Antimicrobial and antinematicidal metabolites from Streptomyces cuspidosporus strain SA4 against selected pathogenic bacteria, fungi and nematode

Sholkamy, Essam Nageh; Muthukrishnan, P.; Abdel-Raouf, Neveen; Nandhini, X.; Ibraheem, Ibraheem Borie Mohammad; Mostafa, Ashraf Abdel‐Fattah

doi:10.1016/j.sjbs.2020.08.043

Cited by 28 publications

(15 citation statements)

References 38 publications

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“…Studies have already demonstrated actinomycetes metabolites active against bacteria (27), fungi (28), viruses (29), nematodes (30), and insects (31), as well as compounds with antitumor (32) and herbicide (33) activities. Some species can also produce enzymes of industrial interest, such as chitinases, cellulases, and amylases (34), and enzymes involved in plant growth promotion, like phosphatases and nitrogenases (35).…”

Section: Actinomycetes: a Rich Source Of Bioactive Compoundsmentioning

confidence: 99%

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

et al. 2022

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The use of fertilizers and chemical pesticides promotes significant improvements in crop development, but some problems and risks associated with them limit their application. An alternative is using biological inputs based on microorganisms, increasing production while combining efficiency and sustainability. Actinomycetes are a group of bacteria belonging to the phylum Actinobacteria, recently re-named Actinobacteriota. They represent important microbial communities in the soils with increasing agricultural applications, especially in the biological control of insect-pest and plant disease and in plant growth promotion. Studies report their promising use as microbiological inoculants by exploring mechanisms to improve plant development, such as biological nitrogen fixation, phosphate solubilization, production of phytohormones, and other biocompounds. In addition, many species produce metabolic pathways that generate high-value antibiotics, extracellular enzymes and secondary metabolites other than antimicrobials, with potential in the control of phytopathogenic fungi, insects, and nematodes. These actinomycetes could be used to formulate novel bioinoculants composed of spores and/or mycelium. Considering that the research in this field is up-and-coming, with significant economic and environmental impacts in the future, this review aims to group the most relevant works that explore the biodiversity of actinomycetes, helping to develop inoculants and biodefensives for more productive and conscious agriculture.

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Section: Actinomycetes: a Rich Source Of Bioactive Compoundsmentioning

confidence: 99%

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

et al. 2022

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“…A large number of acid compounds such as chloroacetic acid, furandicarboxylic acid, formic acid, pentacosanoic acid, hexadecanoic acid, and benzenedicarboxylic acid were a main type of antifungal production. Benzenedicarboxylic acid possessing high peak area is a main metabolite of S. cuspidosporus with high antagonistic activity against pathogenic bacteria, fungi, and nematode ( Sholkamy et al, 2020 ). Chloroacetic acid and hexadecanoic acid had the potential for controlling Colletotrichum gloeosporioides ( Rajaofera et al, 2019 ).…”

Section: Discussionmentioning

confidence: 99%

Biocontrol Potential of Endophytic Streptomyces malaysiensis 8ZJF-21 From Medicinal Plant Against Banana Fusarium Wilt Caused by Fusarium oxysporum f. sp. cubense Tropical Race 4

