Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Galium mexicanum

Bolívar, Paulina; Cruz-Paredes, Carla; Hernández, Lucina; Juárez, Zaida Nelly; Sánchez‐Arreola, Eugenio; Av‐Gay, Yossef; Bach, Horacio

doi:10.1016/j.jep.2011.04.069

Cited by 40 publications

(29 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The fungal strain of A. parasitcus and P. expansum were cultured in Potato Dextrose Broth (PDB) and antifungal activity was assessed with the method of Bolivar et al (2011) with some modifications. Peptides previously isolated were dissolved in sterile water with concentrations ranging from 10 to 15,000 ppm.…”

Section: Antifungal Activity In Liquid Mediummentioning

confidence: 99%

In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

Luz¹,

Saladino²,

Luciano³

et al. 2017

LWT - Food Science and Technology

View full text Add to dashboard Cite

a b s t r a c tFood spoilage caused by mycotoxigenic moulds represents an important problem in food security. The antimicrobial peptides are compounds of natural origin constituted by a variable number (5e100) of amino acids held together through peptide bonds. In this work, the cell free supernatants (CFSs) containing peptides obtained from four strains of LAB were lyophilized, filtered and tested to determine the antifungal activity against Aspergillus Parasiticus and Penicillium expansum. CFS obtained by Lactobacillus plantarum showed the highest inhibition activity. CFS was fractionated by size exclusion chromatography and injected into the liquid chromatography coupled to diode array detector. One of the recollected fractions resulted interesting for the presence of three peaks that were purified by the technique of the LC-DAD using a semi preparative C18 column. Finally, the antifungal activity of the purified peptides was studied against A. Parasiticus and P. expansum in liquid medium. The MALDI-TOF/TOF mass spectrometry was used for the peptides identification. The three purified peptides presented an amino acidic sequence identified by a bioinformatics program of SGADTTFLTK, LVGKKVQTF, and GTLIGQDYK. The first peptide purified reduced 58% and 73% the growth of P. expansum and A. parasiticus, respectively, in liquid medium after 48 h incubation.

show abstract

Section: Antifungal Activity In Liquid Mediummentioning

confidence: 99%

In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

Luz¹,

Saladino²,

Luciano³

et al. 2017

LWT - Food Science and Technology

View full text Add to dashboard Cite

show abstract

“…Anti-inflammatory activity and antibacterial effects have been described against S. aureus (MIC 67μg/mL), and Bacillus subtilis (MIC 67μg/mL) of hexane and chloroform G. mexicanum extracts (Bolívar et al, 2011). The current study is the first to report that methanolic extract of G. mexicanum showed antitopoisomerase type I activity.…”

Section: Discussionmentioning

confidence: 49%

Evaluation of Anticancer Potential of Eight Vegetal Species From the State of Oaxaca

León¹,

Herrera-Martínez²,

Ramirez-Mares³

et al. 2017

Afr J Tradit Complement Altern Med

View full text Add to dashboard Cite

Background: Eight plant species from Oaxaca, some of them used in traditional medicine, were subjected to screening of several biological activities to provide data regarding their anticancer potential, although no scientific information is available about their pharmacological effects. Materials and methods: Methanol extracts from stems or roots of the eight plants were tested for antioxidant activity by the DPPH· method. Antimicrobial activity was determined using the agar diffusion method and the minimal inhibitory concentration (MIC) was obtained by broth dilution method. Antitopoisomerase activity was assessed using mutant strains of Saccharomyces cerevisiae JN362a, JN394, JN394t -1 , JN394t 2-4 and JN394t 2-5 . The mutagenic activity was evaluated using the Ames test (Salmonella typhimurium TA1535). Results:No extract showed significant antioxidant activity. The best antimicrobial activity was observed for Salpianthus arenarius (MIC 56.25 μg/mL) and Lantana achyranthifolia (MIC 78.12 μg/mL) against Staphylococcus aureus. Extracts of Acalypha cuspidata, Alloispermum integrifolium and L. achyranthifolia stems showed antitopoisomerase II activity with JN394 t-1 growth of -30.88±0.0%, -38.11±4.95%, and -70.97±12.02% respectively. Galium mexicanum stem extract showed antitopoisomerase I activity with growth of 35.31±6.36% on the same mutant strain. All plant extracts were non-mutagenic. Fractionation of A. cuspidata extract led to identification of two subfractions with antitopoisomerase I and II activity at 154μg/mL (Positive controls 50 and 100μg/mL). Conclusion: Methanol extracts of A. cuspidata, A. integrifolium, G. mexicanum, and L. achyranthifolia stems showed antitopoisomerase and non-mutagenic activities, and consequently could be promising as a source of anticancer drugs.

show abstract

“…Plant extracts included in the CRB have been reported in the literature to possess antioxidative, antiinflammatory and tissue regenerating properties. Species from the Galium genus have shown efficacy in reduction of oxidative stress in dermal fibroblasts (Chaher et al 2016) and proved to be potent antiiflammatory agents (Bolivar et al 2011). Vaccinium myrtillus extracts are reported to exert protective activity in UVA-irradiated keratinocytes (Calò, Marabini 2014).…”

Section: Discussionmentioning

confidence: 99%

Chemical characterization and in vitro evaluation of birch sap and a complex of plant extracts for potential use in cosmetic anti-ageing products

2017

EEB

View full text Add to dashboard Cite

Cosmetic manufacturers are continuously searching for new active ingredients to satisfy consumer needs. This leads to growing demand of botanical extracts with characterized chemical composition and proven efficacy. The aim of the study was to characterize the chemical composition of birch sap and a complex of seven plant extracts, with brand name Cell Repair Bio-Complex (CRB), and to evaluate their effects on skin cells. HPLC analysis was used to identify dominating compounds. Effect on proliferation of dermal fibroblasts and keratinocytes was assessed using the IncuCyte ZOOM system. Results show that flavonoid and phenolic acid rich CRB possesses antiradical activity and stimulates dermal fibroblast proliferation at low concentration. Birch sap stimulates both dermal fibroblasts and keratinocytes at high concentrations. Stimulatory effect of keratinocytes was observed also for the combination of birch sap and CRB. The findings indicate the potential of CRB to be used as an active ingredient, whereas birch sap used at high concentrations could substitute water base and add additional efficacy.Key words: birch sap, dermal fibroblasts, epidermal keratinocytes, plant extracts, skin antiageing. Abbreviations: ARA, antiradical activity; CRB, Cell Repair Bio-Complex (a brand name for a combination of seven plant extracts for cosmetic use); DPPH, 2.2-diphenyl-1-picrylhydrazyl radical; ROS, reactive oxygen species.

show abstract

Antimicrobial, anti-inflammatory, antiparasitic, and cytotoxic activities of Galium mexicanum

Cited by 40 publications

References 19 publications

In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

In vitro antifungal activity of bioactive peptides produced by Lactobacillus plantarum against Aspergillus parasiticus and Penicillium expansum

Evaluation of Anticancer Potential of Eight Vegetal Species From the State of Oaxaca

Chemical characterization and in vitro evaluation of birch sap and a complex of plant extracts for potential use in cosmetic anti-ageing products

Contact Info

Product

Resources

About