Antimicrobial and extracellular oxidative enzyme activities of endophytic fungi isolated from alfalfa (Medicago sativa) assisted by metabolic profiling

Attia, Eman Zekry; Farouk, Hala Montaser; Abdelmohsen, Usama Ramadan; El-Katatny, Mo'men H.

doi:10.1016/j.sajb.2019.12.003

Cited by 24 publications

(19 citation statements)

References 42 publications

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“…Endophytic Aspergillus have been described from the arctic tundra to the tropics [ 1 ]. Specifically, A. ochraceus has been isolated from the fern Selaginella stauntoniana [ 25 ]; a wide range of plants including Euphorbia geniculata [ 26 ] Polygonatum cyrtonema [ 27 ], alfalfa ( Medicago sativa ) [ 28 ], bamboo [ 29 ], the cactus Cereus jamacaru [ 30 ], Bauhinia forficata [ 31 ]; orchids ( Bulbophyllum neilgherrense and Vanda testacea ) [ 32 ]; and Catha edulis [ 33 ]. A. ochraceus has also been isolated as an endosymbiont from marine organisms such as the coral Dichotella gemmacea [ 34 ] and the marine brown alga Sargassum kjellmanianum [ 35 , 36 ].…”

Section: Discussionmentioning

confidence: 99%

Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2

Morales-Sánchez

Díaz

Trujillo

et al. 2021

JoF

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In the current study, an ethyl acetate extract from the endophytic fungus Aspergillus sp. SPH2 isolated from the stem parts of the endemic plant Bethencourtia palmensis was screened for its biocontrol properties against plant pathogens (Fusarium moniliforme, Alternaria alternata, and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi), plant parasites (Meloidogyne javanica), and ticks (Hyalomma lusitanicum). SPH2 gave extracts with strong fungicidal and ixodicidal effects at different fermentation times. The bioguided isolation of these extracts gave compounds 1–3. Mellein (1) showed strong ixodicidal effects and was also fungicidal. This is the first report on the ixodicidal effects of 1. Neoaspergillic acid (2) showed potent antifungal effects. Compound 2 appeared during the exponential phase of the fungal growth while neohydroxyaspergillic acid (3) appeared during the stationary phase, suggesting that 2 is the biosynthetic precursor of 3. The mycotoxin ochratoxin A was not detected under the fermentation conditions used in this work. Therefore, SPH2 could be a potential biotechnological tool for the production of ixodicidal extracts rich in mellein.

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Section: Discussionmentioning

confidence: 99%

Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2

Morales-Sánchez

Díaz

Trujillo

et al. 2021

JoF

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“…Over 120 natural bioactive compounds are produced by Aspergillus species (Lee et al 2013). A. ochraceus has shown good antimicrobial properties in a study by Attia et al (2019). LC-MS studies have shown that it produces the previously reported antimicrobial compounds versicolin, terreic acid, fumigatin, aspyrone, 4-hydroxymellin and terremutin.…”

Section: Molecular Characterisation and Antimicrobial Propertiesmentioning

confidence: 99%

Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius

et al. 2021

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Purpose of the study: Marine fungi of Mauritius have been poorly studied. There are numerous reports on the bioactive secondary metabolites that are produced by fungi around the world. Yet, research on the molecular characterisation and the pharmaceutical potential of marine fungi in Mauritius is rather scanty. Method: The samples, which consisted of three sponges Haliclona sp., Iotrochota sp. and Biemna sp. and two brown algae Turbinaria conoides and Sargassum portierianum, were collected in the North of Mauritius during winter. No sporulating structures were observed from the fungal cultures making morphological analysis impossible. The molecular characterisation of the selected isolates was carried out by the amplification of the ITS regions and phylogenetic analysis. The antimicrobial properties were then determined using the disc diffusion and the minimum inhibitory concentration (MIC) assay. Results: Genus level identification was made from molecular data and for some isolates, specieslevel identification was even possible. Twelve fungi that showed the best antimicrobial properties were identified as Peniophora sp., Aspergillus cristatus, Acremonium sp., Cordyceps memorabilis, Aspergillus ochraceus, Biscogniauxia sp., Aspergillus keratitidis, Exserohilum rostratum, Chromocleista sp., Nigrospora oryzae, Aspergillus flavipes and Mycosphaerella. The lowest MIC result of 0.0098 mg/ mL was obtained with Chromocleista sp. mycelium extract against Staphylococcus aureus. The MIC of the mycelium extracts was lower than the broth extracts for most isolates indicating that the antimicrobial compounds are not secreted. Conclusion:Marine fungi from the Mauritian waters have immense potential in the search for natural products against antibiotic-resistant bacteria.

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“…Endophytic Aspergillus have been described from the arctic tundra to the tropics [1]. Specifically, A. ochraceus has been isolated from the fern Selaginella stauntoniana [30]; and a wide range of plants including Euphorbia geniculata [31] Polygonatum cyrtonema [32], alfalfa (Medicago sativa) [33], bamboo [34], the cactus Cereus jamacaru [35], Bauhinia forficata [36]; orchids (Bulbophyllum neilgherrense and Vanda testacea) [37], and Catha edulis [38]. A. ochraceus has also been isolated as an endosymbiont from marine organisms such as the coral Dichotella gemmacea [39] and the marine brown alga Sargassum kjellmanianum [40,41].…”

Section: Discussionmentioning

confidence: 99%

Bioactive Metabolites from the Endophytic Fungus <em>Aspergillus sp. </em>SPH2

Morales-Sánchez¹,

Díaz²,

Trujillo³

et al. 2020

Preprint

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In the current study, an ethyl acetate extract from the endophytic fungus Aspergillus ochraceus SPH2 isolated from the stem parts of the endemic plant Bethencourtia palmensis was screened for its biocontrol properties against plant pathogens (Fusarium moniliforme, Alternaria alternata and Botrytis cinerea), insect pests (Spodoptera littoralis, Myzus persicae, Rhopalosiphum padi), plant parasites (Meloidogyne javanica) and ticks (Hyalomma lusitanicum). SPH2 gave extracts with strong fungicidal and ixodicidal effects at different fermentation times. The bioguided isolation of these extracts gave compounds 1-3. Mellein (1) showed strong ixodicidal effects and was also fungicidal. This is the first report on the ixodicidal effects of 1. Neoaspergillic acid (2) showed potent antifungal effects. Compound 2 appeared during the exponential phase of the fungal growth while neohydroxyaspergillic acid (3) appeared during the stationary phase, suggesting that 2 is the biosynthetic precursor of 3. Additional molecular ions compatible with pyrazynes that were detected during the exponential phase included flavacol, and aspergilliamide while ochramide A was mostly detected during the stationary phase of the fermentation. Moreover, polyketids were also detected during the sationaty phase of the fermentation curve (dihydroaspyrone, aspyrone, asperlactone) and the alkaloid circumdatin H. Ochratoxin A was not detected. Therefore, SPH2 could be a potential biotechnological tool for the production of ixodicidal mellein.

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Antimicrobial and extracellular oxidative enzyme activities of endophytic fungi isolated from alfalfa (Medicago sativa) assisted by metabolic profiling

Cited by 24 publications

References 42 publications

Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2

Bioactive Metabolites from the Endophytic Fungus Aspergillus sp. SPH2

Antimicrobial properties of marine fungi from sponges and brown algae of Mauritius

Bioactive Metabolites from the Endophytic Fungus <em>Aspergillus sp. </em>SPH2

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