Antimicrobial Activity ofPimpinella anisumandFoeniculum vulgareEssential Oils AgainstPaenibacillus larvae

Gende, Liesel Brenda; Maggi, Matías; Fritz, R.; Eguaras, Martín Javier; Bailac, Pedro N.; Ponzi, Marta I.

doi:10.1080/10412905.2009.9700120

Cited by 57 publications

(41 citation statements)

References 9 publications

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“…Some essential oils have a considerable potential for controlling American Foulbrood, disease caused by a sporulated bacteria Paenibacillus larvae (Gende et al 2009), chalkbrood disease originated by Ascosphaera apis (Dellacasa et al 2003) and the parasitic mites, Acarapis woodi and V. destructor (Calderone et al 1997).…”

Section: Discussionmentioning

confidence: 99%

Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae)

et al. 2009

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Varroa destructor is an external parasitic mite that is a serious pest of honeybees and has caused severe losses of colonies worldwide. One of the feasible alternative treatments being used for their control is essential oils. The aim of this work was to evaluate the bioactivity of some essential oils on V. destructor and Apis mellifera in relation with their chemical composition and physicochemical properties. Lavender, lavendin and laurel essential oils showed linalool as main compound in their composition. 1,8-Cineole was also present as a predominant component in the laurel essential oil. However, thyme oil was characterized by a high concentration of thymol. Mites and bees toxicity was tested by means of complete exposure method. For mites, LC(50) values for laurel, lavender and lavendin essential oil did not show significant variation throughout all observation times. However, the LC(50) values for thyme oil at 48 and 72 h were lower than at 24 h. Bee mortality was evident only in treatment with thyme oil. At 48 and 72 h, lavender essential oil presented better selectivity indexes. In this research, all essential oils caused mite mortality without severe harmful effects on adult bees. The simultaneous evaluation of the physicochemical analysis of the essential oils, the characterization of the dosage response relationships among them, and the mortality effects on mite and bees, give us the possibility to obtain comparative results for future research in Varroa control.

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Section: Discussionmentioning

confidence: 99%

Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae)

et al. 2009

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“…Tukey's means separation test for MIC and MBC values indicated that the essential oil of citronella grass (C. nardus) and niaouli (M. viridiflora) were different from the other two essential oils tested (α < 0.01) and were no different between them in their response against the bacterial strains (Table 3). There is not literature about the efficacy of citronella grass and niaouli essential oils to control AFB, though there are previous studies about the use of natural products against the causative agent of this disease (Eguaras et al, 2005;Fuselli et al, 2006Fuselli et al, , 2007Fuselli et al, , 2008aFuselli et al, ,b, 2009Gende et al, 2008Gende et al, , 2009. Citronella grass and niaouli essential oils showed good antimicrobial activity, comparable to that reported by Alippi et al (1996) for savory (Satureja hortensis) and oregano (Origanum vulgare) essential oils, with similar MIC values ranging from 250-450 mg L -1 and to Artemisia absinthium, A. annua and Lepechinia floribunda oils with MIC mean values of 416.7 mg L -1 , 401.9 mg L -1 and 393.6 mg L -1 , respectively (Fuselli et al, 2008b).…”

Section: Resultsmentioning

confidence: 99%

In vitro antibacterial effect of exotic plants essential oils on the honeybee pathogen Paenibacillus larvae, causal agent of American foulbrood

Fuselli

Rosa

Eguaras

et al. 2010

Span. j. agric. res.

