Antimicrobial and osteogenic properties of a hydrophilic-modified nanoscale hydroxyapatite coating on titanium

Murakami, Asuka; Arimoto, Takafumi; Suzuki, Daisuke; Iwai-Yoshida, Misato; Otsuka, Fukunaga; Shibata, Yo; Igarashi, Takeshi; Kamijo, Ryutaro; Miyazaki, Takashi

doi:10.1016/j.nano.2011.07.001

Cited by 40 publications

(24 citation statements)

References 41 publications

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“…Contact angle measurement can also provide supporting evidence that the Ti surface has been successfully modified (Table 1). 33,34 The pristine Ti substrate is relatively hydrophobic (with a contact angle of 61°-1.2°), but after CaP coating and alendronate loading, the contact angle decreases due to the hydrophilicity of CaP and alendronate. 33,34 The amount of alendronate bound to the Ti-CaP substrates is also shown in Table 1.…”

Section: Surface Characterization and Alendronate Release Testmentioning

confidence: 99%

“…33,34 The pristine Ti substrate is relatively hydrophobic (with a contact angle of 61°-1.2°), but after CaP coating and alendronate loading, the contact angle decreases due to the hydrophilicity of CaP and alendronate. 33,34 The amount of alendronate bound to the Ti-CaP substrates is also shown in Table 1. When the alendronate concentration in the loading solution increased from 0.2 to 1 mg/mL, the surface density of loaded alendronate linearly increased from 0.019 -0.01 mg/cm 2 (Ti-CaP-Alen0.2) to 0.11 -0.02 mg/cm 2 (Ti-CaP-Alen1).…”

Section: Surface Characterization and Alendronate Release Testmentioning

confidence: 99%

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AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

Neoh

Shi

et al. 2013

Tissue Engineering Part A

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Fibrous encapsulation can impair implant osseointegration and cause implant failure but currently there are limited strategies to address this problem. Since bisphosphonates (BPs), a class of drugs widely used to treat bone diseases, was recently found to induce fibroblast apoptosis, we hypothesize that by loading BPs on titanium (Ti) implant surface, fibrous encapsulation may be inhibited with simultaneous enhancement of implant osseointegration. This strategy of local administration can also be expected to minimize the adverse side effects of BPs, which are associated with intravenous injections. To verify this hypothesis, alendronate was loaded on Ti surface via a hydroxyapatite (CaP) coating, and the effects of the loaded alendronate on fibroblast proliferation and apoptosis, and osteoblast proliferation, alkaline phosphatase (ALP) activity, and apoptosis were investigated in vitro. With a surface density of loaded alendronate 0.046 mg/cm 2 or higher, fibroblast proliferation was suppressed due to increased apoptosis, while osteoblast proliferation and ALP activity increased with minimal apoptosis. In a coculture of fibroblasts and osteoblasts in a 1:1 ratio, *60% of the cells on these alendronate-loaded substrates were osteoblasts 1 day after cell seeding. The percentage of osteoblasts increased to about 75% 4 days after cell seeding. These results suggest that fibroblasts and osteoblasts respond differently toward the alendronate-modified substrates, and this phenomenon can potentially be capitalized to reduce fibrous encapsulation.

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Section: Surface Characterization and Alendronate Release Testmentioning

confidence: 99%

Section: Surface Characterization and Alendronate Release Testmentioning

confidence: 99%

AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

Neoh

Shi

et al. 2013

Tissue Engineering Part A

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“…Hydroxyapatite (HA) is a widely used biomaterial in orthopaedic implant technology as a bone graft substitute [1,2,3,4,5] and as a coating for titanium implants [6,7,8,9]. Traditional bone implant technologies have focused on the mechanical properties of implants to optimise their performance [10].…”

