Antimicrobial Effect of Chitosan Nanoparticles on Streptococcus mutans Biofilms

Paz, Luis E. Chávez de; Resin, Anton; Howard, Kenneth A.; Sutherland, Duncan S.; Wejse, Peter Langborg

doi:10.1128/aem.02941-10

Cited by 196 publications

(102 citation statements)

References 14 publications

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“…The inhibitory effect of coating without antibiotic was not observed in the in vivo study, likely as a result of the competing effects of protein and cell adhesion [3,78,79]. Novel nanoparticle systems releasing antibiotics or antimicrobial silver have been shown to similarly inhibit bacterial growth and biofilm formation, but balancing bactericidal efficacy against cytotoxicity to the surrounding cells must be a consideration before clinical use [2,7,16,39].…”

Section: Discussionmentioning

confidence: 99%

Novel Antibiotic-loaded Point-of-care Implant Coating Inhibits Biofilm

Jennings

Carpenter

Troxel

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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Background Orthopaedic biomaterials are susceptible to biofilm formation. A novel lipid-based material has been developed that may be loaded with antibiotics and applied as an implant coating at point of care. However, this material has not been evaluated for antibiotic elution, biofilm inhibition, or in vivo efficacy.Questions/purposes (1) Do antibiotic-loaded coatings inhibit biofilm formation? (2) Is the coating effective in preventing biofilm in vivo? Methods Purified phosphatidylcholine was mixed with 25% amikacin or vancomycin or a combination of 12.5% of both. A 7-day elution study for coated titanium and stainless steel coupons was followed by turbidity and zone of inhibition assays against Staphylococcus aureus and Pseudomonas aeruginosa. Coupons were inoculated with bacteria and incubated 24 hours (N = 4 for each test group). Microscopic images of biofilm were obtained. After washing and vortexing, attached bacteria were counted. A mouse biofilm model was modified to include coated and uncoated stainless steel wires inserted into the lumens of catheters inoculated with a mixture of S aureus or P aeruginosa. Colony-forming unit counts (N = 10) and scanning electron microscopy imaging of implants were used to determine antimicrobial activity. Results Active antibiotics with colony inhibition effects were eluted for up to 6 days. Antibiotic-loaded coatings inhibited biofilm formation on in vitro coupons (log-fold reductions of 4.3 ± 0.4 in S aureus and 3.1 ± 0 for P aeruginosa in phosphatidylcholine-only coatings, 5.6 ± 0 for S aureus and 3.1 ± 0 for P aeruginosa for combinationloaded coatings, 5.5 ± 0.3 for S aureus in vancomycinloaded coatings, and 3.1 ± 0 for P aeruginosa for amikacinloaded coatings (p \ 0.001 for all comparisons of antibiotic-loaded coatings against uncoated controls for both bacterial strains, p \ 0.001 for comparison of antibioticloaded coatings against phosphatidylcholine only for S aureus, p = 0.54 for comparison of vancomycin versus combination coating in S aureus, P = 0.99 for comparison of antibiotic-and unloaded phosphatidylcholine coatings in P aeruginosa). Similarly, antibiotic-loaded coatings reduced attachment of bacteria to wires in vivo (log-fold

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Section: Discussionmentioning

confidence: 99%

Novel Antibiotic-loaded Point-of-care Implant Coating Inhibits Biofilm

Jennings

Carpenter

Troxel

et al. 2015

Clinical Orthopaedics &Amp; Related Research

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“…To date there is enough evidence to support that chitosan molecular mass can influence the solubility and its antibacterial activity. 5,29,30 Wound healing and hemostase During invasive dental treatments can occur bleeding disorders and dentists must be ready for it. To get the most effective way to control bleeding and wound healing, the best and popular solution is to use hemostatic agent.…”

Section: Bioactivitymentioning

confidence: 99%

“…Toothpastes, mouthwashes and chewing gums based on chitosan and herbs fullness functions antimicrobial effect on oral bio film and reduction of the number of S. mutans in the oral cavity. 10,29 Chitosan complex and fluoride micro particles increase fluoride absorption and protection cavities. Endodontic cements based on chitosan reduces inflammation and support bone regeneration.…”

mentioning

confidence: 99%

Chitosan-Properties and Applications in Dentistry

Kmiec

Pighinelli

et al. 2017

ATROA

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Chitosan is a biopolymer that has a large wound management application and biological proprieties, helping the organisms with fast healing, stimulates the cell proliferation including bacteriostatic and fungistatic particularly useful for wound treatment and as a support material to tissue engineering. The possibilities of application of chitosan are so huge and fascinating as well as not quite discovered. Proprieties of chitosan like biocompatibility, anti inflammatory and others could give promising results in periodontal care or wound healing after teeth extractions. The aim of this work is to review possible applications of chitosan in dentistry area.Keywords: chitosan, biomedical, bioengineering materials, biodegrability, selective permeability, polyelectrolyte action, natural polysaccharide Advances in Tissue Engineering and Regenerative Medicine Research Article Open AccessChitosan-properties and applications in dentistry by 12 Since then several researches with interests in the applications of chitin and aiming to broaden the knowledge about the structural relations and properties of this polysaccharide and its derivatives. 13 Chitosan is a straight chain, cationic polysaccharide that occurs naturally or can be obtained by deacetylation of chitin. Even though there is no nomenclature that definitively guarantees a difference between chitin and chitosan, the term chitosan usually represents cationic copolymers consisting of 2-amino-2-deoxy-β-D-glucose (60-100%) and 2-acetamino -2-deoxy-β-D-glucoside (0-50%), bound together by ß (1→4) bonds. [14][15][16][17] Chitosan is the main derivative of chitin deacetylation in which the mean degree of deacetylation representing the percentage of free NH 2 groups is greater than 60%. 18,19 The average degree of acetylation of chitosan is a measure of the average number of 2-acetamide-2-dexosi-D-glucopyranose and 2-amino-2-deoxy-D-glucopyranose units. The relative percentage of these units has a direct influence on the solubility of chitosan and is an important parameter to determine the average degree of acetylation or indirectly the average degree of deacetylation, which in this way represents the concentration of amino groups, besides exerting a great influence on the physical properties, chemical and biological. 11The amino groups of chitosan are more reactive with respect to the acetamido groups of chitin. The free electron pair of nitrogen in the amino groups is responsible for the adsorption of metallic cations. The average degree of deacetylation determines the fraction of amino groups that are available for interaction with metals. The protonation of amino groups in acid solutions is responsible for the electrostatic attraction of anions. 14 Unlike chitin, chitosan is soluble in dilute acid medium forming a cationic polymer that confers special properties differentiated with respect to the vegetal fibers.11 Chitosan is soluble in dilute acids, such as acetic acid, formic acid, lactic acid, as well as inorganic acids, after prolonged agitation. However,...

