Antimicrobial and antioxidant activities of secondary metabolites from endophytic fungus Botryosphaeria fabicerciana (MGN23-3) associated to Morus nigra L

Abstract

This study was to evaluate the biological activity of the extract of Botryosphaeria fabicerciana isolated from leaves of Morus nigra. The volatile compounds from the crude extract were analyzed by GC-MS which demonstrate that mellein and β-orcinaldehyde were are the major compounds. The best minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract was observed against Gram-positive bacteria, with a MIC of 15.6 μg/mL towards B. cereus and MIC of 62.5 μg/mL towards S. aureus and B. subtilis. MBC values of 31.25 μg/mL, 62.5 μg/mL, and 250 μg/mL were observed towards B. cereus, B. subtilis, and S. aureus, respectively. The cytotoxicity analyses showed CC50 of 115 μg/mL. The crude extract showed antioxidant activity by the DPPH, ABTS, and FRAP assays. Therefore, the extract of the endophytic fungus presented biotechnological potential as an antibacterial and antioxidant agent.

Bioactivity of Endophytic Fungi Isolated from Branch of Jambu Mawar (Syzygium jambos (L.) Alston)

“…B. mamane also contains sesquiterpen (Oliveira et al, 2015), thiodiketopiperazines (botryosulfuranols A-C) (Barakat et al, 2019), botryomaman, 2,4-dimethoxy-6-pentylphenol, (R)-(-)-mellein, primin, cis-4hydroxymellein, trans-4-hydroxymellein and 4,5dihydroxy-2-hexenoic acid which show antibacterial properties against S. aureus (Pongcharoen et al, 2007). B. fabicerciana contains mellein and borcinaldehyde compounds, which are useful against B. cereus, S. aureus, B. subtilis bacteria and have antioxidants properties (Silva et al, 2021). Isocumarin and tryptamin are antifungals produced by B. ramose (Hu et al, 2020;Wu et al, 2019).…”

Antimicrobial and Antioxidant Activities of Endophytic Fungi Associated with Arrabidaea chica (Bignoniaceae)

“…Other species of Botryosphaeria isolated as endophytes also have antimicrobial potential. Silva et al [ 22 ] evaluated the antimicrobial activity of metabolites from B. fabicerciana that were isolated from Morus nigra L. and observed an MIC of 64 µg/mL for S. aureus , and an MIC of 1000 µg/mL for E. coli . Xiao et al [ 57 ] identified 17 of the metabolites produced by B. dothidea .…”

“…In recent decades, endophytic fungi have gained prominence as a rich source of natural compounds with interesting pharmacological activities [ 13 , 14 , 15 ]. Therefore, exploring endophytic fungi that inhabit medicinal plants provides ample opportunities to discover new metabolites with potential bioactivity [ 16 , 17 , 18 , 19 , 20 , 21 , 22 ]. In addition, investigations regarding endophytes from tropical plants are still limited, especially when considering the pharmacological potential of these isolates [ 23 ].…”

Endophytic Fungi as a Source of Antiviral Compounds – A Review

“…In previous studies, we reported the potential of endophytes against several types of microorganisms, including protozoa [7] and bacteria, [8] and described the main compounds produced, such as linoleic acid, [7] mellein and β-orcinaldehyde, [44,45] kojic acid, α-cyclopiazonic acid, and 20,25-dihydroxyaflavinine. [46] Thus, the study of the endophyte-host relationship can establish alternative sources of substances of biotechnological interest.…”