Antimicrobial activity of &lt;i&gt; Terminalia catappa&lt;/i&gt;, &lt;i&gt; Manilkara zapota&lt;/i&gt; and &lt;i&gt; Piper betel&lt;/i&gt; leaf extract

Nair, Rathish; Chanda, Sumitra

doi:10.4103/0250-474x.43012

Cited by 86 publications

(20 citation statements)

References 11 publications

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“…The agar well diffusion method was used to test the anti-microbial effect of N. sativa crude extracts [27] . All media plates (9 cm in diameter) were prepared according to the manufacturer recommendations (Difco Laboratories, USA).…”

Section: Agar Well Diffusion Assaymentioning

confidence: 99%

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Abu-Al-Basal¹

2009

American J. of Applied Sciences

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Problem statement:The developing microbial resistance to the existing anti-microbial agents has become a real challenge and a serious problem facing patients suffering from skin infections. Seeds of Nigella sativa have been used for a long time in folk medicine for the treatment of such infections. Production of new potent agents is urgently needed, especially for hospitals and health centers. Therefore, the anti-microbial effect of aqueous, diethyl ether, chloroform and petroleum ether extracts of the seeds against four standard microbial strains and seven clinical isolates from patients with skin wound infections were investigated. Approach: The in vitro anti-microbial effect of the extracts at a concentration of 20% on standard strains and clinical isolates was assessed and compared with standard drugs, chloramphenicol and amphotericin B using agar well diffusion assay. The in vivo anti-bacterial effect of petroleum ether extract was studied in male BALB/c mice infected subcutaneously with S. aureus (ATCC 25923) or a clinical isolate (0.1 mL from 10 9 colony forming units mL −1 suspension) and immediately treated at the infected site by subcutaneous injection of 0.1 mL of pure extract (fixed oil) or chloramphenicol or normal saline. Counts of viable bacteria present in the skin area corresponding to the infected site were determined, after 24 and 48 h of infection and treatment. Results: The aqueous extract did not show any inhibitory effect against all the tested microorganisms. The diethyl ether and chloroform extracts indicated significant inhibitory effect only against Gram-positive bacteria. However, petroleum ether extract was proved to be the most powerful one against these bacteria and also against other clinical isolates like one Gram-negative bacterium (Klebsiella pneumonia) and the yeast (Candida albicans). Moreover, the extract revealed a superior effect over the standard drug, chloramphenicol, on the clearance of subcutaneous staphylococcal infection in mice when injected at the site of infection. Counts of viable bacteria were decreased at highly significant level in mice infected with S. aureus (ATCC 25923) or a clinical isolate. Conclusion/Recommendations:The results of this study revealed clear potentiality of N. sativa fixed oil as a source for anti-microbial drugs and support its use in folk medicine for the treatment of microbial skin infections.

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Section: Agar Well Diffusion Assaymentioning

confidence: 99%

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Abu-Al-Basal¹

2009

American J. of Applied Sciences

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“…plant has been investigated in various pharmaceutical studies as it contains a variety of chemical components (Pandya et al, 2013; Yeh et al, 2014). T. catappa L. leaf extracts exhibit biological activities, including antioxidant (punicalagin, punicalin, terfluvina A and B, chebulic acid, benzoic acid, cumaric, and its derivatives) (Chen and Li, 2006; Chyau et al, 2006; Kinoshita et al, 2007), antidiabetic (β-carotene) (Anand et al, 2015), anticancer (punicalagin) (Naitik et al, 2012), antiviral (ellagic acid) (Tan et al, 1991), anti-inflammatory (triterpenic acids, especially ursolicacid and its derivatives) (Fan et al, 2004), antimicrobial (flavones and flavanols) (Kloucek et al, 2005; Nair and Chanda, 2008; Shinde et al, 2009), and hepato-protective activities (punicalagin, punicalin) (Kinoshita et al, 2007). …”

Section: Introductionmentioning

confidence: 99%

“…In India, a plaster of T. catappa leaves is used to treat scabies, leprosy wounds and other skin diseases (Nair and Chanda, 2008). Its traditional use includes the treatment of diarrhea and fever, especially in India, the Philippines and Malaysia (Kloucek et al, 2005; Shinde et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Characterization of Terminalia catappa Linn. Extracts and Their antifungal Activities against Candida spp.

Terças

Monteiro²,

Moffa³

et al. 2017

Front. Microbiol.

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Terminalia catappa Linn bark is used to treat dysentery by various populations in Southeast Asian countries, and its leaves have also been used in traditional medicine to treat hepatitis in India and the Philippines. Here, the antifungal actions of crude hydro-alcoholic extract (TcHE) and fractions from T. catappa leaves were assessed via the agar diffusion and microdilution tests on Candida reference strains and clinical isolates from patients with acquired immunodeficiency syndrome (AIDS). Additionally, the potential cytotoxic effects of TcHE were assessed on cultured human peripheral blood mononuclear cells (PBMC). T. catappa fractions and sub-fractions were analyzed by gas chromatography coupled to mass spectrometry with electron impact (GC/MS/EI), high-performance liquid chromatography coupled to mass spectrometry “electrospray” ionization in positive mode (HPLC/MS/MS/ESI+) and hydrogen nuclear magnetic resonance (1HNMR). TcHE and its fractions were able to inhibit the growth of all tested Candida strains with the n-butanol (FBuOH) fraction presenting the best antifungal activity. Testing of different FBuOH sub-fractions (SF) showed that SF10 was the most active against Candida spp. Fractioning of SF10 demonstrated that 5 out of its 15 sub-fractions were active against Candida spp., with SF10.5 presenting the highest activity. Chemical analysis of SF10 detected hydrolysable tannins (punicalin, punicalagin), gallic acid and flavonoid C-glycosides. Overall, the results showed that T. catappa L. leaf extract, fractions and sub-fractions were antifungal against Candida spp. and may be useful to treat diseases caused by this fungus.

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“…The methanolic extract is significantly more efficient than the aqueous extract in inhibiting the investigated microbial strains. [6] Shikhamandloi et al [4] found that the methanolic extract shows antifungal activity against Curvularia lunata , Aspergillus niger , Penicillium chrysogenum , and Trichophyton tonsurans .…”

Section: Botanical Descriptionmentioning

confidence: 99%

An updated review of Terminalia catappa

Anand¹,

Divya²,

Kotti³

2015

Phcog Rev

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Terminalia catappa Linn. is known for its nutritional fruit and possesses medicinal benefits as well. This is a comprehensive review of the phytoconstituents and pharmacological benefits. T. catappa has been recognized for its medicinally essential phytoconstituents, such as phenol, flavonoid, and carotenoid. Numerous pharmacological investigations have confirmed this plant's ability to exhibit antimicrobial, anti-inflammatory, antidiabetic, antioxidant, hepatoprotective, and anticancer activities, all of which support its traditional uses.

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Antimicrobial activity of <i> Terminalia catappa</i>, <i> Manilkara zapota</i> and <i> Piper betel</i> leaf extract

Cited by 86 publications

References 11 publications

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

In vitro and In vivo Anti-Microbial Effects of Nigella sativa Linn. Seed Extracts Against Clinical Isolates from Skin Wound Infections

Phytochemical Characterization of Terminalia catappa Linn. Extracts and Their antifungal Activities against Candida spp.

An updated review of Terminalia catappa

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