Antimicrobial Effect of Photosensitized Rose Bengal on Bacteria and Viruses in Model Wash Water

Cossu, Andrea; Ercan, Duygu; Tikekar, Rohan V.; Nitin, Nitin

doi:10.1007/s11947-015-1631-8

Cited by 26 publications

(19 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The equipment consisted of four UV-A light fluorescent bulbs (320-400 nm, peak wavelength 360 nm, 18 Watt, Actinic BL, Royal Philips, the Netherlands) mounted on the ceiling of a closed box. A more detailed description of the system is reported in a previous study (Cossu et al, 2016). The intensity of the UV-A light applied to the surface of the sample placed in the equipment was 2646 ± 212 µW/cm 2 as measured with the radiometer model UV-340A (Lutron, Taipei, Taiwan).…”

Section: Uv-a Light Devicementioning

confidence: 99%

“…Although photosensitizers (PS) are widely used for the sanitation in biomedical field (Luksiene and Brovko, 2013), their application to sanitize process water used during food processing has been only recently introduced (Cossu et al, 2016;Ercan et al, 2016). The generation of reactive oxygen species (ROS) by PS upon exposure to ultraviolet (UV) or visible (VIS) light and the subsequent killing of microorganisms can provide an effective alternative to address some of the key limitations of chemical sanitizers.…”

Section: Introductionmentioning

confidence: 99%

“…The generation of reactive oxygen species (ROS) by PS upon exposure to ultraviolet (UV) or visible (VIS) light and the subsequent killing of microorganisms can provide an effective alternative to address some of the key limitations of chemical sanitizers. Recent studies have demonstrated the effectiveness of food-grade photosensitizers with the synergistic effect of visible light (Cossu et al, 2016) or UV-A light (Ercan et al, 2016) for the treatment of wash water. These prior studies focused on the use of food-grade dyes or inorganic particles as PS agents for the inactivation of viral and bacterial targets in the presence of organic content in wash water.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Antimicrobial effect of synergistic interaction between UV-A light and gallic acid against Escherichia coli O157:H7 in fresh produce wash water and biofilm

Cossu

Ercan

Wang

et al. 2016

Innovative Food Science & Emerging Technologies

Self Cite

View full text Add to dashboard Cite

A synergistic interaction between gallic acid (GA) and UV-A light (UV-A+GA) to inactivate E. coli O157: H7 in spinach wash water and in biofilm was evaluated. A 30-min exposure to UV-A light in presence of 10 mM GA had relevant biological effect in the inactivation of E. coli O157:H7 in suspension in the absence (> 5 log(CFU/mL)) and the presence of organic content (>3log(CFU/mL) in 2,000 mg O 2 /L COD (Chemical Oxygen Demand) organic load), and resulted in ~80% decrease in the metabolic activity of E. coli O157:H7 biofilm. GA solutions could be recycled through at least 3-cycles of UV-A treatment without a significant loss in antibacterial effect. Catalase reduced the extent of E. coli O157:H7 inactivation from the UV-A+GA treatment suggesting that generation of hydrogen peroxide was predominantly responsible for the observed antimicrobial effect. The UV-A+GA treatment was also found to be effective in causing more than 3 log(CFU/mL) reductions in E. coli O157:H7 on the surface of spinach leaves. UV-A + GA treatment can serve as an effective intervention in the fresh produce sanitation.

show abstract

Section: Uv-a Light Devicementioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Antimicrobial effect of synergistic interaction between UV-A light and gallic acid against Escherichia coli O157:H7 in fresh produce wash water and biofilm

Cossu

Ercan

Wang

et al. 2016

Innovative Food Science & Emerging Technologies

Self Cite

View full text Add to dashboard Cite

show abstract

“…In comparison, UV-A plus LAE significantly (P Ͻ 0.05) increased nucleic acid release at 20 and 30 min, suggesting increased bacterial cell membrane permeabilization over time. The increased release of nucleic acid absorbance is expected since enhanced inactivation of cells by the combination of LAE and UV-A may result in increased permeability of the bacterial cell membrane (20). Fig.…”

Section: Resultsmentioning

confidence: 99%

“…Since LAE was reported as a membrane-disrupting antimicrobial compound (13,14), damage to bacterial cell membrane was analyzed in the synergistic combination. A disrupted cell membrane structure will usually lead to the diffusion of small molecules, including potassium ions and nucleic acid, from the cytoplasm to the outer environment (20,38). E. coli was chosen as a model microorganism for the results presented in Fig.…”

