Antimicrobial and antibiofilm efficacy of a copper/calcium hydroxide-based endodontic paste against &lt;i&gt;Staphylococcus aureus&lt;/i&gt;, &lt;i&gt;Pseudomonas aeruginosa&lt;/i&gt; and &lt;i&gt;Candida albicans &lt;/i&gt;

Gomes

2019

JoF

The ability to form biofilms is a common feature of microorganisms, such as bacteria or fungi. These consortiums can colonize a variety of surfaces, such as host tissues, dentures, and catheters, resulting in infections highly resistant to drugs, when compared with their planktonic counterparts. This refractory effect is particularly critical in polymicrobial biofilms involving both fungi and bacteria. This review emphasizes Candida spp.-bacteria biofilms, the epidemiology of this community, the challenges in the eradication of such biofilms, and the most relevant treatments.

Section: Oral Disease Managementmentioning

confidence: 99%

Candida spp./Bacteria Mixed Biofilms

Gomes

2019

JoF

“…E. faecalis and S. aureus are Gram-positive cocci, facultative anaerobes that have a great potential to form biofilms on different substrates; this ability has been associated with their persistence after primary endodontic treatment on numerous occasions [ 4 , 9 , 15 , 16 ]. The organization of bacteria in biofilms, and dynamic and complex environments provides increased resistance against the elements intended for their eradication, compromising their effectiveness [ 2 , 15 , 16 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Development of a New Sponge-Based Delivery System for Intracanal Antimicrobial Administration in Endodontic Treatment

Ruíz‐Piñón

Gancedo-Gancedo

Prado

et al. 2021

JCM

This study aimed to evaluate the in vitro performance of collagen-based sponges as a drug delivery system for intracanal antimicrobial administration. Four groups of loaded collagen-based sponges (A, 0.3% w/v amoxicillin trihydrate: potassium clavulanate (4:1); B, 0.03% w/v chlorhexidine gluconate [CHX]; C, 0.3% w/v amoxicillin trihydrate: potassium clavulanate (4:1) and 0.03% w/v CHX; D, 1% w/v amoxicillin trihydrate: potassium clavulanate (4:1) and 0.03% w/v CHX) were designed. Release kinetics were tested in vitro on cultures in Petri dishes, and the effect on bacterial biofilms was studied ex vivo on 114 extracted human single-rooted teeth. Biofilm formation was tested by scanning electron microscopy (SEM). Collagen sponges containing amoxicillin and chlorhexidine showed a time-sustained antimicrobial effect in vitro and were also able to destroy mature biofilms ex vivo. This datum was validated by means of SEM-based study of E. faecalis and S. aureus biofilms.

“…Recently, we provided in vitro evidence on the efficacy of Cupral as antimicrobial compound, being effective both against planktonic and biofilm communities. 16 Moreover, clinical evidence exists on the successful treatment of periodontal disease patients by Cupral. 17 18 Here, we show that Cupral can be successfully employed to perform aligners hygiene; microbial contamination is totally removed, even when Cupral is diluted up to 1.25%.…”

Section: Discussionmentioning

confidence: 99%

“…Cupral (a copper–calcium–hydroxide based compound) is a dental paste clinically used in periodontal infections, being endowed with antimicrobial activity. 15 16 Moreover, recent clinical experience demonstrates that Cupral efficiently counteracts endodontic infections. 17 18 Yet, its mechanisms of action have not been investigated; neither data exist on Cupral use in other dentistry fields.…”

Section: Introductionmentioning

confidence: 99%

Efficacy of a Copper–Calcium–Hydroxide Solution in Reducing Microbial Plaque on Orthodontic Clear Aligners: A Case Report

et al. 2019

Self Cite

The aim of this study was to investigate the ability of a copper–calcium–hydroxide-based compound to remove microbial plaque naturally produced onto orthodontic clear aligners. A commercially available dental paste, named Cupral, based on copper–calcium–hydroxide, was used. A healthy volunteer (female, 32 years old), undergoing orthodontic treatment with thermoplastic clear aligners was enrolled. By conventional/confocal microscopy and colony-forming unit (CFU) assay, 2-week used aligners were examined for microbial plaque, prior and following exposure to Cupral. Confocal microscopy revealed abundant plaque irregularly distributed onto the aligner surface. Following Cupral treatment, a drastic decrease occurred in plaque thickness and matrix presence. As assessed by the CFU assay, total microbial load approached 109 CFUs/aligner, with slight differences in aerobiosis and anaerobiosis culture conditions; six macroscopically different types of colonies were detected and identified by matrix-assisted laser desorption ionization-time of flight mass spectrometry. Following Cupral treatment, microbial load dropped to undetectable levels, irrespectively of the conditions considered. Exposure of clear aligners to Cupral results in the elimination of contaminating microorganisms; the antimicrobial activity is retained up to 1.25% concentration. Overall, our data describe a novel use of Cupral, a copper–calcium–hydroxide-based compound, in daily hygiene practices with promising results.