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“…[44][45][46][47][48][49][50] Além disso, recentemente, pesquisas mostraram sua capacidade de combater biofilmes bacterianos e fúngicos. 11,[47][48][49][50] Uma limitação bastante conhecida dos polímeros de quitosana (sobretudo os de alta massa molecular) é que eles são insolúveis em soluções aquosas a pH fisiológicos (pKa amina ≈6,3 < pH fisiológico ≈7,0), tornando restrita a sua aplicação somente para soluções que tenham pHs mais ácidos (pH ácido < 6,0), em que seus grupos amina ficam protonados (NH2  (NH3) + ), conferindo uma carga positiva ao polímero. [44][45][46] Contudo, já há grupos de pesquisa que estão tentando aumentar a solubilidade de quitosanas em pHs neutros.…”

Section: Motivaçãounclassified

“…Por exemplo, a adição de trifosfato de sódio polianiônico (TPP) em nanopartículas de quitosana consegue estabilizá-las em pH neutro, sendo até eficazes contra biofilmes bacterianos de Streptococcus mutans. 50 Logo, acreditamos que quitosanas e/ou seus derivados poderão ser excelentes transportadores de compostos antimicrobianos, capazes de combater diferentes tipos de biofilmes bacterianos. Em particular, a estratégia de usar nanopartículas de quitosana como veículo de entrega de fotossensibilizadores (FS) poderia ser uma das mais promissoras no combate de biofilmes bacterianos e de microrganismos resistentes a antibióticos.…”

Section: Motivaçãounclassified

Mecanismos de interação molecular de polieletrólitos antimicrobianos em membranas modelo por espectroscopia vibracional não linear

Rimoli¹

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AGRADECIMENTOSA Deus, por tudo e por cada segundo de vida.A toda minha família, pelo amor, exemplo, suporte e compreensão em todos os momentos. Em especial: Pai, obrigado por me ensinar a ser paciente e organizado; Mãe, obrigado por me ensinar a trabalhar com amor e determinação; Maninha, obrigado por nunca me deixar esquecer que é a companhia das pessoas queridas que faz a vida valer a pena. A todos os docentes dos quais fui aluno -muito obrigado pela dedicação e paciência.Aos funcionários do Serviço da Pós-Graduação do IFSC, Silvio, Ricardo e Priscila, pelo profissionalismo, paciência e atenção.Às meninas da biblioteca, Cris, Neusa e Ana, por sempre serem muito atenciosas, Enfim, a todos os que estiveram presentes nesta longa trajetória, que eu já tenha mencionado ou não: saibam que eu sou um pedacinho de cada um de vocês e eu sou muito grato pela nossa convivência e experiência de vida compartilhada. showed that both PAH and CO led to a small expansion of DPPC monolayers. However, for DPPG monolayers both polyelectrolytes led to significant expansion, with CO causing a more dramatic effect. SFG spectra in the CH stretch range showed that the lipid chain conformation remained always well ordered in all cases (slightly less ordered upon interacting with PAH), despite membrane expansion. This indicates that CO were inserted in the monolayer, forming islands of CO within the lipid film. Changes in the SFG spectral lineshape of OH stretches for the interfacial water molecules indicated that PAH adsorption on both DPPC and DPPG films was able to overcompensate the lipid negative charge and led to an overall surface charge reversal. The SFG spectra of the phosphate groups also indicated that in pure water the DPPC headgroups had a more ordered orientation than in the case of DPPG. Nevertheless, upon interaction with the cationic polyelectrolytes, the DPPG headgroups also become ordered, with a preferential orientation towards the subphase. Experiments with the antimicrobials injected in the subphase under a condensed Langmuir film indicated that CO were also capable of monolayer penetration, albeit causing a reduced film expansion. This comparison indicates that the choice of experimental methodology affects the outcome, but both may be complementary, as they may represent different phases of a biomembrane lifecycle.The detailed view provided here for the molecular interaction of these polyelectrolytes with lipid films may shed light on the mechanism of their biocidal activity and aid on a rational design of new antimicrobial polymers.

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Antimicrobial Effect of Chitosan Nanoparticles on Streptococcus mutans Biofilms

Cited by 196 publications

References 14 publications

Novel Antibiotic-loaded Point-of-care Implant Coating Inhibits Biofilm

Novel Antibiotic-loaded Point-of-care Implant Coating Inhibits Biofilm

Chitosan-Properties and Applications in Dentistry

Mecanismos de interação molecular de polieletrólitos antimicrobianos em membranas modelo por espectroscopia vibracional não linear

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