Antimicrobial and anti-inflammatory activities, wound-healing effectiveness and chemical characterization of the latex of Jatropha neopauciflora Pax

Hernandez-Hernandez, Ana Bertha; Alarcón-Aguilar, Francisco Javier; Almanza-Perez, Julio César; Nieto-Yañez, Oscar; Olivares-Sanchez, J.M.; Durán‐Díaz, Ángel; Rodríguez-Monroy, Marco Aurelio; Canales-Martínez, María Margarita

doi:10.1016/j.jep.2017.04.003

Cited by 31 publications

(14 citation statements)

References 27 publications

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“…Similarly, diluted J. curcas latex has been shown to have healing properties, but undiluted latex has produced caustic lesions [8]. These findings contrast with another study in which the latex of J. neopauciflora Pax showed wound healing efficacy at 50%, 70%, and even 100% concentrations [15]; this contrasts with the data of Salas et al who reported caustic lesions [8] and our results. In summary, the above data suggest that the composition and concentration of phytochemicals can differ significantly among the latex of different Jatropha species.…”

Section: Discussioncontrasting

confidence: 99%

“…According to this broad analysis, it is likely that alkaloids were the phytochemical compounds responsible for wound healing activity because they were not detected in the chloroform fraction. Although the composition of latex is similar among Jatropha species, some phytochemicals are present at lower concentrations or completely absent depending on the specific Jatropha species [10,15,24,25]. The differences in latex phytochemical composition detected among different published papers may suggest that composition is dependent on the species of Jatropha species and the sensitivity of the analytical techniques used.…”

Section: Discussionmentioning

confidence: 97%

“…The lattices of Jatropha neopauciflora Pax species, which are endemic to Mexico, promote wound healing that can attain up to 100% of the tensile strength of undamaged skin in a murine model. These lattices also promote antibacterial activity without toxicity when tested in cell lines [15]. When the cells infiltrating the lesion site were characterized, it was noted that CD68+ and CD34+ cells mobilized to skin tissues treated with J. curcas Linn.…”

Section: Introductionmentioning

confidence: 99%

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The wound healing action of a cream latex formulation of Jatropha gaumeri Greenm. in a pre-clinical model

et al. 2020

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Background and Aim: Jatropha gaumeri Greenm. is commonly used to treat mouth blisters and skin rashes, its latex has analgesic and anti-inflammatory activity on buccal ulcer. This study aimed to demonstrate the wound healing activity of a cream formulation of Jatropha gaumeri Greenm. latex in a murine model, provide a histological assessment of its scarring effects, and identify the family of phytochemicals involved in these effects. Materials and Methods: Latex was obtained from the cut stalk leaves and young stems of J. gaumeri and stored in sterile tubes with protection from light. Chloroform, ethyl acetate, and aqueous fractions of the latex were obtained. Fifty male Balb/c mice aged 10-12 weeks were divided into 10 groups of five mice: Group 1 corresponded to healthy mice with wounds; Group 2 corresponded to mice with wounds and treated with A-Derma®; and from Group 3 to group 10 corresponded to mice treated with a different latex fraction. A circular skin wound of about 1 cm was made on the paravertebral region of each mouse under anesthetized and aseptic conditions. The wounds were topically treated every 24 h with the respective extracts for 22 days, after which skin tissue specimens were obtained and stained with hematoxylin-eosin and Masson's trichrome. The efficiency of healing was measured by quantifying the tensile strength of the scars. The phytochemicals in the latex were elucidated using thin chromatography. Results: The aqueous latex fraction produced the best wound healing activity and was superior to the positive control. Reepithelialization at the histological level resulted in tissue that resembled healthy skin in terms of the appearance of collagen, the regeneration of hair follicles, and cellularity of the dermis, which showed organized epithelialization. A wound healing efficacy of 97% was observed, and it seems that alkaloids were the phytochemicals mostly likely responsible for these effects. Conclusion: J. gaumeri latex exhibited wound healing activity, possibly mediated by phytochemicals such as alkaloids in the aqueous fraction.