Section: Introductionmentioning

confidence: 99%

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

Neary

Blom

Shiel

et al. 2018

J Mater Sci: Mater Med

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There is an incentive to functionalise hydroxyapatite (HA) for orthopaedic implant use with bioactive agents to encourage superior integration of the implants into host bone. One such agent is (3S) 1-fluoro-3-hydroxy-4-(oleoyloxy) butyl-1-phosphonate (FHBP), a phosphatase-resistant lysophosphatidic acid (LPA) analogue. We investigated the effect of an FHBP-HA coating on the maturation of human (MG63) osteoblast-like cells. Optimal coating conditions were identified and cell maturation on modified and unmodified, control HA surfaces was assessed. Stress tests were performed to evaluate coating survivorship after exposure to mechanical and thermal insults that are routinely encountered in the clinical environment. MG63 maturation was found to be three times greater on FHBP-modified HA compared to controls (p < 0.0001). There was no significant loss of coating bioactivity after autoclaving (P = 0.9813) although functionality declined by 67% after mechanical cleaning and reuse (p < 0.0001). The bioactivity of modified disks was significantly greater than that of controls following storage for up to six months (p < 0.001). Herein we demonstrate that HA can be functionalised with FHBP in a facile, scalable manner and that this novel surface has the capacity to enhance osteoblast maturation. Improving the biological performance of HA in a bone regenerative setting could be realised through the simple conjugation of bioactive LPA species in the future. Depicted is a stylised summary of hydroxyapatite (HA) surface modification using an analogue of lysophosphatidic acid, FHBP. a HA surfaces are simply steeped in an aqueous solution of 2 μM FHBP. b The polar head group of some FHBP molecules react with available hydroxyl residues at the mineral surfaces forming robust HA-O-P bonds leaving acyl chain extensions perpendicular to the HA surface. These fatty acyl chains provide points of integration for other FHBP molecules to facilitate their self-assembly. This final surface finish enhanced the human osteoblast maturation response to calcitriol, the active vitamin D3 metabolite.

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“…Due to these facts, HAp is highly used as bone substitutes and as coatings on metallic prostheses. Usually, HAp coatings have an important contribution to the bone formation necessary for implant osseointegration and generally provide an early fixation between the bones and implant surfaces . Despite the fact that HAp coatings are widely used, there are concerns regarding the relatively low survival rate of HAp‐coated metallic implants, which was attributed to the integrity of bonding between the HAp layer and the implant surface.…”

Section: Introductionmentioning

confidence: 99%

The Effect of the Ionizing Radiation on Hydroxyapatite–Polydimethylsiloxane Layers

Groza

Iconaru

Jiga

et al. 2019

Polymer Engineering & Sci

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The bio hydroxyapatite (HAp) was used from a long time in different medical and environmental applications. The HAp layers with a uniform surface were used for various medical applications such as orthopedic and dental metal implants. In this work, we reported on the influence of X‐ray radiation on the structural and morphological properties of composite layers based on HAp and polydimethylsiloxane (PDMS) deposited on titanium substrates. The HAp:PDMS layers were investigated by different complementary methods such as scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FTIR), and glow discharge optical emission spectrometry (GDOES). FTIR spectral analysis showed that the molecular structure of the coatings was not changed after their irradiation even though, the depth profile analysis performed by GDOES indicated a depletion of Ca and P elements from the HAp:PDMS irradiated samples. By SEM, we showed that the morphological features of the coatings were also changed, as the irradiated layers are delaminated. The biological assays confirmed that the antibacterial activity of HAp:PDMS composite layers increased after irradiation. The results obtained in this study highlighted that the biological properties of HAp:PDMS layers could be influenced by irradiation. POLYM. ENG. SCI., 59:2406–2412, 2019. © 2019 Society of Plastics Engineers

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Antimicrobial and osteogenic properties of a hydrophilic-modified nanoscale hydroxyapatite coating on titanium

Cited by 40 publications

References 41 publications

AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

AnIn VitroAssessment of Fibroblast and Osteoblast Response to Alendronate-Modified Titanium and the Potential for Decreasing Fibrous Encapsulation

Development and biological evaluation of fluorophosphonate-modified hydroxyapatite for orthopaedic applications

The Effect of the Ionizing Radiation on Hydroxyapatite–Polydimethylsiloxane Layers

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