Antimicrobial and cytotoxic activities of endophytic fungi isolated from Piper crocatum Ruiz &amp; Pav

Astuti, Puji; Wahyono, Wahyono; Nababan, Octavian Ashido

doi:10.12980/apjtb.4.2014apjtb-2014-0073

Cited by 24 publications

(21 citation statements)

References 22 publications

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“…isolated from Cinnamomum mollissimum offered potent cytotoxicity against murine leucemia cells (Santiago et al 2012). Also, the ethyl acetate extract of endophytic fungi isolated from P. crocatum and red alga Bostrychia tenella inhibited the growth of different tumour cell lines with IC 50 value of 37.43 (T47D) and 1 lg/mL (SF-295), respectively (Astuti et al 2014;de Felicio et al 2015). It was also noted that EAC cells without the treatment of extract had no cytotoxicity effect in the present study.…”

Section: Anticancer and Dna Fragmentation Assaysupporting

confidence: 56%

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Murali

Mahendra

Hema

et al. 2017

Pharmaceutical Biology

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Context: Sida acuta Burm.f. (Malvaceae) extracts are reported to have applications against malaria, diuretic, antipyretic, nervous and urinary diseases. No fungal endophytes of S. acuta are reported. Objective: Isolation, identification and evaluation of antibacterial, antioxidant, anticancer and haemolytic potential of fungal endophytes from the ethnomedcinal plant S. acuta. Materials and methods: Sida acuta stem segments were placed on PDA medium to isolate endophytic fungi. The fungus was identified by genomic DNA analysis and phylogenetic tree was constructed using ITS sequences (GenBank) to confirm species. The antibacterial efficacy of Aspergillus sulphureus MME12 ethyl acetate extract was tested against Gram-positive and Gram-negative pathogenic bacteria. DPPH free radical scavenging activity, anticancer and DNA fragmentation against EAC cells, and direct haemolytic activity (100-500 lg/mL) using human erythrocytes were determined. Results and discussion: The ethyl acetate extract of A. sulphureus (Fresen.) Wehmer (Trichocomaceae) demonstrated significant antibacterial potential against Staphylococcus aureus, Bacillus subtilis, Escherichia coli and Salmonella typhi compared to streptomycin. MIC against test pathogens was in the range of 15.6-62.5 lg/mL. The antioxidant results revealed significant RSA from 12.43% to 62.02% (IC 50 ¼ 350.4 lg/ mL, p 0.05). MME12 offered considerable inhibition of EAC proliferation (23% to 84%, IC 50 ¼ 216.7 lg/mL, p 0.05) supported by DNA fragmentation studies. The extract also offered insignificant haemolysis (5.6%) compared to Triton X-100. Conclusions: A single endophytic fungus, A. sulphureus MME12 was isolated and identified using molecular profiling. The above-mentioned findings support the pharmacological application of A. sulphureus MME12 extract and demand for purification of the active principle(s).

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Section: Anticancer and Dna Fragmentation Assaysupporting

confidence: 56%

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Murali

Mahendra

Hema

et al. 2017

Pharmaceutical Biology

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show abstract

“…The endophytic fungus was cultivated on Malt Extract Broth (MEB) (Malt extract, 20 g/l; Dextrose, 20 g/l; Peptone, 6 g/l; pH 5.4 ± 0.2) by inoculating agar blocks of actively growing pure culture (6 mm in diameter) in 250 ml Erlenmeyer flasks containing 100 ml of the medium MEB for 21 days at 28 ± 2°C and 150 rev / min. Fungal mycelia were separated using Whatman filter paper and further centrifuged at 4000 rev / min / min for 5 min (Mai et al, 2013;Astuti et al, 2014).…”

Section: Fermentation and Extractionmentioning

confidence: 99%

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Zerroug¹,

Sadrati²,

Demirel³

et al. 2018

Afr. J. Microbiol. Res.

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In the present study, an endophytic fungus with a considerable antimicrobial activity was isolated from the medicinal plant Mentha Pulegium L. The morphological and molecular identification revealed that this fungus is a Penicillium griseofulvum. A preliminary screening was done to choose the suitable culture medium for a maximum production of the bioactive compounds using the dual-culture agar diffusion assay. The malt extract agar (MEA) and potato dextrose agar (PDA) media were the suitable, and the MEA was selected for the further study. Extraction was done with three solvents, n-Hexane, dichloromethane and ethyl acetate, and the crude extracts were tested against Gram-positive and negative bacteria. A high activity was found with ethyl acetate and dichloromethane crude extracts against all bacteria with a maximal inhibition zones of 45.5 and 41 mm respectively obtained against Escherichia coli. The minimum inhibitory and bactericidal concentrations (MIC, MBC) of ethyl acetate crude extract were evaluated using the broth micro-dilution method. A MIC of 50 µg / ml on Gramnegative bacteria and of 100 µg / ml on Gram-positive bacteria was found. The MBC S ranged from 50 and 200 µg/ml. The time kill study has revealed a bactericidal activity of the Penicillium griseofulvum crude extract. At 24 h and for all concentrations (MIC× 2 and MIC× 4), 100% killing of the bacterial cells was achieved. These results prove that the extract of Penicillium griseofulvum can be a promising source of important bioactive molecules.

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“…So far, a large number of fungal endophytes have been obtained from economically important plants. Some [24]. One hundred and fifty six fungal isolates distributed across 19 taxa were separated from Calotropis procera, and six fungal endophytes were screened to show good antimicrobial activity against human pathogenic microorganisms and one plant pathogenic fungus [25].…”

Section: Discussionmentioning

confidence: 99%

Community of endophytic fungi from the medicinal and edible plant Fagopyrum tataricum and their antimicrobial activity

Zhong¹,

Zou²,

Tang³

et al. 2017

Trop. J. Pharm Res

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Antimicrobial and cytotoxic activities of endophytic fungi isolated from Piper crocatum Ruiz & Pav

Cited by 24 publications

References 22 publications

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Molecular profiling and bioactive potential of an endophytic fungus Aspergillus sulphureus isolated from Sida acuta : a medicinal plant

Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Community of endophytic fungi from the medicinal and edible plant Fagopyrum tataricum and their antimicrobial activity

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