Antimicrobial and nematicidal screening of anamorphic fungi isolated from plant debris of tropical areas in Mexico

Reyes-Estébanez, Manuela; Herrera-Parra, Elizabeth; Cristóbal‐Alejo, Jairo; Heredia, Gabriela; Canto-Canché, Blondy; Medina-Baizabal, Irma L.; Gamboa‐Angulo, Marcela

doi:10.5897/ajmr11.121

Cited by 3 publications

(12 citation statements)

References 36 publications

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“…As results, the Selenosporella sp. MR26 strain has shown to have good effectivity against M. incognita (Reyes- Estebanez et al, 2011). However, in this study the nematotoxic effect detected was low (2 %), when strains were grown in fermented rice.…”

Section: In Vitro Toxicity Of Tropical Mexican Micromycetes On Infective Juveniles Of Meloidogyne Incognitacontrasting

confidence: 58%

“…The toxicity of the filtrates and methanol extract obtained from liquid culture medium from Selenosporella sp. MR26 was higher as compare to the one shown by extracts derived from solid medium (Reyes-Estebanez et al, 2011).…”

Section: Resultados Y Discusiónmentioning

confidence: 58%

“…Culture conditions and metabolites fungal extraction. All strains were cultured in potato dextrose agar (PDA) media at room temperature (25 ± 2 °C, 16/8 hours light/darkness) for one week, to obtain a suspension of hyphal fragments-spore suspension (Reyes-Estebanez et al, 2011). One mL of the suspension was transferred to Czapek-Dox liquid medium (200 mL) contained in Roux bottles, with three replicates per isolate.…”

Section: Methodsmentioning

confidence: 99%

“…TA34, and four unidentified strains: TA13, 2TA6, 2TA7, and 2XA7). These strains were grown in Czapeck Dox, a defined liquid medium (Gamboa-Angulo et al, 2012;Reyes-Estebanez et al, 2011).…”

Section: Materiales Y Métodosmentioning

confidence: 99%

“…Furthermore, it was interesting to detect that Selenosporella sp. MR26 produces non polar nematotoxic metabolites when grown on solid fermented rice media (EC 50 values of 0.91 mg mL -1 ) and polar in Czapeck-Dox liquid medium (Reyes-Estebanez et al, 2011). Actually, the isolation and identification of the metabolites of Selenosporella sp.…”

Section: Conclusionesmentioning

confidence: 99%

See 4 more Smart Citations

Toxicidad in vitro de Micromicetos del Trópico Mexicano en Juveniles Infectivos de Meloidogyne incognita

Angulo

Escobar

Herrera-Parra

et al. 2015

RMF

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Filtrados de cultivos y extractos de micelio (acetato de etilo y metanol) de nueve cepas fúngicas Mexicanas tropicales se evaluaron en condiciones in vitro contra juveniles de segundo estadio (J2)de Meloidogyne incognita. Los micromicetos Acremonium kiliense TA31, Aspergillus sp. 2XA5, Gliocladium sp. MR41, Selenosporella sp. MR26, Stagonospora sp. TA34 y cuatro cepas no identificadas (TA13, 2TA6, 2TA7 y 2XA7) se cultivaron en medio Czapeck-Dox durante 14 días y la masa micelial se separó por filtración para la extracción de metabolitos. Aspergillus sp. 2XA5 y Selenosporella sp. MR26 mostraron la mayor actividad nematóxica, tanto en filtrados de cultivo (100% de mortalidad) como en extractos metanólicos (> 90% de mortalidad). La CE50 más baja (0.08 mg mL-1) se obtuvo con el extracto de metanol de la cepa 2XA7 no identificada. Los resultados obtenidos indican que la micobiota tropical tiene potencial como agentes de control biológico de nematodos fitopatógenos.

