

Gómez-Navia

et al. 2021

BMC Complement Med Ther

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“…These admixtures are removed during technological processing, and the ethanolic extract of propolis (EEP), obtained as a result of extraction, is a rich set of biologically active substances, including flavonoids, phenolic acids and their esters, lipid-wax substances, bioelements and others [ 1 ]. Therefore, propolis shows antibacterial, antioxidant, anti-inflammatory, antidiabetic, antiallergic, immunomodulatory and anticancer properties [ 10 , 11 , 12 ]. The anticancer potential ingredients have been identified as caffeic acid phenethyl ester, chrysin, artepillin C, galangin, etc.…”

Section: Introductionmentioning

confidence: 99%

Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products

et al. 2021

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Bee products have been known for centuries for their versatile healing properties. In recent decades they have become the subject of documented scientific research. This review aims to present and compare the impact of bee products and their components as antimicrobial agents. Honey, propolis, royal jelly and bee venom are bee products that have antibacterial properties. Sensitivity of bacteria to these products varies considerably between products and varieties of the same product depending on their origin. According to the type of bee product, different degrees of activity were observed against Gram-positive and Gram-negative bacteria, yeasts, molds and dermatophytes, as well as biofilm-forming microorganisms. Pseudomonas aeruginosa turned out to be the most resistant to bee products. An analysis of average minimum inhibitory concentration values for bee products showed that bee venom has the strongest bacterial effectiveness, while royal jelly showed the weakest antibacterial activity. The most challenging problems associated with using bee products for medical purposes are dosage and safety. The complexity and variability in composition of these products raise the need for their standardization before safe and predictable clinical uses can be achieved.

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“…The antiviral activity of propolis can be attributed to the cell lysis and disruption of the cytoplasmic membrane of virus leading to leakage of cellular components and eventually cell death (Górniak et al, 2019, Tagousop et al, 2018. Studies demonstrated that propolis has different antibacterial mechanisms, including inhibition of cell division, collapsing microbial cytoplasm cell membranes and cell walls, inhibition of bacterial motility, different bacterial enzymes activity, what can be cause the weakens the stability of cytoplasmatic membrane (Okińczyca et al, 2020) and acting in bacteriolysis and protein synthesis inhibition (AlAni et al, 2018, Aboody and Mickymaray, 2020, Górniak et al, 2019, Tagousop et al, 2018. Although some studies demonstrated antifungical activity of propolis against Candida genus, this propolis sample did not exhibited antifungical activity this fungus.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

“…Propolis was approved as an official drug in the London pharmacopoeia in the 17th century, due to its antibacterial properties (Przybyłek and Karpiński, 2019;Okińczyca et al, 2020, Hochheim et al, 2019. The antimicrobial activity of propolis and geopropolis was demonstrated in clinical, in vivo, and in vitro studies (Cornara et al, 2017, Pasupuleti et al, 2017, Siheri et al, 2016.…”

Section: Antimicrobial Activitymentioning

confidence: 99%

Antimicrobial activity of flavonoids glycosides and pyrrolizidine alkaloids from propolis of Scaptotrigona aff. postica

Campos

Silva

Negri

et al. 2021

Preprint

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Stingless bees belonging to the Meliponinae subfamily, are known as meliponines. Scaptotrigona affinis postica Latreille, 1807 from northeast of Brazil is popularly known as tubi in Maranhao State. Scaptotrigona, which is widely distributed in neotropical regions, includes species that build their hives in pre-existing cavities. Flavones di-C-glycosides, and the pyrrolizidine alkaloid 7-(3-methoxy-2-methylbutyryl)-9-echimidinylretronecine were reported previously in propolis from S. postica. Fractions 40 AEP and 40 MEP from ethanolic extract were analyzed by LC-MS. The chromatographic profile of fractions 40 AEP and 40 MEP revealed the presence of many pyrrolizidine alkaloids, among them, lithosenine (14), lithosenine arabinoside (19), 7-angeloyl-9-(2,3-dihydroxybutyryl) retronecine (1), 7-(2-methylbutyryl) retronecine (3), 9-sarracinoylretronecine (13) and viridinatine (8), besides the flavonoids schaftoside (15), aromadendrin-7-O-methyl ether (12), 7-methoxy-5,6,3,4,5,6-hexahydroxy-flavone-3-O-glucuronide (11), mangiferin (10) and 3-O-methyl mangiferin (17). Fractions 40 AEP and 40 MEP showed antimicrobial activity against Gram positive bacteria, including Escherichia coli D31- streptomycin resistant. Cell viability was expressed in terms of the relative absorbance of treated and untreated cells (control). There was no statistical difference between treated and untreated cells.

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Antimicrobial activity of Apis mellifera L. and Trigona sp. propolis from Nepal and its phytochemical analysis

Cited by 38 publications

References 46 publications

Antifungal and anti-biofilm activity of a new Spanish extract of propolis against Candida glabrata

Antifungal and anti-biofilm activity of a new Spanish extract of propolis against Candida glabrata

Promising Antimicrobial Properties of Bioactive Compounds from Different Honeybee Products

Antimicrobial activity of flavonoids glycosides and pyrrolizidine alkaloids from propolis of Scaptotrigona aff. postica

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