Antimicrobial components of some cruciferae plants (Diplotaxis harra Forsk. and Erucaria microcarpa Boiss.)

Hashem, F. A.; Saleh, Malaka A.

doi:10.1002/(sici)1099-1573(199906)13:4<329::aid-ptr458>3.3.co;2-l

Cited by 13 publications

(14 citation statements)

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“…The thioglucosidase hydrolysed fraction was prepared according to (William et al ., ; Hashem and Wahba, ) as follows: the frozen broccoli florets were sliced (500.0 g), homogenized in a mixer with 2500 mL water, after the addition of previously prepared thioglucosidase enzyme (Hashem and Saleh, ) and then left overnight. The homogenate was centrifuged at 8000 rpm for 5 min.…”

Section: Methodsmentioning

confidence: 99%

Myrosinase Hydrolysates of Brassica oleraceae L. Var. italica Reduce the Risk of Colon Cancer

Hashem

Motawea

El-Shabrawy

et al. 2011

Phytotherapy Research

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By means of liquid chromatography-electrospray ionization (LC-ESI) mass spectrometry two glucosinolates, glucoiberin and 3-hydroxy,4(α-L-rhamnopyranosyloxy) benzyl glucosinolate, were identified in the aqueous extract of Brassica oleraceae L var. italica. Further, two compounds were isolated after enzymatic hydrolysis of the aqueous extract by myrosinase, one of them was identified as 4-vinyl-3-pyrazolidinone. The second compound (sulphoraphane) 1-isothiocyanate-4-methyl-sulphinyl butane, converted to the most stable form of thiourea (sulphoraphane thiourea). The crude extract (80% alcohol extract) of broccoli florets was examined for cytotoxic activity against different human cancer cell lines, it showed good inhibition of colon cancer (IC(50) 3.88 µg/mL). On the other hand each of the successive extracts (petroleum ether, chloroform, ethyl acetate and ethanol) showed no significant cytotoxic activity. When myrosinase hydrolysate was tested for cytotoxic activity on the colon cancer cell line it showed very high activity - 95% lethality up to 0.78 µg/mL.

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Section: Methodsmentioning

confidence: 99%

Myrosinase Hydrolysates of Brassica oleraceae L. Var. italica Reduce the Risk of Colon Cancer

Hashem

Motawea

El-Shabrawy

et al. 2011

Phytotherapy Research

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“…In 1995, Brabban and Edwards reported that The antibacterial properties of isothiocyanates only the hydrolysis products of the glucosinolate sinigrin displayed an effect upon the growth of micro-organisms (Brabban & Edwards, 1995). Several years later, the volatile ITC components of two Brassicaceae plant extracts were identified for their activity against yeasts, bacteria and fungi (Hashem & Saleh, 1999). There is now ample evidence for the antimicrobial properties of ITCs (Aires et al, 2009a, b), but most reports of the suppression of bacterial growth by ITCs are still limited to MIC determination.…”

Section: Mic Against Various Pathogensmentioning

confidence: 99%

The antibacterial properties of isothiocyanates

2015

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Isothiocyanates (ITCs) are natural plant products generated by the enzymic hydrolysis of glucosinolates found in Brassicaceae vegetables. These natural sulfur compounds and their dithiocarbamate conjugates have been previously evaluated for their anti-cancerous properties. Their antimicrobial properties have been previously studied as well, mainly for food preservation and plant pathogen control. Recently, several revelations concerning the mode of action of ITCs in prokaryotes have emerged. This review addresses these new studies and proposes a model to summarize the current knowledge and hypotheses for the antibacterial effect of ITCs and whether they may provide the basis for the design of novel antibiotics.

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“…No entanto, os extratos avaliados neste estudo mostraram-se ativos contra cepas Gram positivas e Gram negativas. Os ácidos graxos presentes, principalmente, nos extratos lipofílicos podem ser os responsáveis pela atividade antimicrobiana encontrada nas plantas desse estudo (Hashem;Saleh, 1999;Nazif, 2002). Foram identifi cados oito ácidos graxos diferentes nos extratos de S. oleracea e M. vinifera, como pode ser visto na Tabela 3.…”

Section: Discussionunclassified

Atividade antimicrobiana dos frutos de Syagrus oleracea e Mauritia vinifera

Silveira¹,

Pessanha²,

Lourenço³

et al. 2005

Rev. bras. farmacogn.

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RESUMO:As espécies pertencentes à família Palmae são muito interessantes do ponto de vista químico e farmacológico. Neste trabalho, foram estudados os frutos de duas espécies da família Palmae, Syagrus oleracea e Mauritia vinifera. Essas palmeiras foram escolhidas por serem espécies brasileiras, abundantes em nosso país, utilizadas popularmente no tratamento de algumas doenças e ainda pouco estudadas. Foram realizados ensaios farmacológicos para avaliação da atividade antimicrobiana dos extratos dos frutos das duas espécies em estudo. ABSTRACT: "Antimicrobial activity of Syagrus oleracea and Mauritia vinifera fruits". Palmae species are very interesting by the chemical and pharmacological points of view. Two species belonging to this family were chosen to initiate the chemical and pharmacological approach of their fruits: Syagrus oleracea (Martius) Beccari and Mauritia vinifera Martius, known in Brazil as Guariroba and Buriti, respectively. Those palm species can be found in several regions of Brazil, especially at the northeast and southeast of the country. They have been used in folk medicine to treat some diseases, however no toxicological and pharmacological studies have been done so far. For the two studied fruits, the antimicrobial activity tests were carried out by broth microdilution methodology. The objective of this work was to contribute for the pharmacological study of palm species, evaluating the antimicrobial activity of the extracts obtained from the fruits of S. oleracea and M. vinifera. The assays evaluated ethanol extracts of the epicarp/mesocarp of S. oleracea and epicarp/mesocarp of M. vinifera; hexane extract of the endosperm of S. oleracea; hexane and ethyl acetate fractions of the epicarp/mesocarp of S. oleracea, epicarp/mesocarp of M. vinifera and mesocarp/endocarp of M. vinifera. The lipophilic extracts of S. oleracea obtained the best results for the species. For M. vinifera, the lipophilic partitions have shown a high inhibitory percentage for S. aureus. Keywords:Syagrus oleracea, Mauritia vinifera, Palmae, antimicrobial activity. INTRODUÇÃOAs espécies pertencentes à família Palmae, comumente chamadas de palmeiras, são muito interessantes do ponto de vista químico e farmacológico. As palmeiras estão amplamente distribuídas nas zonas temperadas de todo o mundo, principalmente em regiões onde o índice pluviométrico é alto (Cruz, 1965;Zofemler, 1994). Do ponto de vista químico, as plantas dessa família são geralmente não cianogênicas. Os alcalóides (ocasionalmente pirimidínicos) e proantocianidinas podem estar presentes ou não. Os fl avonóides são raros, mas quando presentes são derivados do kaempferol, quercetina, tricina e luteolina. Saponinas e sapogeninas estão ocasionalmente presentes. Éteres metílicos de triterpenos já foram isolados dos frutos de algumas

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Antimicrobial components of some cruciferae plants (Diplotaxis harra Forsk. and Erucaria microcarpa Boiss.)

Cited by 13 publications

References 0 publications

Myrosinase Hydrolysates of Brassica oleraceae L. Var. italica Reduce the Risk of Colon Cancer

Myrosinase Hydrolysates of Brassica oleraceae L. Var. italica Reduce the Risk of Colon Cancer

The antibacterial properties of isothiocyanates

Atividade antimicrobiana dos frutos de Syagrus oleracea e Mauritia vinifera

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