Antimicrobial activity of natural products againstClostridium difficile in vitro

Abstract

This study encourages further investigation of natural products for treatment of CDI.

Effect of natural products on the production and activity of Clostridium difficile toxins in vitro

“…The MICs of natural products (Table 1 ) 14 and antimicrobial controls for the four strains of C . difficile have previously been published 13 and are shown in Table S1 . The only exception was the two extra Leptospermum honeys A and B as listed in Table 1 .…”

“…Processed and pure products (n = 12) were purchased from pharmacies and health food stores in Perth, WA, or from the manufacturers (Table 1 ). The products were prepared for testing as previously described 13 . Fidaxomicin and vancomycin were used as antimicrobial controls in susceptibility testing and fidaxomicin was used as an effective-antimicrobial control in tissue culture and ELISA assays.…”

“…Minimum inhibitory concentrations (MIC) for natural products and comparator antimicrobial agents were determined by the broth microdilution method as previously described 13 . Doubling dilutions of 20% DMSO were prepared and tested in parallel (solvent control), and fidaxomicin and vancomycin were included as antibiotic controls.…”

“…We have shown recently that several natural products have antimicrobial activity against C . difficile in vitro 13 . Given that CDI is a toxin-mediated disease, the purpose of the present study was to investigate the effect of those previously tested natural products, and corresponding pure substances, on toxin production and activity in C .…”

Irritable bowel syndrome: modern concepts and new possibilities of correction

“…К другим позитивным эффектам МПМ относят антибактериальное ее действие в отношении Clostridium difficile и Candida [20,22] . Под действием МПМ нарушается чувства кворума у многих микроорганизмов, что приводит к разрушению биопленки и снижению патогенности Pseudomonas aeruginosa и Aeromonas hydrophila [16] .…”