Antimicrobial Activity of Different Types of Mouthwashes Against Streptococcus Mutans , Staphylococcus Aureus and Candida Albicans : In Vitro Study

Aldhaher, Zainab A.

doi:10.12816/0014954

Cited by 6 publications

(4 citation statements)

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“…Previous research has found that patients with blood problems had a greater S. mutans and Lactobacillus count in their saliva than healthy people. 27,28 The presence of a certain microorganism was not revealed by an increase…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Oral Hygiene Status, Salivary Fluoride Concentration and Microbial Level in Thalassemic and Hemophilic Patients

et al. 2022

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Objective: This study aimed to evaluate oral hygiene status, salivary fluoride concentration, and Streptococcus mutans and Lactobacillus levels in saliva of thalassemic, hemophilic and individuals without any other systemic disorders.Materials and Methods: A total 162 individuals (44 healthy individuals, 86 thalassemic and 32 hemophilic patients) were selected, and randomly (n=30 in each group), the patients were allocated to Group A: individuals without any systemic condition, Group B: thalassemic patients, and Group C: hemophilic patients. Detailed case history, DMFT/DMFS, and OHI-S index were recorded. An aliquot of 5 ml of saliva was collected from each patient to determine the salivary fluoride concentration and predominant microbial colony in saliva. The data were analyzed by chi-square test of independence and nonparametric Kruskal-Wallis H test.Results: The mean debris and calculus index among groups A, B, and C was 0.55 ± 0.43, 0.61 ± 0.46, 0.46 ± 0.47 and 0.33 ± 0.48, 0.18 ± 0.34, and 0.15 ± 0.34, respectively. The DMFT score for group A was high (1.93 ± 1.86, 1.67 ± 1.92) compared to groups B (0.40 ± 0.77, 0.67 ± 1.37) and C (0.47 ± 0.68, 0.30 ± 0.54). The fluoride concentrations among three groups (A, B, and C) were 0.06 ± 0.07, 0.12 ± 0.13, and 0.12 ± 0.13 ppm respectively. The number of colony-forming units was highest in the healthy individual>hemophilic>thalassemic and presence of predominant microorganisms showed insignificant association among the groups (p=0.323).Conclusion: Compared to healthy individuals, thalassemic and hemophilic patients had better oral hygiene.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Oral Hygiene Status, Salivary Fluoride Concentration and Microbial Level in Thalassemic and Hemophilic Patients

et al. 2022

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“…Gram staining was performed by applying a primary stain (crystal violet) to a heat-fixed smear for one minute, followed by the addition of a mordant (Gram's Iodine) for one-minute, rapid decolorization with alcohol, acetone, or a mixture of alcohol and acetone for 30 seconds and lastly, counterstaining with safranin for about one minute. The slides were washed thoroughly with distilled water, air dried and observed under the microscope (Aldhaher ZA, 2014).…”

Section: Gram Stainingmentioning

confidence: 99%

“…This study includes the preparation of anti-bacterial herbal mouthwash using the aqueous extracts of leaves from four plants namely Ocimum tenuiflorum (Tulsi), Plectranthus amboinicus (Mexican mint), Mentha (Mint), Foeniculum vulgare (Fennel) that acts against the oral pathogens Staphylococcus aureus, Streptococcus pyogenes, Bacillus sp. Further the Anti-microbial activity of the leaf extracts was analysed using Agar well diffusion method (Aldhaher ZA. 2014).…”

