Antimicrobial Efficacy of Triple Antibiotic Paste and Propolis as an Intracanal Medicament in Young Permanent Teeth: An In Vivo Study

Abstract

A bstract Aim and objective To evaluate the antimicrobial efficacy of triple antibiotic paste and propolis extracts as an intracanal medicament in young permanent teeth. Materials and methods A total of 30 single-rooted non-vital young permanent teeth with open apex were selected randomly from the children aged between 7 years and 14 years with no systemic complications. Group I—triple antibiotic paste and group II be propolis allocating 15 teeth in each group. After access opening, the first sample (S1) was collected by inserting paper point into the root canal, the second sample (S2) was collected immediately after irrigation, and the third sample (S3) was collected after post-intracanal medication after 3–4 weeks. Samples were sent for microbiological analysis to assess the bacterial count, and for the obtained data, statistical analysis was done. Results The mean colony count among the triple antibiotic paste group was 1906.75. After access opening, which was reduced to 315.12 after irrigation, and after 3–4 weeks, it was 817.25. There was a significant difference between sample 1, sample 2, and sample 3 ( p value = 0.008). The mean colony count among the propolis group was 1427.87 after access opening, which was reduced to 436.00 after irrigation, and after 3–4 weeks, it has reduced to 252.37. There was a significant difference between sample 1, sample 2, and sample 3 ( p value = 0.032). Intergroup comparison between the groups showed no statistical difference between the samples. Conclusion Propolis exhibited similar antimicrobial efficacy, which is comparable to triple antibiotic paste. So, propolis can be utilized as an intracanal medicament in young permanent teeth with an open apex. Clinical significance Propolis is a naturally occurring flavonoid-rich resinous product with antibacterial, antifungal, antiviral, immunomodulatory, and antioxidant effects. It is safe without any drug allergies and bacterial sensitivity and is a promising alternative to triple antibiotic paste for disinfecting non-vital young permanent teeth. How to cite this article Lillygrace E, Kethineni B, Puppala R, et al. Antimicrobial Efficacy of Triple Antibiotic Paste and Propolis as an Intracanal Medicament in Young Permanent Teeth: An In Vivo Study. Int J Clin Pediatr Dent 2021;14(2):243–248.

The Antibacterial Effect of Cysteamine and Its Combinations With Various Intracanal Medications Against Enterococcus Faecalis

“…Scrapping the dentin surface was done to have samples from deeper dentin levels. Paper points were used to absorb the transport fluid with dentin chips formed (15,29,30) .…”

Advances in the phytochemical screening and biological potential of propolis

“…Within the clinical applications of propolis, it is possible to mention the anticaries and microbial effect against oral pathogens [43]. Lillygrace et al [82] identified, from a randomized clinical trial, antimicrobial activity in dental treatment with intracanal application, with a reduction similar to the known conventional treatment.…”

A Potential Intracanal Medicament, 2-Hydroxyisocaproic Acid (HICA): Cytotoxicity, Genotoxicity, and Its Effect on SCAP Differentiation

“…Some studies showed significant increases in SCAP proliferation, while others showed no change in cytotoxicity depending on the concentration [8][9][10]. Propolis, a natural resin produced by honeybees, was shown to be significantly less cytotoxic than calcium hydroxide [14], while exhibiting antibacterial efficacy similar to that of TAP and calcium hydroxide [15][16][17], with additional antiviral, anti-inflammatory, and antioxidant properties [18]. Although propolis has good biocompatibility and antimicrobial effects, which makes it a good candidate for a natural intracanal medicament, it should be used with caution in vivo because of its allergenic potential [19].…”