Antimicrobial activity of the leaf extracts of &lt;i&gt;Moringa oleifera&lt;/i&gt; and &lt;i&gt;Jatropha curcas&lt;/I&gt; on pathogenic bacteria

Okorondu, SI; Akujobi, CO; Okorondu, JN; Anyado-Nwadike, SO

doi:10.4314/ijbcs.v7i1.

Cited by 8 publications

(4 citation statements)

References 7 publications

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“…The screening revealed that S. aureus , S. pyogenes , P. aeruginosa , E. coli , and N. gonorrhoeae were all resistant to the extracts. Ojiako (2014) [ 28 ] and Okorondu et al (2013) [ 29 ] previously found that M. oleifera leaf extracts were potent against E. coli and S. aureus when extracted with both the non-polar solvent hexane and the polar solvent ethanol. However, no action against the organisms was found in the current investigation.…”

Section: Resultsmentioning

confidence: 99%

Antioxidant and Antimicrobial Evaluations of Moringa oleifera Lam Leaves Extract and Isolated Compounds

2023

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Moringa oleifera, native to India, grows in tropical and subtropical regions around the world and has valuable pharmacological properties such as anti-asthmatic, anti-diabetic, anti-inflammatory, anti-infertility, anti-cancer, anti-microbial, antioxidant, and many more. The purpose of this study was to assess the free radical scavenging ability of two extracts and two pure compounds of M. oleifera Lam (hexane, ethanol, compound E3, and compound Ra) against reactive oxygen species, as well as their reducing power and antimicrobial activities. Bioautography antioxidant assay, 2,2-diphenyl-1-picrylhydrazyl (DPPH), hydrogen peroxide (H2O2) free radical scavenging, and iron (iii) (Fe3+ to Fe2+) chloride reducing power assays were used to assess the extracts’ qualitative and quantitative free radical scavenging activities. Furthermore, the extract and the compounds were tested against both Gram-positive and Gram-negative bacterial strains suspended in Mueller–Hinton Broth. The extracts and pure compounds showed noteworthy antioxidant potential, with positive compound bands in the Rf range of 0.05–0.89. DPPH), H2O2, and Fe3+ to Fe2+ reduction assays revealed that ethanol extract has a high antioxidant potential, followed by compound E3, compound Ra, and finally hexane extract. Using regression analysis, the half maximal inhibitory concentration (IC50) values for test and control samples were calculated. Compound Ra and ethanol exhibited high antioxidant activity at concentrations as low as ≈0.28 mg/mL in comparison with n-hexane extract, compound E3, ascorbic acid, and butylated hydroxytoluene standards. The radical scavenging activity of almost all M. oleifera plant extracts against DPPH was observed at 0.28 mg/mL; however, the highest activity was observed at the same concentration for ascorbic acid and butylated hydroxytoluene (BHT) with a low IC50 value of 0.08 mg/mL and compound Ra and ethanol with a low IC50 of 0.4 mg/mL, respectively. The extracts and pure compounds of M. oleifera have little to no antibacterial potential. M. oleifera extracts contain antioxidant agents efficient to alleviate degenerative conditions such as cancer and cardiovascular disease but have little activity against infectious diseases.

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Section: Resultsmentioning

confidence: 99%

Antioxidant and Antimicrobial Evaluations of Moringa oleifera Lam Leaves Extract and Isolated Compounds

2023

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“…Flavonoid play a role as antibiotic, also tannin and saponin is a fenolic compound which has antibacterial activity. Flavonoids have been reported have antimicrobial activity under laboratory condition through destruction of microbial membrane [22]. So, the bioactive compounds found at M. oleifera seeds promote its potency as antibacterial agent.…”

