Antimicrobial agents from Bletilla striata

Dihydrophenanthrenes from Bletilla formosana

“…The ethyl acetate and n-butanol-soluble fractions were separatedly subjected to silica gel column chromatography. Fractions rich in polyphenolic compounds were combined and purified by Sephadex LH-20 column to give three new dihydrophenanthrenes, 4-methoxy-9,10-dihydrophenanthrene-1,2,7-triol (1), 1-(4-hydroxybenzyl)-4,7-dimethoxy-9,10-dihydrophenanthrene-2-ol (2), and 1,3,6-tri(4-hydroxylbenzyl)-4-methoxydihydrophenanthrene-2,7-diol (3), along with seven known phenanthrene derivatives, 1-(4-hydroxybenzyl)-4-methoxy-9,10-dihydrophenanthrene-2,7-diol (4), 3) 1,6-di(4-hydroxylbenzyl)-4-methoxy-9,10-dihydrophenanthrene-2,7-diol (5), 4) 1,3-di(4-hydroxybenzyl)-4-methoxy-9,10-dihydrophenanthrene-2,7-diol (6), 3,4) 1-(4-hydroxybenzyl)-4-methoxyphenanthrene-2,7-diol (7), 4) 1-(4-hydroxybenzyl)-4,8-di-methoxyphenanthrene-2,7-diol (8), 5) 1,8-bi(4-hydroxybenzyl)-4-methoxyphenanthrene-2,7-diol (9), 6) and blestriarene B (10), 7) a bibenzyl, 2Ј,6Ј-bis(p-hydroxybenzyl)-3,3Ј-dihydroxy-5-methoxybibenzyl (11), 8) six known flavonoids, 8-C-p-hydoxybenzylkaempferol (12), 9) apigenin (13), 10) 6-methoxykaempferol (14), 11) kaempferol (15), 12) isorhamnetin (16), 13) kaempferol 7-O-glucoside (17), 12) 3,4-dihydroxybenzaldehyde (18), 14) protocatechuic acid (19), 12) p-(hydroxymethyl)phenyl-b-D-glucoside (20). 15) Compound 1 was isolated as a pale yellow amorphous powder with a molecular formular C 15 .…”

New Polyphenols and Triterpene from the Pseudobulbs of Pleione Formosana

“…The EtOAc and n-BuOH-soluble fractions were separatedly subjected to silica gel column chromatography (CC). Fractions rich in polyphenolic compounds were further separated and purified on Sephadex LH-20 column followed by C 18 CC and semipreparative RP-18 HPLC to give a new dihydrophenanthrene, pleioanthrenin (1), two bibenzyls, pleiobibenzynin A (2) and B (3), and a cycloartane triterpenoid, (24R)-cyclomargenyl p-coumarate (4), together with 1-(4-hydroxybenzyl)-4,7-dimethoxy-9,10-dihydrophenanthrene-2-ol (5), 3 bulbocol (6), 4 3¢,5-dihydroxy-2-(4-hydroxylbenzyl)-3-methoxybibenzyl (7), 5 bulbocodin C (8), 6 arundin (9), 7 3,3¢-dihydroxy-2,6-bis(4-hydroxybenzyl)-5-methoxybibenzyl (10), 8 2,6-bis(4-hydroxybenzyl)-3¢,5-dimethoxy-3-hydroxybibenzyl (11), 8 (24R)-cyclomargenol (12).…”

Chemical Constituents from the Aerial Parts of Cyrtopodium paniculatum

“…The alcoholic crude extract was suspended in water and sequentially extracted with cyclohexane, CH2Cl2 and n-BuOH. The CH2Cl2 extract was concentrated to dryness and subjected to silica gel column chromatography, Sephadex LH-20 and preparative thin layer chromatography (PTLC) to afford eight new compounds 1-8 ( Figure 1) together with 28 previously described compounds 9-35 which were identified as para-ethoxybenzyl alcohol (9) [18,19], 3,4,6-trimethoxyl-9,10-dihydrophenanthrene-2,7-diol (10) [20], confusarin (11) [21], erianthridin (12) [22], para-hydroxybenzaldehyde (13) [18], nudol (14) [23], cephathrene-B (15) [24], gigantol (16) [25], batatasin III (17) [26], ephemeranthoquinone (18) [27], coelonin (19) [28], 2,4-dimethoxyphenanthrene-3,7-diol (20) [29], lusianthridin (21) [30], densiflorol B (22) [31], denthyrsinin (23) [32], bleformins A (24) and B (25) [33], chrysoeriol (26) [34,35], vanillic alcool (27) [36], gastrodigenin (28) [37,38], 1-(4-hydroxybenzyl)-4-methoxyl-9,10-dihydrophenanthrene-2,7-diol (29) [39], shancidin (30) [40], blestriarenes A (32) and B (31) [41], velutin (33) [42], (+)-syringaresinol (34) [43] and (+)-balanophonin (35) [44], respectively, by comparison of their UV, MS, and NMR data with literature data.…”