Antimicrobial Efficacy of Photodynamic Therapy and Sodium Hypochlorite on Monoculture Biofilms of <i>Enterococcus faecalis</i> at Different Stages of Development

Zand, Vahid; Milani, Amin Salem; Amini, Mohd Hazim Mohamad; Barhaghi, Mohammad Hossein Soroush; Lotfi, Mehrdad; Rikhtegaran, Sahand; Sohrabi, Aydin

doi:10.1089/pho.2013.3557

Cited by 32 publications

(23 citation statements)

References 30 publications

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“…Longer incubation times for in vitro biofilm formation have been used more recently. Longer incubation times may result in a thicker biofilm, which may influence its susceptibility to antimicrobial agents [26,27]. However, in what way this could have influenced the results remains unknown.…”

Section: Discussionmentioning

confidence: 99%

The efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique

Oliveira

Aguiar

Câmara

et al. 2015

Photodiagnosis and Photodynamic Therapy

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Section: Discussionmentioning

confidence: 99%

The efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique

Oliveira

Aguiar

Câmara

et al. 2015

Photodiagnosis and Photodynamic Therapy

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“…Zand et al (23) observed that photodynamic therapy and the 2.5% NaOCL solution were equally efficient in completely eliminating E. faecalis in young, mature and old biofilms using 120 permanent human maxillary and mandibular central incisors. Sabino et al (24) contaminated ten permanent third molar with a bioluminescent strain of C. albicans and introduced into the canals blue methylene and irradiated with laser tip either in contact with pulp chamber or within the canal using an optical diffusion fiber.…”

Section: Discussionmentioning

confidence: 99%

Manual and Rotary Instrumentation Ability to Reduce Enterococcus faecalis Associated with Photodynamic Therapy in Deciduous Molars

et al. 2014

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This aim of this study was to assess the ability of manual or rotary instrumentation associated with photodynamic therapy (PDT) to reduce Enterococcus faecalis using three combinations of light/photosensitizers: toluidine blue O/laser, fuchsin/halogen light and fuchsin/LED. Twenty deciduous molars were selected and contaminated with Enterococcus faecalis (McFarland 0.5 scale). Working length determination was performed by visual method. The teeth were randomly divided into two groups: G1 (n=10): manual instrumentation (Kerr-type files) and G2 (n=10): rotary instrumentation (ProTaper system). The bacteria were collected three times using sterile paper cones compatible with the anatomic diameter of the root canal for 30 s before and after instrumentation and after PDT. The samples were diluted in peptone water, seeded on blood agar plates and incubated in an oven at 37 °C for colony-forming units counting. The decrease of E. faecalis counts after instrumentation and after PDT was compared using the Wilcoxon test, t-test and Kruskal Wallis test. A significant reduction of E. faecalis occurred after manual and rotary instrumentation and after PDT using the three combinations of light/ photosensitizer (p<0.05). It may be concluded that both rotary and manual instrumentation reduced E. faecalis. Fuchsin with halogen light or LED irradiation and toluidine blue O with laser irradiation can be used to reduce E. faecalis in root canals of primary molars. PDT can be used as an adjuvant to conventional endodontic treatment.

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“…However, in addition to the above-mentioned batch-culture studies, phenothiazinium derivatives have also been examined in more applied models: Zand et al (2014) used extracted human maxillary and mandibulary incisors, decoronated them and instrumented their root canals up to #60 K-file. After an autoclavation process Enterococcus faecalis biofilms were formed in these root canals for 4, 6 or 8 weeks.…”

Section: Apdt Against Biofilms In Vitromentioning

confidence: 99%

“…After an autoclavation process Enterococcus faecalis biofilms were formed in these root canals for 4, 6 or 8 weeks. Subsequently, canals were incubated with TBO (25 μg/ml) for 5 min and irradiated with a 625 nm diode laser (100 mW; light dose 214.28 J/cm 2 ) twice for 2.5 min intermitted by a 2.5 min break, applying a total of 2 × 15 J, which led to a complete elimination of Enterococcus faecalis below detection limit of the CFU assay (Zand et al, 2014). …”

Section: Apdt Against Biofilms In Vitromentioning

confidence: 99%

Antimicrobial photodynamic therapy for inactivation of biofilms formed by oral key pathogens

Cieplik¹,

Tabenski²,

Buchalla³

et al. 2014

Front. Microbiol.

201

137

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With increasing numbers of antibiotic-resistant pathogens all over the world there is a pressing need for strategies that are capable of inactivating biofilm-state pathogens with less potential of developing resistances in pathogens. Antimicrobial strategies of that kind are especially needed in dentistry in order to avoid the usage of antibiotics for treatment of periodontal, endodontic or mucosal topical infections caused by bacterial or yeast biofilms. One possible option could be the antimicrobial photodynamic therapy (aPDT), whereby the lethal effect of aPDT is based on the principle that visible light activates a photosensitizer (PS), leading to the formation of reactive oxygen species, e.g., singlet oxygen, which induce phototoxicity immediately during illumination. Many compounds have been described as potential PS for aPDT against bacterial and yeast biofilms so far, but conflicting results have been reported. Therefore, the aim of the present review is to outline the actual state of the art regarding the potential of aPDT for inactivation of biofilms formed in vitro with a main focus on those formed by oral key pathogens and structured regarding the distinct types of PS.

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Antimicrobial Efficacy of Photodynamic Therapy and Sodium Hypochlorite on Monoculture Biofilms of Enterococcus faecalis at Different Stages of Development

Cited by 32 publications

References 30 publications

The efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique

The efficacy of photodynamic therapy and sodium hypochlorite in root canal disinfection by a single-file instrumentation technique

Manual and Rotary Instrumentation Ability to Reduce Enterococcus faecalis Associated with Photodynamic Therapy in Deciduous Molars

Antimicrobial photodynamic therapy for inactivation of biofilms formed by oral key pathogens

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