Section: Introductionmentioning

confidence: 99%

Effect of Herbal Extracts on Oral Microorganisms

Pierry

2022

kjcab

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The mouth has a diverse microbial ecosystem and is yet vulnerable to infectious diseases. Dental plaque is formed when bacteria accumulate on teeth, and if left untreated, it can develop to gingivitis. Gingivitis can progress to periodontitis, which causes irreparable damage to the gums and underlying support tissues if left untreated. Hundreds of bacterial species are involved in dental caries such as streptococci species, lactobacillus species. Mouthwashes are meant to reduce oral bacteria, remove any food debris and provide a pleasant and refreshing taste in the mouth. Antiseptic chemicals including alcohol, menthol, and eucalyptol are used in mouthwash to destroy microorganisms. Plaque and gingivitis can be avoided with the use of mouthwash. Each mouthwash mixture has somewhat different chemicals, and each product serves a particular purpose. Herbal mouthwashes are considered to be an effective alternative for the commercial products. Herbal mouthwashes are in high demand, because they have less or no side effects and act on oral pathogens effectively. Herbs, in contrast to artificial products, are widely regarded as highly effective. Medicinal herbs have long been used to treat ailments because of their antibacterial and antifungal properties against human pathogens. Herbal mouth washes have the ability to deliver the therapeutic ingredients to access against the organism present on the surface of the mouth. Dental caries and periodontal diseases are among the most frequent infectious diseases experienced by many people at various phases of their lives, with a significant prevalence among children and adolescents who do not practice basic oral hygiene. Herbal mouthwash was prepared from the aqueous extracts of four different leaves namely Ocimum tenuiflorum, Plectranthus amboinicus, Mentha and Foeniculum vulgare. The antimicrobial efficacy was tested against the oral pathogens Staphylococcus sp., Streptococcus sp., and Bacillus sp. by using agar well diffusion method. The herbal mouthwash was found to be effective against the oral pathogens.

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“…It is considered a part of the commensal human microflora. However, Candida albicans may quickly become pathogenic if the host's immune system deteriorates or the environment changes (11) (34) . It commonly results in nosocomial infections and can infiltrate superficial and deep organs in humans (12) .…”

Section: Introductionmentioning

confidence: 99%

Investigating the impact of non-nutritive sweeteners on the antifungal potential of alcoholic and aqueous Eucalyptus extracts against salivary candida albicans (An in-vitro study)

AL-Qaralusi,

Al-Mizraqchi

2023

J Bagh Coll Dent

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Background: Eucalyptus extracts and derivatives are natural substances with potent antimicrobial properties. This study investigated the in- vitro effects of non-nutritive sweeteners on the antifungal activity of alcoholic and aqueous Eucalyptus extracts against Candida albicans, a common oral pathogen. Materials and Method: Ten isolates of Candida albicans were isolated from dental students’ salivary samples. The alcoholic and aqueous extracts were prepared from fresh Eucalyptus leaves using maceration. The sensitivity of Candida albicans isolates to various concentrations of Eucalyptus extracts ranging from 50 to 250 (mg/mL) was evaluated via agar well diffusion method, while the agar streaking method was used to assess the minimum fungicidal concentration (MFC). In addition, the effect of non-nutritive sweeteners on the MFC of the extracts was investigated. Results: The Eucalyptus extract-sensitive Candida albicans isolates showed an increase in inhibitory zone width with increasing extract concentration. Regarding their antifungal effectiveness, clear disparities were observed among extract concentrations. Against Candida albicans, the MFC for Eucalyptus alcoholic extract was 75 mg/mL, but the MFC for Eucalyptus aqueous extract was 200 mg/mL. Notably, 15% stevia and 5% sucralose did not affect the antifungal effects of the Eucalyptus alcoholic extract. The antifungal effectiveness of the aqueous Eucalyptus extract against Candida albicans was unaffected by stevia and sucralose concentrations of up to 1%. Conclusion: Significant antimicrobial action against Candida albicans is shown in Eucalyptus extracts. Results indicated that stevia and sucralose at specific quantities could be utilized as sweeteners for Eucalyptus extracts in an efficient manner without impairing the extracts’ antifungal activity.

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Antimicrobial Activity of Different Types of Mouthwashes Against Streptococcus Mutans , Staphylococcus Aureus and Candida Albicans : In Vitro Study

Cited by 6 publications

References 0 publications

Evaluation of Oral Hygiene Status, Salivary Fluoride Concentration and Microbial Level in Thalassemic and Hemophilic Patients

Evaluation of Oral Hygiene Status, Salivary Fluoride Concentration and Microbial Level in Thalassemic and Hemophilic Patients

Effect of Herbal Extracts on Oral Microorganisms

Investigating the impact of non-nutritive sweeteners on the antifungal potential of alcoholic and aqueous Eucalyptus extracts against salivary candida albicans (An in-vitro study)

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