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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The wound healing action of a cream latex formulation of Jatropha gaumeri Greenm. in a pre-clinical model

et al. 2020

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“…All latex of Jatropha showed the antibacterial activities against MRSA and CRPA in the diffusion method (20.4–23.7 mm and 12–15 mm), MIC (0.19–6.25% and 25%), MBC (0.39–12.5% and 50%). These results are following the research report by Hernandez-Hernandez [ 13 ] that the latex of Jatropha neopauciflora Pax demonstrated inhibition against S. aureus . In another study, the latex of Jatropha curcas displayed potent antimicrobial activity against Pseudomonas aeruginosa [ 14 ].…”

Section: Discussionsupporting

confidence: 90%

Antibacterial Potential of Jatropha sp. Latex against Multidrug-Resistant Bacteria

Prastiyanto

Tama

Ananda

et al. 2020

International Journal of Microbiology

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Objective. This study was aimed to evaluate the antibacterial activity of the latex of three species members of Jatropha (J. curcas, J. gossypilofia Linn., and J. multifida) against methicillin-resistant Staphylococcus aureus (MRSA), extended-spectrum beta-lactamase- (ESBL-) producing Escherichia coli and ESBL-producing Klebsiella pneumonia, carbapenemase-resistant Enterobacteriaceae (CRE)-E. coli, K. pneumoniae-carbapenemase (KPC), and carbapenemase-resistant Pseudomonas aeruginosa (CRPA). Method. The antibacterial activities were calculated based on the inhibition zones using the Mueller–Hinton agar diffusion method, minimum inhibitory concentration (MIC) using Mueller–Hinton broth in a microdilution method, and minimum bactericidal concentration (MBC) using blood agar plate. Results. The latex of Jatropha showed antibacterial activities against the MRSA and CRPA. All latex of Jatropha appeared to have the antibacterial activities against MRSA and CRPA in the diffusion method (20.4–23.7 mm and 12–15 mm), MIC (0.19–6.25%, and 25%), and MBC (0.39–12.5% and 50%). Phytochemical screening of latex indicated the presence of flavonoids. Conclusions. The latex of J. curcas, J. gossypilofia Linn., and J. multifida has the potential to be developed as antibacterial agents, especially against MRSA and CRPA strain, but further in vivo research and discovery of the mode of its action are required to shed the light on the effects.

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“…Tensile strength of all samples were evaluated by a biomechanical module (1 mm/s tensile stretching speed, 5 g/s stretching force, 2.5 kg max force) compatible with BIOPAC MP30 Acq. [27,28].…”

Section: Biomechanical Studymentioning

confidence: 99%

Evaluation of the Wound Healing Properties of Luteolin Ointments on Excision and Incision Wound Models in Diabetic and Non-Diabetic Rats

Özay¹,

Güzel²,

Erdoğdu³

et al. 2018

Rec.Nat.Prod.

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Luteolin, which is structurally classified under the flavonoids, is naturally distributed abundant among medicinal plants, vegetables and fruits. The wound healing effects of Luteolin ointments were evaluated for incision and excision wound models on non-diabetic and Streptozotocin induced diabetic rats by using macroscopical, biomechanical, biochemical and histopathological methods. Two different concentrations (0.5 % and 1 % (w/w)) of the Luteolin ointment were prepared with the mixture of glycol stearate:propylene glycol:liquid paraffin (3:6:1) and 0.5 g of the ointments were applied topically on wounds once daily for 7 and 14 days. During the experiments, wounds were visually observed, photographically documented and wound areas were measured. After 7 and 14 days treatment, animals were sacrificed under anesthesia and hydroxyproline measurement and biomechanical analysis were performed. Histopathology of the wound area was evaluated considering features of re-epithelialization, thickness of the granulation tissue, angiogenesis, presence of inflammation, number of mast cells. Outcomes of this study revealed that Luteolin ointments improved wound healing process of skin tissue both in non-diabetic and diabetic wounds. The best wound healing activity was observed in incision and excision wounds (97.6 %, 96.1 %, respectively) treated with 0.5 % (w/w) Luteolin ointment on day 14 according to macroscopic results.

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Antimicrobial and anti-inflammatory activities, wound-healing effectiveness and chemical characterization of the latex of Jatropha neopauciflora Pax

Cited by 31 publications

References 27 publications

The wound healing action of a cream latex formulation of Jatropha gaumeri Greenm. in a pre-clinical model

The wound healing action of a cream latex formulation of Jatropha gaumeri Greenm. in a pre-clinical model

Antibacterial Potential of Jatropha sp. Latex against Multidrug-Resistant Bacteria

Evaluation of the Wound Healing Properties of Luteolin Ointments on Excision and Incision Wound Models in Diabetic and Non-Diabetic Rats

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