Antimicrobial activity of nudibranch Chromodoris lineolata associated bacteria against skin diseases pathogens from Jepara Coastal Waters, Indonesia

Abstract

Abstract. Wijaya AP, Sabdono A, Sibero MT, Trianto A, Radjasa OK. 2022. Antimicrobial activity of nudibranch Chromodoris lineolata associated bacteria against skin diseases pathogens from Jepara Coastal Waters, Indonesia. Biodiversitas 23: 1911-1919. The emergence of multidrug-resistant (MDR) skin pathogens has caused the treatment of skin diseases to become increasingly ineffective using by conventional antibiotics. As a source of antimicrobial compounds against MDR pathogens, marine invertebrate-associated bacteria have been widely known for their valuable biological activity. Therefore, this study aims to determine the antimicrobial activity of nudibranch Chromodoris lineolata associated bacteria against various skin pathogens and to detect the presence of biosynthetic gene clusters (BGC) through a molecular approach. A total of 14 nudibranch-associated bacteria were successfully isolated from Awur Bay and Panjang Island. Antimicrobial activity screening using the agar plug method indicated that three bacterial strains showed antimicrobial activity against two skin pathogens, Propionibacterium acnes and Malassezia furfur. Based on the biochemical test, these potential strains were classified into the Terrabacteria and Proteobacteria groups. Through 16S rRNA gene sequencing, potential strains were identified as Streptomyces lateritius, Labrenzia marina, and Halomonas meridiana through 16S rRNA gene sequencing. The results of BGC detection showed the presence of a type II polyketide synthase (PKS II) biosynthetic gene cluster in Streptomyces lateritius and Labrenzia marina strains at 600bp-650bp.

Characterization and identification of extracellular polysaccharides-degrading enzymes (epes)-producing marine sediment bacteria

“…Plenty of microorganism have been discovered from Panjang Island ecosystem, from prokaryotic like bacteria to eukaryotic microorganisms such as fungi [17][18][19] . Our previous study has succeeded in obtaining various species of association bacteria from this location such as Salinicoccus roseus, Sphingobium yanoikuyae, Streptomyces lateritius, Labrenzia marina, and Halomonas meridiana [20,21]. However, research on marine sediment bacteria has not been carried out.…”

“…The result of the bacterial characterization is presented in Table 1 and Table 2. Morphological, cells morphology, and biochemical characteristics data were used to classification the group of the bacteria [21]. According to gram staining, eight strain were identified as gram-positive bacteria and three other strain were identified as gram-negative bacteria.…”

The influence of fermentation using marine yeast Hortaea werneckii SUCCY001 on antibacterial and antioxidant activity of Gracilaria verrucosa

“…Nowadays, bacterial infection have become a major challenge in the worldwide since the occurrence of multidrug resistant organisms (Prestinaci et al 2015). Hence, it is necessary to explore a new antibacterial agents from natural sources (Wijaya et al 2022). Besides, synthetic antioxidants including butylated hydroxyl toluene (BHT), and butylated hydroxyl anisole (BHA) have been extensively used to stabilize oils and fats in food products (Asif et al 2021;Viana da Silva et al 2021).…”