Antimicrobial evaluation of the crude extract of symbiotic fungi from marine sponge Reniera japonica

Abstract

<p>Marine sponge-derived microbes are one of the rich sources of bioactive natural products with a broad spectrum of bioactivities. The present work focuses on the isolation and antimicrobial screening of the marine sponge-associated fungi from Reniera japonica MNP-2016. The results indicated that five fungi (L1-2, L2-1, L4, L8-1 and L14) were successfully isolated. Bioassay tests showed that only strain L14 had strong inhibitory effect on the pathogens, Staphyloccocus aureus, Escherichia coli and Candida albicans. 18S rDNA sequence analysis indicated that strain L14 was ascribed to Aspergillus genus. To the best of our knowledge, this work was the first report on the isolation and antimicrobial evaluation of fungi from R. japonica.</p><p><strong>Video Clip of Methodology</strong>:</p><p>1 min 13 sec: <a href="https://www.youtube.com/v/RSA2icw3emg">Full Screen</a> <a href="https://www.youtube.com/watch?v=RSA2icw3emg">Alternate</a></p>

EMNPD: a comprehensive endophytic microorganism natural products database for prompt the discovery of new bioactive substances

“…Endophytic microorganisms serve as a treasure trove of novel secondary metabolites, producing structurally diverse NPs with various potent biological activities. Particularly in the areas of antimicrobial and anticancer research [ 44 , 45 ], they offer new avenues for drug discovery. However, these valuable resources from endophytic microorganisms have yet to be fully utilized.…”

Natural Products from the Marine Sponge Subgenus Reniera

“…Therefore, great efforts should be made to carry out global resource surveys and collections of Reniera sponges and chemical studies using hyphenated technology, such as GC-MS and LC-MS-NMR. Furthermore, more attention should be paid on genome mining and the chemical study of symbiotic microorganisms of Reniera sponges as these microbes are potential producers of bioactive secondary metabolites originally derived from their hosts [ 56 , 57 , 58 ].…”

Genomic and AntiSMASH Analyses of Marine-Sponge-Derived Strain Aspergillus niger L14 Unveiling Its Vast Potential of Secondary Metabolites Biosynthesis

“…Strain L14 was isolated from one specimen (MNP-2016) of R. japonica , which was collected in September 2016 from the Xinghaiwan Coast of Dalian City, Liaoning Province, China [ 12 ]. One suspension of culture containing its mycelia in potato dextrose broth (PDB) supplemented with glycerol (20% v / v ) was stored at −80 °C.…”