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Section: In Vitro Toxicity Of Tropical Mexican Micromycetes On Infective Juveniles Of Meloidogyne Incognitacontrasting

confidence: 58%

Section: Resultados Y Discusiónmentioning

confidence: 58%

Section: Methodsmentioning

confidence: 99%

“…TA34, and four unidentified strains: TA13, 2TA6, 2TA7, and 2XA7). These strains were grown in Czapeck Dox, a defined liquid medium (Gamboa-Angulo et al, 2012;Reyes-Estebanez et al, 2011).…”

Section: Materiales Y Métodosmentioning

confidence: 99%

Section: Conclusionesmentioning

confidence: 99%

See 3 more Smart Citations

Toxicidad in vitro de Micromicetos del Trópico Mexicano en Juveniles Infectivos de Meloidogyne incognita

Angulo

Escobar

Herrera-Parra

et al. 2015

RMF

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Identification of Insect-Deterrent Metabolites from Acremonium masseei strain CICY026, a Saprophytic Fungus from a Sinkhole in Yucatán

et al. 2019

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Micromycetes from unexplored sources represent an opportunity to discover novel natural products to control insect pests. With this aim, a strain of Acremonium masseei CICY026 isolated from a tropical sinkhole was identified, cultured on fermented rice, and its ethyl acetate extract (EAE) was evaluated against three serious phytophagous insects (Bemisia tabaci, Myzus persicae, and Rhopalosiphum padi). DNA from A. masseei CICY026 was used to confirm its identity. EAE caused settling inhibition (SI) of M. persicae and R. padi (67.5% and 75.3%, respectively). Bioassay-guided fractionation of the active EAE led to the isolation of a novel metabolite, named hexahydroacremonintriol (1), and of acremonin A glucoside (2). The structures of 1 and 2 were determined using IR, one- and two-dimensional NMR, HRMS, and confirmed by theoretical data. The aphid M. persicae was noticeably sensitive to 1 and 2 (SI: 55.6% and 67.2%, respectively), whereas R. padi was only slightly affected by 1 (SI: 59%). This new knowledge about mycobiota from these special sinkhole ecosystems will inform the development of new biorational pesticides.

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Actividad Antimicrobiana De Extractos Obtenidos De La Interacción Dual Entre Micromicetos Saprobios

Medina-Baizabal

Abarca

Gamboa‐Angulo

2022

Trop Subtrop Agroecosyst

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Background: Saprophytic micromycetes are recognized for their extraordinary ability to biosynthesize various metabolites with biological properties that can be modified when interacting with other organisms. Objective: To evaluate the antibacerial and antifungal activity of extracts obtained from dual interactions of Acremonium masseei CICY029 (Am), Beltraniella portoricensis MR42 (Bp), Cylindrium elongatum MR45 (Ce) y Stachybotrys sp. MR33 (Ssp) and to obtain the chemical profile of the most active interaction. Methodology: The ethyl acetate extracts of the four strains and their six dual interactions were evaluated against ten pathogens by the microdilution method to determine their antifungal and antibacterial capacity. The most active extracts were partitioned, and the minimum inhibitory concentration (MIC) o their fractions were determined. The chemical profile of the most active interaction was analyzed by gas chromatography coupled to mass spectrometry. Results: The highest mycelial growth inhibitory activity (MCI = 100%) was obtained from the fungal extracts of the single culture of Stachybotrys sp., and the dual Ce-Ssp interaction against Colletotrichum gloeosporioides, Mycosphaerella fijiensis at the concentration of 2000 µg/mL, as well as against Candida albicans and Xanthomonas campestris at 400 µg/mL. The acetonitrile fraction of Stachybotrys sp. showed high effectiveness against Alternaria tagetica, C. gloeosporioides, M. fijiensis and X. campestris (MIC ≤ 250 -1000 µg/mL). A mixture of fatty acids and three unidentified components not observed in the single cultures were detected by GC-MS in the Ce-Ssp extract. Implications: Biological interactions between two saprophytic fungi are a viable alternative for inducing the production of new metabolites with the potential to control pathogenic bacteria and fungi. Conclusions: The antifungal and antibacterial potential of saprophytic micromycetes can be improved in their dual interaction, particularly between C. elongatum MR45 and Stachybotrys sp. MR33, is a promising option for further investigation as potential natural antimicrobials.

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Antimicrobial and nematicidal screening of anamorphic fungi isolated from plant debris of tropical areas in Mexico

Cited by 3 publications

References 36 publications

Toxicidad in vitro de Micromicetos del Trópico Mexicano en Juveniles Infectivos de Meloidogyne incognita

Toxicidad in vitro de Micromicetos del Trópico Mexicano en Juveniles Infectivos de Meloidogyne incognita

Identification of Insect-Deterrent Metabolites from Acremonium masseei strain CICY026, a Saprophytic Fungus from a Sinkhole in Yucatán

Actividad Antimicrobiana De Extractos Obtenidos De La Interacción Dual Entre Micromicetos Saprobios

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