Antimicrobial Effects of Ethanol, Methanol and Ethyl Acetate Teucrium polium and Citrullus colocynthis extract on Pseudomonas aeruginosa

Shahraki-Mojahed, Laleh; Behzadmehr, Rezvaneh; Beigomi, Zahra

doi:10.54458/mev.v1i01.6671

Cited by 8 publications

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“…The methanol extracts of the plant showed strong antibacterial activity against Bacillus subtillis , Streptococcus pyogenes , and Salmonella typhi [ 29 ]. Besides, the aqueous and acetone extracts of C. colocynthis Schrad (roots, stems, leaves, and three stages of its fruit and seeds) showed antibacterial activities against all tested bacteria [ 30 , 31 ]. The high quantities of phenolic compounds were reported in the aqueous and ethanolic extracts of the plant C. colocynthis .…”

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confidence: 99%

Isolation and identification of endophytic actinobacteria from Citrullus colocynthis (L.) Schrad and their antibacterial properties

et al. 2022

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Background Antibiotic resistance poses a major threat to human health globally. Consequently, new antibiotics are desperately required to discover and develop from unexplored habitats to treat life-threatening infections. Microbial natural products (NP) are still remained as primary sources for the discovery of new antibiotics. Endophytic actinobacteria (EA) which are well-known producers of bioactive compounds could provide novel antibiotic against pathogenic bacteria. This research aimed to isolate EA from the Citrullus colocynthis plant and explore the antibacterial properties of their metabolites against pathogenic bacteria. Results The healthy samples were collected, dissected and surface-sterilized before cultured on four different selection media at 28 °C. Six endophytic actinobacteria were isolated from Citrullus colocynthis plant. They were taxonomically classified into two family namely Streptomycetaceae and Nocardiopsaceae, based on colony morphological features, scanning electron microscope analysis and molecular identification of isolates. This is the first report on the identification of EA form Citrullus colocynthis and their antibacterial activity. The strains generated a chain of vibrio-comma, cubed or cylindrical shaped spores with indenting or smooth surfaces. Three of those were reported as endophytes for the first time. The strain KUMS-C1 showed 98.55% sequence similarity to its closely related strains which constitutes as a novel species/ strain for which the name Nocardiopsis colocynthis sp. was proposed for the isolated strain. Five isolated strains had antagonist activity against S. aureus, P. aeruginosa, and E. coli. Among those, stain KUMS-C6 showed the broadest spectrum of antibacterial activity against all test bacteria, whereas the strain KUMS-C4 had no antibacterial activity. Conclusions NPs have a long history of safe and efficient use for development of pharmaceutical products. Our study highlights that Citrullus colocynthis is an untapped source for the isolation of EA, generating novel and bioactive metabolites by which might lead to discovery of new antibiotic(s). This study reveals the future of new antibiotic developments looks bright against multi-drug resistance diseases by mining under- or unexplored habitats.

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Section: Introductionmentioning

confidence: 99%

Isolation and identification of endophytic actinobacteria from Citrullus colocynthis (L.) Schrad and their antibacterial properties

et al. 2022

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“…Many plant essential oils have been reported to have significant inhibitory effects on pathogenic microorganisms, and it has been shown that most herb essential oils obtained from medicinal herbs have antifungal, antiparasitic, antibacterial, and antimicrobial properties; therefore, plant essential oils have been greatly utilized in some fields such as pharmacology, herbal pharmacology, pharmaceutical/clinical microbiology, and phytopathology, as well as maintenance of food, fruits, and vegetables (3,4). These herbal medicines are popular among people (5).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Antimicrobial Activity of Several Medicinal Plants Against Clinically Isolated Staphylococcus aureus from Humans and Sheep

et al. 2021

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Background: Continuous and indiscriminate use of chemical drugs causes an important phenomenon of resistance to microorganisms. Accordingly, the effect of medications is minimized or offset, increasing drug use and the need to study mixtures with more latest and powerful formulations. On the other hand, it has been reported that many plants essential oils have a significant inhibitory effect on pathogenic microorganisms. Objectives: This study aimed to evaluate the antimicrobial activity of some curative herbs against some clinical bacteria of humans and sheep. Methods: The leaves of chicory (Cichorium intybus L.), Hypericum perforatum L., Lavandula angustifolia, Thymus vulgaris L., and Taxus baccata L. were collected and determined in the botanical laboratory of the University of Zabol. Forty grams of dried leaves was used in 400 mL of ethanol (96%) to prepare the ethanolic extract. DPPH was used to determine the activity of reactive oxygen species (ROS) trapping. The antimicrobial effects were studied by the disk diffusion (6 mm) method in Müller-Hinton agar medium according to the method by Bauer et al. Results: The minimum inhibitory concentrations (MICs) of chicory, thyme, H. perforatum, French lavender, and yarrow extracts in human clinical Staphylococcus aureus were 6.25, 12.5, 3.1, 25, and 6.25 ppm, respectively, but, in sheep, clinical S. aureus, were 12.5, 6.25, 3.1, 12.5, and 25 ppm, respectively. The minimum bactericidal concentrations (MBCs) of chicory, thyme, H. perforatum, French lavender, and yarrow extracts in human clinical S. aureus were 12.5, 25, 6.25, 50, and 12.5 ppm, respectively, but, in sheep clinical S. aureus, were 25, 12.5, 6.25, 25, and 50 ppm, respectively. The most effective extract in inhibiting the growth of S. aureus was the H. perforatum L. extract with an 8.9-mm diameter growth inhibition zone. Conclusions: Regarding the side effects of artificial medications and antimicrobials, as well as the significant influence of healing herb extracts used in this study, it was found that H. perforatum was the most effective plant against S. aureus. It should be noted that plant extracts were more effective in human clinical S. aureus than in sheep clinical S. aureus.

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Harnessing anti-infective efficacy of Cinnamomum verum in synergy with β-lactam and fluoroquinolones drugs to combat virulence and biofilms of Pseudomonas aeruginosa PAO1

Samreen,

Siddiqui,

Ahmad

2024

Microbial Pathogenesis

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Antimicrobial Effects of Ethanol, Methanol and Ethyl Acetate Teucrium polium and Citrullus colocynthis extract on Pseudomonas aeruginosa

Cited by 8 publications

References 0 publications

Isolation and identification of endophytic actinobacteria from Citrullus colocynthis (L.) Schrad and their antibacterial properties

Isolation and identification of endophytic actinobacteria from Citrullus colocynthis (L.) Schrad and their antibacterial properties

Evaluation of Antimicrobial Activity of Several Medicinal Plants Against Clinically Isolated Staphylococcus aureus from Humans and Sheep

Harnessing anti-infective efficacy of Cinnamomum verum in synergy with β-lactam and fluoroquinolones drugs to combat virulence and biofilms of Pseudomonas aeruginosa PAO1

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