Antimicrobial Activity of Echinophora tenuifolia L. and Raphanus sativus L. Extracts

Törün, Bahadır; Çoban, Esin Poyrazoğlu; H, Halil Biyik; Barişik, Esra

doi:10.5530/ijper.51.3s.50

Cited by 3 publications

(1 citation statement)

References 2 publications

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“…Then, the extract was filtered and concentrated by rotary evaporator. The dry powder extracts (0.5-1.0g) were kept at +4 o C and the extracts were dissolved in 5% DMSO just before the activity studies were started (Törün, Çoban, Bıyık, & Barışık, 2017;Çoban, Bıyık, Törün, &Yaman, 2017).…”

Section: Preparation Of Plant Extractsmentioning

confidence: 99%

Investigation of the antimicrobial activities of solvent extracts of two endemic species from Turkey: Campanula tomentosa Lam. and Verbascum mykales Bornm.

Çoban¹,

Barişik²

2021

ijp

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Background and Aims: Campanula tomentosa Lam. and Verbascum mykales Bornm. are endemic species in Turkey. Extracts of these plants contain important natural compounds such as flavonoids, saponins and tannins. This study investigates the antimicrobial effects of leaf extracts of C. tomentosa and V. mykales against some bacteria and yeasts. Materials and Methods:Leaves of plant samples were air-dried and ground into powder. Five solvents (ethyl acetate, methanol, acetone, chloroform, boiled water) were used for extraction. Experiments were conducted using these crude extracts on seventeen bacteria, three yeasts and three microfungi. The agar well diffusion method was used for the antimicrobial activities of the extracts. In addition, minimum inhibitory concentrations, minimum bacteriocidal concentration, minimal fungicidal concentrations were carried out. Results:The ethyl acetate and methanol extracts of C. tomentosa and V. mykales were found to be highly effective against the tested microorganisms. According to the MIC values, the ethyl acetate extracts of C. tomentosa and V. mykales had a strong effect (4-8 μg/mL) against Escherichia coli ATCC 35218, Micrococcus luteus ATCC 9341, Streptococcus pneumonia ATCC 27336, Pseudomonas aeruginosa ATCC 35032, Mycobacterium smegmatis ATCC 607, Proteus vulgaris ATCC 33420, Enterococcus faecalis ATCC 29212, Bacillus cereus ATCC 11778, and Bacillus subtilis ATCC 6633. The ethyl acetate extract of C. tomentosa had a moderate effect (64 μg/mL) against Candida albicans ATCC 10231 and Saccharomyces cerevisiae ATCC 9763, and the ethyl acetate extract of V. mykales had a moderate effect (64 μg/mL) against Aspergillus flavus ATCC 9807 and Aspergillus niger ATCC 16404. However, the boiled water extract of C. tomentosa and V. mykales had no effect on the tested microorganisms. Conclusion:C. tomentosa and V. mykales used in the study are endemic plants and their antimicrobial activities are being investigated for the first time. The ethyl acetate extract of both plants was found to be most effective against the Gram (+) and Gram (-) bacteria. However, all extracts of both plants were found to have fewer antimicrobial effects against used yeasts and microfungi. This study demonstrates that plant extracts are more effective against prokaryotic microorganisms than eukaryotes.

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Section: Preparation Of Plant Extractsmentioning

confidence: 99%

Investigation of the antimicrobial activities of solvent extracts of two endemic species from Turkey: Campanula tomentosa Lam. and Verbascum mykales Bornm.

Çoban¹,

Barişik²

2021

ijp

View full text Add to dashboard Cite

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Bioactivity of Raphanus Species against Agricultural Phytopathogens and its Role in Soil Remediation: A Review

Seimandi

Imhoff

Derita

2024

CCHTS

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Phytopathogens and weeds represent around 20-40% of global agricultural productivity losses. Synthetic pesticide products are the most used to combat these pests, but it reiterates that their use has caused tremendous pressure on ecosystems' self-cleansing capacity and resistance development by pathogens to synthetic fungicides. In the last decades, researchers have demonstrated the vast biological properties of plants against pathogens and diseases. Raphanus species (Brassicaceae) possesses antimicrobial, antioxidant, anti-inflammatory, anticancer, hepatoprotective, antidiabetic, insecticidal, nematicidal, allelopathic, and phytoremediators properties. These are due to the presence of structurally diverse bioactive compounds, such as flavonoids and glucosinolates. In this review, we have provided an update on the biological properties of two Raphanus species (R. sativus and R. raphanistrum), detailing the type of natural product (extract or isolated compound), the bioassays displayed, and the results obtained for the main bioactivities of this genus cited in the literature during the last 30 years. Moreover, preliminary studies on phytopathogenic activities performed in our laboratory have also been depicted. We conclude that Raphanus species could be a source of natural bioactive molecules to treat phytopathogens and weeds that affect crops and remediate contaminated soils.

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Nematicidal effect of powder extractions of different coloured radish seeds against Meloidogyne incognita on tomato

Göze Özdemir,

Maril,

Çimenkaya

et al. 2024

International Journal of Agriculture Environment and Food Sciences

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The study aimed to evaluate the nematicidal effect of powder extracts of different coloured radish seeds against Meloidogyne incognita on tomato (Gülizar F1, susceptibile to root-knot nematode) under controlled conditions. Extractions were obtained from radish seeds of different colours (white, black, red, yellow) by using ethanol and acetone solvents. The experiment was carried out using 2, 4 and 6 g powder/plant application of the extracts in the pot. The experiment was set up in a random plot design with 5 replication for each radish seed extract and concentration. Radish seed powder was applied one week after nematode inoculation (1000 M. incognita eggs). After 50 days, the number of galls and egg masses on the roots were counted. It was determined that radish colour, extraction solvents and concentrations of extracts differed significantly for their nematicidal effects. The mean number of galls and egg masses was found to be 56 units in the negative control. Compared to the negative control, all treatments and concentrations decreased the number of galls and egg masses. The number of galls and egg masses was lower in acetone extract than in ethanole extract. The nematicidal effect was higher in yellow and red radish seeds powder application. The highest nematicidal effect was determined at 6 g powder/plant application. While the mean number of galls was 1.4 unit in the yellow seed powder application at a concentration of 6 g/plant of the extract prepared with acetone solvent, it was found to be 3.0 units in the red seed powder application at 6 g/plant of the acetone extract. The number of egg masses was 1.0 unit in the yellow seed powder application, while it was 2.8 units in the red seed powder application at 6 g/plant of the acetone extract. The acetone extract of radish seed powder can be used as an alternative to chemicals in the root-knot nematodes control.

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Antimicrobial Activity of Echinophora tenuifolia L. and Raphanus sativus L. Extracts

Cited by 3 publications

References 2 publications

Investigation of the antimicrobial activities of solvent extracts of two endemic species from Turkey: Campanula tomentosa Lam. and Verbascum mykales Bornm.

Investigation of the antimicrobial activities of solvent extracts of two endemic species from Turkey: Campanula tomentosa Lam. and Verbascum mykales Bornm.

Bioactivity of Raphanus Species against Agricultural Phytopathogens and its Role in Soil Remediation: A Review

Nematicidal effect of powder extractions of different coloured radish seeds against Meloidogyne incognita on tomato

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