Antimicrobial Activity of Silver Nanoparticles with Antibiotics Against Clinically Isolated Acinetobacter baumannii

Mohamedsalih, Pyman M.; Sabir, Dana Khdr

doi:10.24271/psr.11

Cited by 2 publications

(3 citation statements)

References 30 publications

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“…For the identification of the isolates, a PCR machine was utilized for amplifying and sequencing the 16S rRNA gene. In the beginning, the colony PCR method was used to extract the bacterial genome [18], accompanied by the Taq Polymerase (2×) (GeNet Bio) to amplify the gene by means of the following primers: 7F: 5 -AGA GTT TGA TYM TGG CTC AG-3 and 1501R: 5 -ACG GYT ACC TTG TTA CGA CTT-3 ) [19]. The operation of PCR started with a denaturation process at 95 • C for 5 min, 30 cycles of denaturation for 30 sec at 95 • C, annealing for 30 sec at 60 • C, and an extension at 72 • C for 2 min.…”

Section: Bacterial Identificationmentioning

confidence: 99%

“…Different bacterial species have shown different responses in dealing with biosynthesized Ag NPs [67]. In this study, Ag NPs at concentrations ranging between 0.2 The access numbers of the 16S rRNA bacterial genes that were used to create the phylogenetic sketch are written in the brackets.…”

Section: Antibacterial Susceptibility Testingmentioning

confidence: 99%

“…Different bacterial species have shown different responses in dealing with biosynthesized Ag NPs [67]. In this study, Ag NPs at concentrations ranging between 0.2 mg/mL and 12.8 mg/mL were used.…”

Section: Antibacterial Susceptibility Testingmentioning

confidence: 99%

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Antibacterial Activity of the Green Synthesized Plasmonic Silver Nanoparticles with Crystalline Structure against Gram-Positive and Gram-Negative Bacteria

Afandy¹,

Sabir²,

Aziz

2023

Nanomaterials

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Nanoparticles (NPs) have attracted considerable interest in numerous fields, including agriculture, medicine, the environment, and engineering. The use of green synthesis techniques that employ natural reducing agents to reduce metal ions and form NPs is of particular interest. This study investigates the use of green tea (GT) extract as a reducing agent for the synthesis of silver NPs (Ag NPs) with crystalline structure. Several analytical techniques, including UV-visible spectrophotometry, Fourier transform infrared (FTIR) spectroscopy, high-resolution transmission electron microscopy (HR-TEM), and X-ray diffraction (XRD), were used to characterize the synthesized Ag NPs. The results of UV-vis revealed that the biosynthesized Ag NPs exhibited an absorbance plasmonic resonance peak at 470 nm. According to FTIR analyses, the attachment of Ag NPs to polyphenolic compounds resulted in a decrease in intensity and band shifting. In addition, the XRD analysis confirmed the presence of sharp crystalline peaks associated with face-centered cubic Ag NPs. Moreover, HR-TEM revealed that the synthesized particles were spherical and 50 nm in size on average. The Ag NPs demonstrated promising antimicrobial activity against Gram-positive (GP) bacteria, Brevibacterium luteolum and Staphylococcus aureus, and Gram-negative (GN) bacteria, Pseudomonas aeruginosa and Escherichia coli, with a minimal inhibitory concentration (MIC) of 6.4 mg/mL for GN and 12.8 mg/mL for GP. Overall, these findings suggest that Ag NPs can be utilized as effective antimicrobial agents.

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Section: Bacterial Identificationmentioning

confidence: 99%

Section: Antibacterial Susceptibility Testingmentioning

confidence: 99%

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Antibacterial Activity of the Green Synthesized Plasmonic Silver Nanoparticles with Crystalline Structure against Gram-Positive and Gram-Negative Bacteria

Afandy¹,

Sabir²,

Aziz

2023

Nanomaterials

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Fabricated silver nanoparticles by a combination of cell-free supernatant of Fusarium solani and Comamonas aquatica and its antibacterial activity

Fattah¹,

Arif²,

Hamzah³

2021

JZS

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The current study involves silver nanoparticles (AgNPs) synthesis, characterization, and antimicrobial activity of nanoparticles produced by a combination of cell-free supernatant (C-FS) of the intimate organisms, Fusarium solani and Comamonas aquatica as synthesis catalysts against Gram-negative and positive human pathogens. The detailed characterization of the Ag NPs was carried out using UV-visible spectroscopy, field emission Scanning Electron Microscopy (FE-SEM), transmission electron microscopy (TEM), and atomic force microscopy (AFM). From the UV-visible spectroscopy, the absorption peak was found at 442 nm, and FE-SEM images confirmed the formation of AgNPs. Further, TEM and AFM analysis demonstrated that fabricated AgNPs were relatively monodispersed, approximately spherical, and of the size between 2.0 - 7.5 nm. Furthermore, the antibacterial activity of AgNPs was determined by the agar well diffusion method, and results showed that AgNPs exhibited excellent antimicrobial activity against Gram-negative (E. coli, Pseudomonas aeruginosa, Salmonella enterica) and Gram-positive (Enterococcus faecalis and Staphylococcus aureus). Finally, The MIC test was performed to test the inhibitory concentration of AgNO3 against the bacteria under investigation. This is the first study proposing alternative sources to form AgNPs via synergistic metabolites of F. solani and C. aquatica. The results here offer a foundation for developing an effective therapy using AgNPs against various microorganisms which can endanger human beings.

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Antimicrobial Activity of Silver Nanoparticles with Antibiotics Against Clinically Isolated Acinetobacter baumannii

Cited by 2 publications

References 30 publications

Antibacterial Activity of the Green Synthesized Plasmonic Silver Nanoparticles with Crystalline Structure against Gram-Positive and Gram-Negative Bacteria

Antibacterial Activity of the Green Synthesized Plasmonic Silver Nanoparticles with Crystalline Structure against Gram-Positive and Gram-Negative Bacteria

Fabricated silver nanoparticles by a combination of cell-free supernatant of Fusarium solani and Comamonas aquatica and its antibacterial activity

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