Antimicrobial activity of saquayamycins produced by Streptomyces spp. PAL114 isolated from a Saharan soil

Aouiche, Adel; Bijani, Christian; Zitouni, Abdelghani; Mathieu, Florence; Sabaou, Nasserdine

doi:10.1016/j.mycmed.2013.09.001

Cited by 31 publications

(15 citation statements)

References 22 publications

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“…PAL114 strain collected in Ghardaïa. MIC values of pure compounds were evaluated using conventional agar dilution method on a series of microorganisms, observing moderate activities, with the highest effects against Bacillus subtilis ATCC 6633 and Candida albicans M3 [94].…”

Section: Antimicrobialsmentioning

confidence: 99%

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

et al. 2019

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Actinobacteria, in particular “rare actinobacteria” isolated from extreme ecosystems, remain the most inexhaustible source of novel antimicrobials, offering a chance to discover new bioactive metabolites. This is the first overview on actinobacteria isolated in Algeria since 2002 to date with the aim to present their potential in producing bioactive secondary metabolites. Twenty-nine new species and one novel genus have been isolated, mainly from the Saharan soil and palm groves, where 37.93% of the most abundant genera belong to Saccharothrix and Actinopolyspora. Several of these strains were found to produce antibiotics and antifungal metabolites, including 17 new molecules among the 50 structures reported, and some of these antibacterial metabolites have shown interesting antitumor activities. A series of approaches used to enhance the production of bioactive compounds is also presented as the manipulation of culture media by both classical methods and modeling designs through statistical strategies and the associations with diverse organisms and strains. Focusing on the Algerian natural sources of antimicrobial metabolites, this work is a representative example of the potential of a closely combined study on biology and chemistry of natural products.

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Section: Antimicrobialsmentioning

confidence: 99%

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

et al. 2019

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“…19 Actinomycetes are Gram positive, filamentous bacteria with 55% of guanine and cytosine in their DNA. [20][21][22][23] Actinomycetes represent one of the most important classes of bacteria for their ability to produce a wide range of biologically active secondary metabolites, which are very effective against microbial pathogens. 20,23 More than 70-80% of all known antibiotics have been isolated from actinomycetes and are used in medicine and agriculture.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India

Ganesan¹,

Reegan²,

David³

et al. 2017

Alexandria Journal of Medicine

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Introduction: Microbial diseases are increasing year by year and they are becoming a big threat to public health. There are more than 200 known diseases transmitted by bacteria, fungi, viruses, prions, rickettsia and other microbes to humans. The emergence of drug resistance to chemical drugs is the biggest threat in controlling human pathogens. Hence novel antimicrobial agents from actinomycetes are timely needed for the control of several human pathogens. Aim: The aim was to find some actinomycetes with antimicrobial metabolites. Methods: Soil samples were collected from Nilgiris district in Western Ghats of Tamil Nadu, India. Actinomycetes were isolated using serial dilution and plating techniques on actinomycetes isolation agar. Streptomycin and ketoconazole (25 lg/disc) were used as reference controls. The active strains were identified by 16S rRNA and phylogenetic tree was constructed; the sequences were submitted in the GenBank. Results: Totally 106 actinomycete strains were isolated and cross streaked against various human microbial pathogens. Only 44 (41.50%) exhibited good antimicrobial activity against different pathogenic microbes. Five isolates (FMS-20, TGH-30, TGH-31, TGH-31-1 and IS-4) were chosen for secondary screening using filtrate. Among them FMS-20 filtrate showed good inhibition on the 16th day against all tested microbial pathogens. Further the intracellular methanol extract of FMS-20 showed maximum zone of inhibition against A. brasiliensis (22 mm) at 5 mg/disc. Similarly the extracellular ethyl acetate extract of FMS-20 showed maximum zone of inhibition against B. subtilis (25 mm).

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“…A previous work performed on a Saharan actinobacteria strain named PAL114 revealed the production of two bioactive molecules identified as saquayamycins A and C, known as anticancer agents (Aouiche et al 2014). In the present article, which is the continuation of a previously published work, the taxonomy of actinomycete strain and the production, purification, structure elucidation, and bioactivity of two other bioactive molecules produced by the same strain are described.…”

Section: Introductionmentioning

confidence: 64%

“…The actinobacteria strain PAL114 was isolated from a Saharan soil collected from Ghardaïa province, south Algeria (Aouiche et al 2014).…”

Section: Identification Of Actinobacteria Strain Pal114mentioning

confidence: 99%

Production of vineomycin A1 and chaetoglobosin A by Streptomyces sp. PAL114

et al. 2014

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An actinobacteria strain PAL114, isolated from a Saharan soil in Algeria, produces bioactive compounds. Morphological and chemical studies indicated that this strain belongs to the genus Streptomyces. Analysis of the 16S rRNA gene sequence showed a similarity level of 99.8 % with S. griseoflavus LMG 19344 T , the most closely related species. Two bioactive compounds, named P44 and P40, were extracted by dichloromethane from the cell-free supernatant broth and were purified by HPLC. Minimum inhibitory concentrations (MIC) of the compounds were determined against pathogenic and toxigenic microorganisms, most of which are multiresistant to antibiotics. The P40 fraction showed a strong activity especially against Candida albicans, Bacillus subtilis, and Staphylococcus aureus and has lower MIC values than those of P44 against most microorganisms tested. Chemical structures of compounds were determined based on spectroscopic and spectrometric analyses (UV-visible, mass, 1 H, and 13 C NMR spectra). The compounds P44 and P40 were identified as vineomycin A1 and chaetoglobosin A, respectively. Vineomycin A1 is known to be produced by some Streptomyces species. However, chaetoglobosin A is known to be produced only by fungi belonging to the genera Chaetomium, Penicillium, and Calonectria. This is the first time that chaetoglobosin A, known for its antimicrobial, anticancer, and cytotoxic effects, is reported in prokaryotes.

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Antimicrobial activity of saquayamycins produced by Streptomyces spp. PAL114 isolated from a Saharan soil

Abstract: This is an author's version published in: http://oatao.univ-toulouse.fr/20296

Cited by 31 publications

References 22 publications

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

Antimicrobial activity of some actinomycetes from Western Ghats of Tamil Nadu, India

Production of vineomycin A1 and chaetoglobosin A by Streptomyces sp. PAL114

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