Section: Resultsmentioning

confidence: 99%

Activity of Moringa oleifera seed ethanolic extract against E. coli

Nurafifah

Widyastuti

Minarti

2021

ASSET

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Bacterial contamination in our environment is worrying, mainly contamination at drinking waters, vegetables, foods, soil which close to our daily activity. The main contamination in environment is caused by E. coli which simply found easily surround us. So, it leads to bigger problem if not immediately solve. One of possible yet safe compounds to overcome this problem is the use of natural product such as M. oleifera seeds as antibacterial agents. This study want to find out the ability of M. oleifera seed ethanolic extract as antibacterial agent against E. coli. Ethanolic extract of M. oleifera seeds are concentrated into 25%, 50%, and 75%, then treated to E. coli culture under laboratory condition. The inhibitory zone diameter which formed after 24 hours incubation was measured and compared to control with no extract treatment. The result of this study showed that there is inhibitory zone formed in three groups of treatmen (T1-T3), but there is no inhibitory zone formed at control group. The 75% ethanolic extract of M. oleifera seeds (T3) has the wides inhibitory zone diameter among four groups, followed by the 50% extract (T2) and 25% extract (T1) with diameter 15,03 ± 0,55 mm; 11,00 ± 1,32 mm; 7,03 ± 0,90 mm, respectively. All inhibitory zone diameter among groups in this research statistically different with strong inhibitory status at T2 as well as T3, and moderate inhibitory status at T1.

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“…The increasing occurrence of antibiotic-resistant strains of bacteria and the spread of multidrug-resistance with reduced sensitivity to antimicrobial agents has compelled intensive searches for effective methods to overcome the therapeutic problem, as well as to discover new infectionfighting approaches [1]. One of the strategies to reduce the resistance to antimicrobial agents is to use antibiotic resistance inhibitors such as that found in traditional medicine [2]. Many plant-based agents used in traditional medicine are considered to be potential or actual therapeutic substitutes to antibiotics, and are considered natural, harmless and inexpensive, yet still effective [3].…”

Section: Introductionmentioning

confidence: 99%

Phytochemical analysis and evaluation of antibacterial activity of Moringa oleifera extracts against gram-negative bacteria: an in vitro and molecular docking studies

Ganjo

Adham

Aka

2022

Current Issues in Pharmacy and Medical Sciences

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Moringa oleifera seed and leaf are used traditionally for the treatment of various health problems (among others, hypertension, scrapes, skin infection, diabetes, genitourinary illnesses), and to boost the immune system, as well as to act as a contraceptive. In this study, the antibacterial activity of seed and leaf M. oleifera extracts on three-gram negative bacteria was investigated, and phytochemical analysis for the association of antibacterial activity with the active constituents in the plant was determined. Moreover, understanding of the mechanism of action was achieved by applying the Auto Dock Vina technique. The phytochemical screening of M. oleifera seed and leaf extracts exhibited the presence of alkaloids, carbohydrates, cardioactive glycosides, flavonoids, tannins, phenols, steroids and terpenoids. In silico results revealed that compounds (4-O-caffeoyl quinic acid, 4-(α-L-rhamnopyranosyloxyl)-benzylisothiocyanate); (Isoquercitrin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate); and (Astragalin, 4-(α-L-rhamnopyranosyloxy) benzyl glucosinolate) from leaf and seed have the highest binding affinity and very good interactions with Transcriptional Activator Protein (LasR), Klebsiella pneumoniae carbapenemase (KPC), and Malonyl-CoA-acyl carrier protein transacylase (FabD), respectively.

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Antimicrobial activity of the leaf extracts of <i>Moringa oleifera</i> and <i>Jatropha curcas</I> on pathogenic bacteria

Cited by 8 publications

References 7 publications

Antioxidant and Antimicrobial Evaluations of Moringa oleifera Lam Leaves Extract and Isolated Compounds

Antioxidant and Antimicrobial Evaluations of Moringa oleifera Lam Leaves Extract and Isolated Compounds

Activity of Moringa oleifera seed ethanolic extract against E. coli

Phytochemical analysis and evaluation of antibacterial activity of Moringa oleifera extracts against gram-negative bacteria: an in vitro and molecular docking studies

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