Antimicrobial activity of selected plant species and antibiotic drugs against Escherichia coli O157:H7

Abstract

Recent research has focused on natural plant products as alternative for disease control in both developed and developing countries. Medicinal plants can be a possible source for new potent antimicrobial agents to which pathogenic strains are not resistant. The present study was carried out to determine the in vitro antimicrobial activity of 14 plant species namely; Allium sativum, Aloe vera, Bryophyllum pinnatum, Cassia ocidentalis, Citrus sinensis, Euphorbia hirta, Mangifera indica, Myristica fragrans, Ocimium gratissimum, Piper guineese, Psidium guajava, Spermacoce verticilata, Vernonia amygdalina and Zingiber officinale and 3 antibiotic drugs namely; ampicillin, ciprofloxacin and streptomycin on Escherichia coli O157:H7 isolated from human clinical sample. The extracts of the plant species were prepared by cold percolation method using ethanol and water as solvents. Phytochemical analyses of the extracts of the different plant species were determined using standard methods. Agar well diffusion method was used to evaluate the antimicrobial sensitivity test of the plant extracts and that of antibiotic drugs at different concentrations ranging from 31.25 to 500 mg/ml. The minimum inhibitory concentration (MIC) of the antimicrobial agents against Escherichia coli O157:H7 was also conducted. Phytochemical analyses of the plant species revealed the presence of bioactivity principle such as alkaloids, balsam, cardiac glucoside, flavonoids, phenols, resins, saponins, tannins, terpenes and steroids. The results showed that all the antimicrobial agents exhibited inhibitory effects against the growth of the bacterial isolate at various degrees. Among the plant species employed in the study, the ethanolic and water extracts of P. guajava showed the highest inhibitory effect against the bacterium with growth inhibition mean zone diameters of 29.9 and 26.0 mm respectively at 500 mg/ml. Following P. guagava in order of inhibitory effect against E. coli O157:H7 are ethanolic extracts A. sativum, Z. officinale, V. amygdalina and M. indica with mean zones of inhibition of 21.2, 20.8, 20.3 and 19.9 mm respectively at 500 mg/ml. The results also revealed that of the three antibiotic drugs used in the study, ciprofloxacin exhibited the highest inhibitory effect against the organism with zone of inhibition of 38.6 mm, followed by streptomycin 30.2 mm, while ampicilin had the least 22.3 mm. The MIC results reveal that some of the plant species showed similar inhibitory effect against the bacterium, while the MIC results of the rest of the plants varied from one another. The in vitro study of the antimicrobial activity of the extracts of the various plant species and that of the antibiotic drugs against E. coli O157:H7 has demonstrated that certain folk medicine can be as effective as modern medicine in combating pathogenic microorganisms.

Use of probiotics and botanical extracts to improve ruminant production in the tropics: A review

“…These compounds have been observed to exert specific anti-microbial effects against some pathogenic organisms. Itelima et al. (2017) examined the effects of some selected plant species against Escherichia coli 0157:H7 and it was observed that the extracts of Psidium guajava ( P. guajava L.), which possesses appreciable quantities of alkaloids, flavonoids, tannins, resins and trace amount of saponins, terpenes steroids and phenols had higher diameter of inhibition zone (29.9 mm) than ampicillin (22.3 mm) at the concentration of 500 mg/mL.…”

Shiga toxin-producing Escherichia coli O157:H7 among diarrheic patients and their cattle in Amhara National Regional State, Ethiopia

“…Sudan (5%) [45], plateau state, Nigeria (5%) [46], and France (3%) [47]. The relatively a higher prevalence of the organism found in the current finding might be because of a higher level of traditional breeding practice of animals and animal -to-human interaction in the study subjects.…”

“…However, our result was lower than those reported from Eastern Ethiopia (15.3%) [ 16 ], Eastern Cape Town, South Africa (17%) [ 36 ], and Maasai land Kenya (24.1%) [ 37 ]. On the contrary, this finding is higher than the studies reported from Gondar (1.9%) [ 38 ], Sebeta town, Ethiopia (3.2%) [ 13 ], Bishoftu town, Ethiopia (2.9%) [ 39 ], Debre Berhan cities, Ethiopia (0%) [ 19 ], Nairobi Kenya (0.2%) [ 40 ], Southern Mozambique (1.9%) [ 41 ], Morogoro Tanzania (3.6%) [ 42 ], Burkina Faso (9.67%) [ 43 ], Chattogram, Bangladesh (1.45%) [ 44 ], Sudan (5%) [ 45 ], plateau state, Nigeria (5%) [ 46 ], and France (3%) [ 47 ]. The relatively a higher prevalence of the organism found in the current finding might be because of a higher level of traditional breeding practice of animals and animal -to- human interaction in the study subjects.…”

Amperometric Biosensors as an Analytical Tool in Food, Dairy and Fermentation Industries

“…E.coli 0157 is a pathogenic strain and can cause diseases that result in stomach cramps and gentle looseness of the bowels and more deadly complications like hemorrhagic colitis (ridiculous diarrhea), haemolytic uremic disorder (HUS, prompts to kidney failure), and thrombotic thrombocytopenic purpura (TTP). 83,84 Escherichia coli (E.coli) 0157 is one such bacterial species that brought about numerous principal outbreaks in the past. N. Dhull et al proposed the development of amperometric biosensors for the detection of E. coli O157.…”