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Chemical composition and antimicrobial activity of exotic plants essential oils to potentially control Paenibacillus larvae, the causal agent of American foulbrood disease (AFB) were determined. AFB represents one of the main plagues that affect the colonies of honeybees Apis mellifera L. with high negative impact on beekeepers worldwide. Essential oils tested were niaouli (Melaleuca viridiflora) and tea tree (Melaleuca alternifolia) from Myrtaceae, and citronella grass (Cymbopogon nardus) and palmarosa (Cymbopogon martinii) from Gramineae. The components of the essential oils were identified by SPME-GC/MS analysis. The antimicrobial activity of the oils against P. larvae was determined by the broth microdilution method. In vitro assays of M. viridiflora and C. nardus oils showed the inhibition of the bacterial strains at the lowest concentrations tested, with minimal inhibitory concentration (MIC) mean value about 320 mg L -1 for both oils, respectively. This property could be attributed to the kind and percentage of the components of the oils. Terpinen-4-ol (29.09%), α-pinene (21.63%) and limonene (17.4%) were predominant in M. viridiflora, while limonene (24.74%), citronelal (24.61%) and geraniol (15.79%) were the bulk of C. nardus. The use of these essential oils contributes to the screening of alternative natural compounds to control AFB in the apiaries; toxicological risks and other undesirable effects would be avoided as resistance factors, developed by the indiscriminate use of antibiotics.Additional key words: antimicrobial activity, chemical composition, Cymbopogon martini, Cymbopogon nardus, Melaleuca alternifolia, Melaleuca viridiflora, honeybees' pathogen. ResumenActividad antibacteriana in vitro de los aceites esenciales de plantas exóticas frente al patógeno bacteriano Paenibacillus larvae, agente causal de loque americana en colonias de abejas melíferas Se determinó la composición química y se evaluó la actividad antimicrobiana in vitro de cuatro aceites esenciales de plantas exóticas para el control del patógeno bacteriano Paenibacillus larvae, agente causal de loque americana, que afecta a los estadios de larva y pupa de la abeja melífera (Apis mellifera L.). Esta enfermedad produce grandes pérdidas económicas en la apicultura mundial. Los aceites esenciales analizados, pertenecientes a la familia Myrtaceae y Gramineae, fueron: niaouli (Melaleuca viridiflora) y árbol del té (Melaleuca alternifolia), citronela (Cymbopogon nardus) y palmarosa (Cymbopogon martinii), respectivamente. La composición química de los aceites esenciales se efectuó por cromatografía de gases acoplada a un espectrómetro de masas (CG-EM). La concentración inhibitoria mínima (MIC) se determinó por el método de microdilución en caldo y la concentración bactericida mínima (MBC) en agar MYPGP. Los aceites esenciales de niaoulí y citronela registraron valores promedio de MIC de 320 mg L -1 , atribuidos al tipo y porcentaje de sus componentes: terpinen-4-ol (29,09%), α-pineno (21,63%) y limoneno (17,4%) que predominaron ...

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“…The use natural antimicrobial substances have been suggested as promising alternatives to control AFB (Bastos et al 2008;Gende et al 2009). Flesar et al (2010) tested the antibacterial activity of different natural compounds (flavonoids, alkaloids, terpenoids) and crude extracts of plants against P. larvae.…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial factor from Bacillus amyloliquefaciens inhibits Paenibacillus larvae, the causative agent of American foulbrood

et al. 2011

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Bacillus amyloliquefaciens LBM 5006 produces an antimicrobial factor active against Paenibacillus larvae, a major honeybee pathogen. The antagonistic effect and the mode of action of the antimicrobial factor were investigated. The antibacterial activity was produced starting at mid-logarithmic growth phase, reaching its maximum during the stationary phase. Exposure of cell suspensions of P. larvae to this antimicrobial resulted in loss of cell viability and reduction in optical density associated with cell lysis. Scanning electron microscopy showed damaged cell envelope and loss of protoplasmic material. The antimicrobial factor was stable for up to 80°C, but it was sensitive to proteinase K and trypsin. Mass spectrometry analysis indicates that the antimicrobial activity is associated with iturin-like peptides. The antimicrobial factor from B. amyloliquefaciens LBM 5006 showed a bactericidal effect against P. larvae cells and spores. This is the first report on iturin activity against P. larvae. This antimicrobial presents potential for use in the control of American foulbrood disease.

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Antimicrobial Activity ofPimpinella anisumandFoeniculum vulgareEssential Oils AgainstPaenibacillus larvae

Cited by 57 publications

References 9 publications

Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae)

Acaricidal and insecticidal activity of essential oils on Varroa destructor (Acari: Varroidae) and Apis mellifera (Hymenoptera: Apidae)

In vitro antibacterial effect of exotic plants essential oils on the honeybee pathogen Paenibacillus larvae, causal agent of American foulbrood

Antimicrobial factor from Bacillus amyloliquefaciens inhibits Paenibacillus larvae, the causative agent of American foulbrood

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