Section: Methodsmentioning

confidence: 99%

Synergistic Antimicrobial Activity by Light or Thermal Treatment and Lauric Arginate: Membrane Damage and Oxidative Stress

Rai

Huu

et al. 2019

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

The need for more effective antimicrobials is critical for the food industry to improve food safety and reduce spoilage of minimally processed foods. The present study was initiated to develop an efficient and novel antimicrobial approach which combines physical treatments (UV-A or mild heat) and generally recognized as safe lauroyl arginate ethyl (LAE) to inactivate surrogate strains, including Escherichia coli and Listeria innocua. Synergistic inactivation of bacteria resulted in an ∼6-log reduction of target bacteria, while individual treatments resulted in <1.5-log inactivation under the same set of conditions. In addition, the synergistic mechanism between LAE and UV-A/mild heat was evaluated by supplementing with a variety of antioxidants for suppressing oxidative stress and measurement of cell membrane damage by nucleic acid release. These results demonstrate that the synergistic antimicrobial activity of LAE and mild physical stresses was suppressed by supplementation with antioxidants. The research also compared LAE with another membrane-targeting lipopeptide antimicrobial agent, polymyxin B, to understand the uniqueness of LAE-induced synergy. Briefly, differences in modes of action between LAE and polymyxin B were characterized by comparing the MIC, damage to liposomes, and oxidative stress generation. These differences in the mode of action between LAE and polymyxin B suggested that both compounds target cell membrane but significantly differ in mechanisms, including membrane disruption and oxidative stress generation. Overall, this study illustrates synergistic antimicrobial activity of LAE with light or mild heat and indicates a novel oxidative stress pathway that enhances the activity of LAE beyond membrane damage. IMPORTANCE This study highlights an effective antimicrobial processing approach using a novel combination of lauroyl arginate ethyl (LAE) and two different physical treatments, light (UV-A) and mild heat. Both combinations demonstrated synergistic inactivation against a model Gram-negative bacterium or a Gram-positive bacterium or both by a >5-log reduction. Further mechanistic study revealed that oxidative stress is responsible for synergistic inactivation between LAE and UV-A, while both membrane damage and oxidative stress are responsible for the synergistic combination between LAE and mild heat. The mode of action of LAE was further compared to that of polymyxin B and analyzed using artificial membrane model systems and the addition of antioxidants. The proposed combination of LAE and common physical treatments may improve food preservation, food safety, and current sanitation processes for the food industry and the inactivation of pathogenic strains in biomedical environments.

show abstract

Visible‐Light‐Driven and Self‐Hydrogen‐Donated Nanofibers Enable Rapid‐Deployable Antimicrobial Bioprotection

Chang

et al. 2021

Small

View full text Add to dashboard Cite

The novel coronavirus SARS‐CoV‐2 has prompted a worldwide pandemic and poses a great threat to public safety and global economies. Most present personal protective equipment (PPE) used to intercept pathogenic microorganisms is deficient in biocidal properties. Herein, we present green nanofibers with effective antibacterial and antiviral activities that can provide sustainable bioprotection by continuously producing reactive oxygen species (ROS). The superiority of the design is that the nanofibers can absorb and store visible light energy and maintain the activity under light or dark environment. Moreover, the nanofibers can uninterruptedly release ROS in the absence of an external hydrogen donor, acting as a biocide under all weather conditions. A facile spraying method is proposed to rapidly deploy the functional nanofibers to existing PPE, such as protective suits and masks. The modified PPE exhibit stable ROS production, excellent capacity for storing activity potential, long‐term durability, and high bactericidal (>99.9%) and viricidal (>99.999%) efficacies.

show abstract

Antimicrobial Effect of Photosensitized Rose Bengal on Bacteria and Viruses in Model Wash Water

Cited by 26 publications

References 39 publications

Antimicrobial effect of synergistic interaction between UV-A light and gallic acid against Escherichia coli O157:H7 in fresh produce wash water and biofilm

Antimicrobial effect of synergistic interaction between UV-A light and gallic acid against Escherichia coli O157:H7 in fresh produce wash water and biofilm

Synergistic Antimicrobial Activity by Light or Thermal Treatment and Lauric Arginate: Membrane Damage and Oxidative Stress

Visible‐Light‐Driven and Self‐Hydrogen‐Donated Nanofibers Enable Rapid‐Deployable Antimicrobial Bioprotection

Contact Info

Product

Resources

About