Antimicrobial Compound from Endophytic Pseudomonas azotoformans UICC B-91 of Neesia altissima (Malvaceae)

Pratiwi, Rina Hidayati; Oktarina, Eva; Mangunwardoyo, Wibowo; Hidayat, Iman; Saepudin, Endang

doi:10.5530/pj.2022.14.23

Cited by 6 publications

(6 citation statements)

References 30 publications

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“…KB has united a variety of interactions, such as those between genes and proteins, elements and reactions, medications and illnesses, and organisms and cells (Kanehisa et al 2019 ). Parallel studies were reported in Vatica mangachapoi (Tang et al 2022 ), Hopea hainanensis (Huang et al 2022 ), Neesia altissima (Pratiwi et al 2022 ), C. capsularis (Satya et al 2017 ), Hibiscus hamabo siebold & zuccarini (Wang et al 2021 ), Abelmoschus esculentus (Nieuwenhuis et al 2021 ), Helicoverpa armigera (de la Paz Celorio-Mancera et al 2011 ), Gasterophilus nasalis (Zhang et al 2021 ), and Operculina turpethum (Biswal et al 2021 ). Additionally, GAEV was used to annotate the KO (Iacobas et al 2019 ; Emami-Khoyi et al 2020 ; Nand et al 2020 ; Shah et al 2021 ).…”

Section: Discussionmentioning

confidence: 53%

Genome-wide characterization leading to simple sequence repeat (SSR) markers development in Shorea robusta

Mishra

Meena

Kant

et al. 2023

Funct Integr Genomics

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Tropical rainforests in Southeast Asia are enriched by multifarious biota dominated by Dipterocarpaceae. In this family, Shorea robusta is an ecologically sensitive and economically important timber species whose genomic diversity and phylogeny remain understudied due to lack of datasets on genetic resources. Smattering availability of molecular markers impedes population genetic studies indicating a necessity to develop genomic databases and species-specific markers in S. robusta . Accordingly, the present study focused on fostering de novo low-depth genome sequencing, identification of reliable microsatellites markers, and their validation in various populations of S. robusta in Uttarakhand Himalayas. With 69.88 million raw reads assembled into 1,97,489 contigs (read mapped to 93.2%) and a genome size of 357.11 Mb (29 × coverage), Illumina paired-end sequencing technology arranged a library of sequence data of ~ 10 gigabases (Gb). From 57,702 microsatellite repeats, a total of 35,049 simple sequence repeat (SSR) primer pairs were developed. Afterward, among randomly selected 60 primer pairs, 50 showed successful amplification and 24 were found as polymorphic. Out of which, nine polymorphic loci were further used for genetic analysis in 16 genotypes each from three different geographical locations of Uttarakhand (India). Prominently, the average number of alleles per locus ( N a), observed heterozygosity ( H o), expected heterozygosity ( H e), and the polymorphism information content (PIC) were recorded as 2.44, 0.324, 0.277 and 0.252, respectively. The accessibility of sequence information and novel SSR markers potentially enriches the current knowledge of the genomic background for S. robusta and to be utilized in various genetic studies in species under tribe Shoreae. Supplementary Information The online version contains supplementary material available at 10.1007/s10142-023-00975-8.

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Section: Discussionmentioning

confidence: 53%

Genome-wide characterization leading to simple sequence repeat (SSR) markers development in Shorea robusta

Mishra

Meena

Kant

et al. 2023

Funct Integr Genomics

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“…Changes in the morphology of hyphae and microconidia after treatment with medium are thought to occur due to the bioactive compounds contained therein. The first research reported that the P. azotoformans UICC B-91 medium solution contains alkaloids [9]. Alterations in the structure of hyphae and microconidia occur after administration of alkaloid compounds that are able to disrupt cell membranes, causing cell lysis and the release cell components [31].…”

Section: Discussionmentioning

confidence: 99%

“…After 12 days of incubation, the results showed that T. rubrum did not grow after being inoculated into P. aeruginosa culture. In a before study reported that testing of P. azotoformans UICC B-91 medium solution against C. albicans ATCC 10231 was carried out using the disc diffusion method [9]. Medium with a concentration of 10 mg/mL and 50 mg/mL had an average zone of inhibition value of 11.50 ± 0.71 mm and 13.25 ± 0.35 mm, respectively.…”

Section: Introductionmentioning

confidence: 99%

Antifungal effect of Pseudomonas azotoformans UICC B-91 metabolites on hyphal growth of Trichophyton mentagrophytes

Pratiwi,

Putri,

Mangunwardoyo

et al. 2023

IOP Conf. Ser.: Earth Environ. Sci.