et al. 2022

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Banana (Musa spp.) is an important fruit crop cultivated in most tropical countries. Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is the most destructive fungal disease. Biocontrol using endophytic microorganisms is considered as a safety and sustainable strategy. Actinomycetes have a potential for the production of diverse metabolites. Isolation of endophytic actinomycetes with high efficiency and broad-spectrum antagonism is key for exploring biocontrol agents. Our previous study showed that a total of 144 endophytic actinomycetes were isolated from different tissues of medicinal plants in Hainan, China. Especially, strain 8ZJF-21 exhibited a broad-spectrum antifungal activity. Its morphological, physiological, and biochemical characteristics were consistent with the genus Streptomyces. The phylogenetic tree demonstrated that strain 8ZJF-21 formed a distinct clade with Streptomyces malaysiensis. Average nucleotide identity (ANI) was 98.49% above the threshold of novel species. The pot experiment revealed that endophytic Streptomyces malaysiensis 8ZJF-21 could improve the plant resistance to Foc TR4 by enhancing the expression levels of defense-related and antioxidant enzyme genes. It also promoted the plant growth by producing several extracellular enzymes and metabolites. Antifungal mechanism assays showed that S. malaysiensis 8ZJF-21 extract inhibited mycelial growth and spore germination of Foc TR4 in vitro. Pathogenic cells occurred cytoplasmic heterogeneity, disappeared organelles, and ruptured ultrastructure. Sequencing and annotation of genome suggested that S. malaysiensis 8ZJF-21 had a potential of producing novel metabolites. Nineteen volatile organic compounds were obtained from the extract by Gas Chromatography-Mass Spectrometry (GC-MS). Hence, endophytic Streptomyces strains will become essential biocontrol agents of modern agricultural practice.

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“…Antimicrobial Resistance (AMR) of pathogenic bacteria emerges when the microorganisms acquire resistance to antibiotics, enabling them to no longer respond to antibiotics [38]. Crude extract from M1 and M3 isolates were screened for antibacterial activity against ten bacterial pathogens, and two fungus strains, using the agar well diffusion method [39] (6 mm). As previously known, fungal pathogens have been responsible for significant agricultural losses caused by the plant diseases they produce; they are also the origin of many medically related illnesses [40].…”

Section: Determination Of Anti-microbial Property By Agar Cup Methodsmentioning

confidence: 99%

Streptomyces peucetius M1 and Streptomyces lavendulae M3 Soil Isolates as a Promising Source for Antimicrobials Discovery

Jha¹,

Jain²,

Nikumb³

et al. 2022

JPRI

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The ever-increasing fatality due to the surge of drug resistance has demanded the development of therapeutic metabolites with novel modes of mechanism. Despite the discovery of new antimicrobials, antibiotic resistance is increasing at an alarming rate. The emergence of novel molecules is necessary for targeting the Multidrug (MDR) and Pan-Drug resistant (PDR) pathogens which are the principal cause of life-threatening infections. Streptomyces strains commonly found in the soil produce secondary metabolites which have outstanding potential for being an antifungal and antibacterial agent. This study aims to isolate, identify, and characterize Streptomyces strains isolated from soil and extract their crude metabolites to determine their hidden therapeutic property. Out of four isolates identified, Streptomyces peucetius and Streptomyces lavendulae have shown significant antimicrobial activity against test organisms. To further determine the activity of their extracts GC-MS analysis, and MIC, as well as radical scavenging activity, were determined. GC-MS analysis revealed the presence of compounds 2,3-butanediol, 1-methyl-2-pyrrolidone-4-carboxamide, and L-Lactic acid which are frequently associated with potent antimicrobial nature. The most vulnerable pathogen to M1 extract was Candida albicans with a MIC value of 7.8125 μg/mL while the susceptible bacteria to M3 extract included Staphylococcus aureus with a MIC value of 3.90 μg/mL. The data obtained for antioxidant activity in the case of metabolic extracts M1 and M3 had an IC50 value of 43.17 µg/mL and 22.65 µg/mL respectively. The above findings hint at the unrealized potential of Streptomyces strains.

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Antimicrobial and antinematicidal metabolites from Streptomyces cuspidosporus strain SA4 against selected pathogenic bacteria, fungi and nematode

Abstract: Graphical abstract

Cited by 28 publications

References 38 publications

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

The Potential Use of Actinomycetes as Microbial Inoculants and Biopesticides in Agriculture

Biocontrol Potential of Endophytic Streptomyces malaysiensis 8ZJF-21 From Medicinal Plant Against Banana Fusarium Wilt Caused by Fusarium oxysporum f. sp. cubense Tropical Race 4

Streptomyces peucetius M1 and Streptomyces lavendulae M3 Soil Isolates as a Promising Source for Antimicrobials Discovery

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