Antibacterial activity of endophytic fungus, Penicillium griseofulvum MPR1 isolated from medicinal plant, Mentha pulegium L.

Abstract

In the present study, an endophytic fungus with a considerable antimicrobial activity was isolated from the medicinal plant Mentha Pulegium L. The morphological and molecular identification revealed that this fungus is a Penicillium griseofulvum. A preliminary screening was done to choose the suitable culture medium for a maximum production of the bioactive compounds using the dual-culture agar diffusion assay. The malt extract agar (MEA) and potato dextrose agar (PDA) media were the suitable, and the MEA was selected for the further study. Extraction was done with three solvents, n-Hexane, dichloromethane and ethyl acetate, and the crude extracts were tested against Gram-positive and negative bacteria. A high activity was found with ethyl acetate and dichloromethane crude extracts against all bacteria with a maximal inhibition zones of 45.5 and 41 mm respectively obtained against Escherichia coli. The minimum inhibitory and bactericidal concentrations (MIC, MBC) of ethyl acetate crude extract were evaluated using the broth micro-dilution method. A MIC of 50 µg / ml on Gramnegative bacteria and of 100 µg / ml on Gram-positive bacteria was found. The MBC S ranged from 50 and 200 µg/ml. The time kill study has revealed a bactericidal activity of the Penicillium griseofulvum crude extract. At 24 h and for all concentrations (MIC× 2 and MIC× 4), 100% killing of the bacterial cells was achieved. These results prove that the extract of Penicillium griseofulvum can be a promising source of important bioactive molecules.

Antibacterial and Antibiofilm Activities of Crude Extract of Lasiodiplodia pseudotheobromae IBRL OS-64 against Foodborne Bacterium, Yersinia enterocolitica

“…Meanwhile, current findings revealed that ethyl acetate extract was the best organic solvent in extracting bioactive compounds with antibacterial activity due to higher inhibitory d a b c activity than other solvents. A similar observation was reported by Zerroug [30] who claimed that the ethyl acetate was the best solvent used for the extraction of bioactive secondary metabolites from an endophytic fungus, Penicillium griseofulvum MPR1. This phenomenon might be due to its efficiency in extracting and isolating secondary metabolites of fungal origin [31].…”

Metabolites of medicine food homology-derived endophytic fungi and their activities

“…An endophytic fungus identified as Penicillium griseofulvum , which shows considerable antimicrobial activity, has been isolated from Mentha pulegium Extracts using ethyl acetate ( 119 ) and dichloromethane ( 120 ) show antimicrobial and anticancer activity ( Amina et al, 2018 ). In one study, Arthrinium sp.…”

“…High activities have been reported for EtOAc ( 119 ) and CHCl 2 ( 120 ) crude extracts of Penicillium griseofulvum MPR1 against E . coli with maximal inhibition zones of 45.5 and 41.0 mm, respectively ( Amina et al, 2018 ). The ethyl acetate extract of Arthrinium sp.…”

Prospecção enzimática e atividade antimicrobiana de espécies de Penicillium isoladas do Bioma Amazônico / Enzyme prospection and antimicrobial activity of Penicillium species isolated from the Amazon Biome

“…Os resultados do presente estudo evidenciam que as espécies de Penicillium oriundas da Amazônia sintetizam amilases e proteases, hidrolases utilizadas em diferentes setores industriais, tais como, alimentício e farmacêutico, além de terem ampla aplicabilidade no beneficiamento do couro, papel e celulose (ALVES et al, 2014). A produção de antimicrobianos por Penicillium é influenciada por diversos parâmetros como tipo de bioprocesso, temperatura e a espécie (BARREIRO; MARTÍN; GARCIA-ESTRADA, 2012;ZERROUG et al, 2018). De acordo com Talukdar et al (2016), a síntese de compostos bioativos por espécies de Penicillium com ação inibitória contra microrganismos também é influenciada pela composição do meio de cultivo, com destaque para as fontes de carbono, nitrogênio e minerais.…”