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Pseudomonas azotoformans UICC B-91 is known to have antimicrobial potential. The purpose of this study was to determine the inhibition mechanism of the metabolites of P. azotoformans UICC B-91 on the activity of Trichophyton mentagrophytes through morphological observations. The results showed that the metabolites produced by P. azotoformans UICC B-91 were inhibited growth of T. mentagrophytes. The zone of inhibition produced by the extract solution of P. azotoformans UICC B-91 against T. mentagrophytes at concentrations of 100 mg/mL and 80 mg/mL showed strong inhibitory power, with average values of 17.82 ± 0.32 mm and 10.79 ± 0.74 mm, respectively. The microscopic morphology of T. mentagrophytes after treatment with 0.1 g of ethyl acetate extract of P. azotoformans UICC B-91 showed wider hyphae compared to the control hyphae; abnormal cytoplasm; and granular structures between the hyphae, attached to the lateral side of the hyphae, and between the cell wall and the cell membrane. The mechanism of inhibition of the metabolites from the ethyl acetate extract of P. azotoformans UICC B-91 against T. mentagrophytes is thought to involve interference with cell walls and the function of the cell membrane.

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“…The selected bioactive compound (Table 1) based on previous fractionation and identification of the extract by thin-layer chromatography (TLC) and liquid chromatography-mass spectrophotometry (LCMS-MS). They detected several compounds of P. azotoformans UICC B-91, including futoamide (C 18 H 23 NO 3 ), gentialutine (C 9 H 11 NO), gentiabetine (C 9 H 11 NO 2 ),1-[(2E,4E)-2,4-decadienoyl] pyrrolidine (C 14 H 23 NO), lycopodine (C 16 H 25 NO) and dihydrolycopodine (C 16 H 27 NO) (Pratiwi et al, 2022).…”

Section: Sample Preparationmentioning

confidence: 99%

“…Based on phylogenetic analysis of nucleotide sequence generated from 16S rRNA region, two endophytic bacteria isolates isolated from N. altissima determined as P. aeruginosa and one isolate belongs to P. azotoformans (Pratiwi et al, 2016). (Pratiwi et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

In Silico Studies of Bioactive Compounds from Pseudomonas azotoformans UICC B-91 in Inhibiting Candida albicans

Pratiwi,

Sulistyaniningsih,

Jepri

2024

Jurnal Biodjati

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Futoamide, Gentialutine, Gentiabetine, 1-[(2E,4E)-2,4-dec- adienoyl] pyrrolidine, Lycopodine, Dihydro-lycopodine are bioactive compounds that can be obtained Pseudomonas azotoformans UICC B-91. Previous research results indicate that P. azotoformans extract- ed with dichloromethane, chloroform, and ethyl acetate have antican- didal activity against Candida albicans ATCC 10231. C. albicans is considered to become an opportunistic pathogen and well-known as the main cause of candidiasis. This study aims to determine the mech- anism of inhibition of bioactive compounds from P. azotoformans UICC B-91 on the growth of C. albicans using the molecular docking method. Docking was carried out using the targeted (Lanosterol 14 alpha demethylase and Glucan endo-1,3-beta-D-glucosidase) dock- ing method with an exhausted parameter of 50. The size of the grid- box was adjusted to the position of the amino acid residues based on predictions of binding sites using PrankWeb. The docking results were obtained in the form of binding affinity resulting from the inter- action of the compound with the protein. Results showed that the three sample compounds had the potential to form strong and stable bonds with both protein targets with only two ligands show a binding energy value of less than -7 kcal/mol. In addition, the speed and stability of the bond between the sample and the target protein cannot exceed control ligands, thus it can be predicted the structure of ligand. Fu- toamide, 1-[(2E,4E)-2,4-decadienoyl] pyrrolidine, Lycopodine, and Dihydrolycopodine had binding potential with Lanosterol 14 alpha demethylase. For Glucan endo-1,3-beta-D-glucosidase, only Fu- toamide had the potential to form stable and strong bonds that similar to the control. It can be concluded that the futoamide, one of ligand from P. azotoformans compounds has the potential as a multitarget inhibitor of the two C. albicans proteins, because it has various affini- ties and interaction stability for Lanosterol 14 alpha demethylase and Glucan endo-1,3-beta-D-glucosidase.

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Antimicrobial Compound from Endophytic Pseudomonas azotoformans UICC B-91 of Neesia altissima (Malvaceae)

Cited by 6 publications

References 30 publications

Genome-wide characterization leading to simple sequence repeat (SSR) markers development in Shorea robusta

Genome-wide characterization leading to simple sequence repeat (SSR) markers development in Shorea robusta

Antifungal effect of Pseudomonas azotoformans UICC B-91 metabolites on hyphal growth of Trichophyton mentagrophytes

In Silico Studies of Bioactive Compounds from Pseudomonas azotoformans UICC B-91 in Inhibiting Candida albicans

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