Antimicrobial Effect of Ozone Made by KP Syringe of HighFrequency Ozone Generator

Abstract

Svrha ovog istraživanja bila je procijeniti in vitro učinkovitost ozona na suspenziju triju različitih vrsta bakterija inokluiranih u obrađene korijenske kanale ekstrahiranih ljudskih zubi. Materijal i metode: Ozon je proizveden od aspiriranog zraka uporabom specijalne KP brizgalice visokofrekvencijskoga generatora ozona Ozonytron (Biozonix, München, Njemačka) s pomoću dielektričnog barijernog izboja, te nastavka na štrcaljki postavljenog u pripremljeni korijenski kanal. Ispitivani su mikroorganizmi Enterococcus faecalis, Staphylococcus aureus i Staphyloccocus epidermidis. Rezultati: Ni jedna metoda nije pokazala 100-postotnu učinkovitost u eliminaciji navedenih bakterija u suspenziji. Primjena ozona značajno je smanjila ukupan broj mikroorganizama (89,3 %), te broj svake vrste bakterija posebno (Staphyloccocus aureus-94,0 %; Staphyloccocus epidermidis-88,6 % i Enterococcus faecalis-79,7 %). Ozon proizveden KP brizgalicom bio je statistički znatno učinkovitiji u eliminaciji Staphyloccocus aureus i Staphyloccocus epidermidis u usporedbi s NaOCl-om kao pozitivnom kontrolom. Zaključak: Ukupan broj Enterococcus faecalis bio je statistički manji, no bez značajne statističke razlike između ispitivane i pozitivne kontrolne skupine. Između triju vrsta bakterija u suspenziji, primjena KP štrcaljke pokazala je najmanju učinkovitost u eliminaciji Enterococcus faecalis.

Analysis of electric field and emission spectrum in the glow discharge of therapeutic plasma electrode

“…using ozone-producing syringe as described in our recent paper [13], the therapeutic effects can indeed be ascribed exclusively to ozone [27]. Similarly, when ozone is produced through DBD and CAP generation, in the air gap between plasma electrode and treated surface as reported by Tuncer Figure 1.…”

“…A clinical evaluation of such method indicates positive results in disinfection [13][14][15] post-surgical soft tissue and bone healing and pain relief [16][17][18][19][20], treatment of various oral and dental diseases [21][22][23][24], reduction of temporomandibular disorder-related pain [25], and bisphosphonate-related osteonecrosis of the jaw [26]. In recent available literature found on the clinical application of plasma electrode coupled with high-frequency generator, all of the reported therapeutic effects are generally ascribed to bio-oxidative effects of ozone [14][15][16][17][18][19][20][21][22][23][24][25][26].…”

High-Frequency Generator in Wound Healing of Marjolin's Ulcer After Carcinoma Resection

“…6 HFG uses ozone as mechanism of action, which has germicide, bactericide, and antiseptic properties by stimulating the oxygen metabolism and activating the immune system. 4,[6][7][8] These effects were considered on the choice of a safe physical agent to apply over the wounds that once already had cancer, 7,9 and furthermore, it helped in tissue repair, wound closure/reduction, and prevention of infections. 4,[6][7][8] The case report presented had percentage of reduction about 84% considering all wounds, 68% to wound 3 and 56.41% to wound 1, which was similar to Korelo et al 6 with 68.27%, but in our case a longterm treatment was necessary.…”

“…The main effects of the physical agents are that they can stimulate cellular metabolism, cellular growth, and angiogenesis, [4][5][6][7] but there is no consensus if they can stimulate tumor growth in humans, as well. 3,5,9 However, several benefits have been showed to cancer patients, as reduction of pain, mucositis, necrosis, hematoma, and lymphedema.…”

“…The formation of these atomic oxygen molecules influences the biochemical events of cellular metabolism and promotes antimicrobial, bactericide and fungicide effects. 4,6,7 These properties accelerate the tissue repair and can control the infection process as showed by some experimental 4,7 and clinical studies 6,8 described on Table 1.…”