Antimicrobial Effect of Coated Leather Based on Silver Nanoparticles and Nanocomposites: Synthesis, Characterisation and Microbiological Evaluation

Abstract

This paper describes the assessment of the antimicrobial effect of leathers employed for footwear production, which were obtained by different tanning techniques and treated with silver nanoparticles emulsions synthesised from different reducing agents (sodium borohydride, gelatine/glucose and Aloe vera extract), as well as the assessment of the silica nancomposites obtained from said nanoparticles. After the characterisation of silver nanoparticles and nanocomposites, the emulsions were applied onto different leather samples. Scanning Electron Microscopy was used to verify that silver nanoparticles were deposited on the collagen fibres surface and inside the collagen matrix of leather. The antibacterial effect of different silver nanoparticles and silver nanocomposites was assessed on gram positive and gram negative bacterial strains in agar diffusion assays and turbidimetric tests. The results obtained proved a powerful antibacterial effect on all bacterial strains tested of both silver nanoparticles and silica nanocomposites. This allows us to consider the incorporation of these nanocomposites into leather as an alternative to other commercially available products in order to obtain leather with antimicrobial properties that confer better functionality and added value.

Aplikasi Nanopartikel Perak dengan Bioreduktor Daun Sambiloto (Andrographis Peniculata) dan Kulit Pisang Kepok (Musa Paradisiaca L) sebagai Antibakteri pada Kulit

“…Beberapa penelitian penggunaan nanopartikel perak pada kulit telah dilakukan diantaranya penggunaan Nano-Ag-TiO2 untuk pelapisan (coating) pada kulit lapis (Kayguzuz et al, 2016) dan penggunaan nanopartikel perak untuk insock, lining dan upper material (Lopez et al, 2015) dan kulit berbulu (Perumalraj, 2012). Karakterisasi dan uji performance material kulit menggunakan nano partikel perak dilakukan oleh (Lkhagvajav et al, 2015).…”

“…Karakterisasi nano partikel Ag menggunakan spektroskopi dan studi keamanan Nano partikel Ag sebagai antibakteri pada kulit telah dilakukan oleh (Sportelli et al, 2017). Lopez et al, (2015) melakukan penelitian tentang Pengaruh coating nanopartikel perak sebagai antibakteri dari sintesis, karakterisasi dan evaluasi mikrobiologi. Studi tentang peningkatan sifat permukaan kulit yang dicoating dengan nanopartikel perak (Ag NPs) telah dilakukan oleh Elsayed et al, (2021).…”

“…Interaksi antara kulit tersamak dengan nanopartikel perak diduga karena adsorpsi kimia, yaitu melalui ikatan kimia (ikatan kovalen) antara -NH, -COOH, -NHCOO-dan Ag (Maneerung et al, 2008). Lopez et al, (2015) melakukan analisis serat kolagen dari kulit yang ditreatment dengan nanopartikel perak menggunakan SEM, tampak adanya titik-titik (dot) berwarna putih terang pada permukaan serat kolagen kulit. Hal ini menunjukkan terjadinya penetrasi nanopartikel perak (AgNPs) pada serat kolagen kulit.…”

Hybrid Silica Xerogel and Titania/Silica Xerogel Dispersions Reinforcing Hydrophilicity and Antimicrobial Resistance of Leathers

“…Therapeutic applications include catheters [11] and wound dressings [12]. Several studies have reported the application of AgNPs to leathers as colloidal solutions and emulsions [13,14] and through microencapsulation [15].…”

Development of antimicrobial leather modified with Ag–TiO2 nanoparticles for footwear industry

“…However, very few reports on the toxicity of Ag-NPs are available, which show different degrees of in vitro cytotoxicity [7,8]. Several studies have reported the application of Ag-NPs in leathers, as colloidal solutions and emulsions of Ag-NPs [9,10], via Ag-NPs microencapsulation [11], and Ag-NPs synthesis using natural polymers such pine resin or gum [12].…”